One dry summer: A leaf proteome study on the response of oak to drought exposure

Sergeant, Kjell; Spieß, Nadine; Renaut, Jenny; Wilhelm, Eva; Hausman, J. F.

doi:10.1016/j.jprot.2011.03.011

Cited by 52 publications

(33 citation statements)

References 69 publications

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“…When high quality spectra were not matched to sequences, a sequence was determined manually and/or the spectra were used for searches allowing for semi-tryptic peptides and common post-translational modifications (PTMs). This resulted in the identification of several signal cleavage sites that were confirmed either by homology to known signal cleavage sites or by using predictive software [92]. …”

Section: Methodsmentioning

confidence: 99%

Differential Protein Expression in Response to Abiotic Stress in Two Potato Species: Solanum commersonii Dun and Solanum tuberosum L.

Folgado

Panis

Sergeant³

et al. 2013

IJMS

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Better knowledge on responses to dehydration stress could help to improve the existing cryopreservation protocols for potato, since plant tissues processed for cryopreservation are often submitted to similar in vitro stress conditions. Cryopreservation (the best method of conservation for vegetatively propagated plants) of potato still needs to be standardized to make it available and to conserve the wide diversity of this crop. In the present work, the response to osmotic stress and chilling temperature was investigated in two potato species, Solanum tuberosum and its relative, frost-tolerant S. commersonii. After 14 days of exposure, different growth parameters, such as shoot length and number of leaves, were measured. Furthermore, differentially abundant proteins were identified after performing 2-fluorescence difference gel electrophoresis (2-DIGE) experiments, and soluble carbohydrates were analyzed by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD). The results show different responses in both species depending on the stress treatment. Focusing on the differences in growth parameters during the treatments, Solanum commersonii seems to be more affected than S. tuberosum cv. Désirée. At the molecular level, there are some differences and similarities between the two potato species studied that are dependent on the type of stressor.

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Section: Methodsmentioning

confidence: 99%

Differential Protein Expression in Response to Abiotic Stress in Two Potato Species: Solanum commersonii Dun and Solanum tuberosum L.

Folgado

Panis

Sergeant³

et al. 2013

IJMS

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“…The increase of CRT abundance enhanced the survival of T. aestivum plants under drought condition [77], and the TaCRT -overexpressing tobacco ( Nicotiana benthamiana ) plants exhibited enhanced drought resistance [78]. Proteomic studies revealed a drought-induced CRT1 in Glycine max [17], but a drought-reduced CRT1 in Quercus robur under prolonged (65 days) drought stress [61]. These results indicate that CRT functions in drought tolerance in a stress intensity-dependent manner.…”

Section: Drought Sensing and Signalingmentioning

confidence: 99%

Drought-Responsive Mechanisms in Plant Leaves Revealed by Proteomics

Wang

Cai

et al. 2016

IJMS

213

143

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Plant drought tolerance is a complex trait that requires a global view to understand its underlying mechanism. The proteomic aspects of plant drought response have been extensively investigated in model plants, crops and wood plants. In this review, we summarize recent proteomic studies on drought response in leaves to reveal the common and specialized drought-responsive mechanisms in different plants. Although drought-responsive proteins exhibit various patterns depending on plant species, genotypes and stress intensity, proteomic analyses show that dominant changes occurred in sensing and signal transduction, reactive oxygen species scavenging, osmotic regulation, gene expression, protein synthesis/turnover, cell structure modulation, as well as carbohydrate and energy metabolism. In combination with physiological and molecular results, proteomic studies in leaves have helped to discover some potential proteins and/or metabolic pathways for drought tolerance. These findings provide new clues for understanding the molecular basis of plant drought tolerance.

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“…Samples (0.7 μL) were then spotted on MALDI-TOF target plates (Applied Biosystems), before the deposit of 0.7 μL CHCA (7 mg mL -1 in ACN 50%, TFA 0.1%). Peptide mass determinations were carried out using the Applied Biosystems 5800 Proteomics Analyzer tryptophan (single oxidation, double oxidation and kynurenin) were set as variable modifications, as previously described [30,31]. Homology identification was retained with probability set at 95 %.…”

Section: Protein Identificationmentioning

confidence: 99%

Investigating Aspergillus nidulans secretome during colonisation of cork cell walls

Martins

Garcia

Varela

et al. 2014

Journal of Proteomics

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Cork, the outer bark of Quercus suber, shows a unique compositional structure, a set of remarkable properties, including high recalcitrance. Cork colonisation by Ascomycota remains largely overlooked. Herein, Aspergillus nidulans secretome on cork was analysed (2DE).Proteomic data were further complemented by microscopic (SEM) and spectroscopic (ATR-FTIR) evaluation of the colonised substrate and by targeted analysis of lignin degradation compounds (UPLC-HRMS). Data showed that the fungus formed an intricate network of hyphae around the cork cell walls, which enabled polysaccharides and lignin superficial degradation, but probably not of suberin. The degradation of polysaccharides was suggested by the identification of few polysaccharide degrading enzymes (β-glucosidases and endo-1,5--L-arabinosidase).Lignin degradation, which likely evolved throughout a Fenton-like mechanism relying on the activity of alcohol oxidases, was supported by the identification of small aromatic compounds (e.g. cinnamic acid and veratrylaldehyde) and of several putative high molecular weight lignin degradation products. In addition, cork recalcitrance was corroborated by the identification of several protein species which are associated with autolysis. Finally, stringent comparative proteomics revealed that A. nidulans colonisation of cork and wood share a common set of enzymatic mechanisms. However the higher polysaccharide accessibility in cork might explain the increase of β-glucosidase in cork secretome.

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One dry summer: A leaf proteome study on the response of oak to drought exposure

Cited by 52 publications

References 69 publications

Differential Protein Expression in Response to Abiotic Stress in Two Potato Species: Solanum commersonii Dun and Solanum tuberosum L.

Differential Protein Expression in Response to Abiotic Stress in Two Potato Species: Solanum commersonii Dun and Solanum tuberosum L.

Drought-Responsive Mechanisms in Plant Leaves Revealed by Proteomics

Investigating Aspergillus nidulans secretome during colonisation of cork cell walls

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