One‐minute exposure of 4‐cell mouse embryos to glucose overcomes morula block in CZB medium

Chatot, Clare L.; Lewis-Williams, J.; Torres, Ioannis; Ziomek, Carol A.

doi:10.1002/mrd.1080370407

Cited by 40 publications

(51 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This hypothesis was confirmed by recent finding showing the influence of oil on embryo development during in vitro-culture [12]. In addition, a Chatot, Ziomek and Bavister (CZB) medium [1][2][3][4] was employed as a basic medium of our culture system, but this does not contain several substrates having embryotrophic action reported in previous studies [8][9][10][11].…”

supporting

confidence: 81%

“…Culture medium: The basic medium used for the culture of embryos was CZB medium, consisting of NaCl (81.6 mM), KCl (4.8 mM), KH 2 PO 4 (1.2 mM), MgSO 4 •7H 2 O (1.2 mM), CaCl 2 •2H 2 O (1.7 mM), NaHCO 3 (25.1 mM), sodium lactate (31.3 mM), sodium pyruvate (0.3 mM), glutamine (1 mM), EDTA (0.1 mM), and BSA (5 mg/ml). According to the experimental design, glucose (5.6 mM) and/or Hb (0.001 mg/ml, methemoglobin type) were added to CZB medium.…”

Section: Collection Of Embryosmentioning

confidence: 99%

See 1 more Smart Citation

Renovation of a Drop Embryo Cultures System by Using Refined Mineral oil and the Effect of Glucose and/or Hemoglobin Added to a Serum-free Medium

et al. 2004

View full text Add to dashboard Cite

ABSTRACT. This study was conducted to evaluate whether refining mineral oil and the addition of hemoglobin and/or glucose to a serumfree medium could improve in vitro-development of embryos cultured in a chemicallysemi-defined microdroplet culture system. Block strain, outbred (ICR) mouse 1-or 2-cell embryos were cultured in 5 µl droplets of Chatot, Ziomek and Bavister medium overlaid with mineral oil of different types, and preimplantation development to the blastocyst stage was subsequently monitored. In the experiment 1, either Sigma (M-8410) or BDH (GPR TM ) mineral oil with or without washing was used for embryo culture and, distilled water (DW) or culture medium was used as a washing agent. As results, better (P<0.0001) development of 1-cell embryos was found in the Sigma than in the BDH; more blastocysts developed in Sigma oil washed with culture medium than in the others (37% vs. 0%). Subsequent ly, 1-(experiment 2) or 2-cell (experiment 3) embryos were cultured in the droplets overlaid with medium-washed Sigma oil, to which 0.001 mg/ml hemoglobin and/or 5.6 mM glucose were supplemented at the 1-cell and the 4-cell stages, respectively. Regardless of embryo stages, blastocyst formation was significantly improved by the addition of hemoglobin (54 to 48% vs. 42 to 31% in 1-cell and 83 to 78% vs. 65 to 68% in 2-cell embryos) and this effect was independent of glucose addition. In conclusion, the selection and washing of mineral oil, and the addition of hemoglobin is beneficial for improving the efficacy of a drop embryo culture system using a serum-free medium. KEY WORDS: glucose, hemoglobin, mineral oil, mouse embryo, preimplantation development.J. Vet. Med. Sci. 66(1): 63-66, 2004 Technical advance in gamete manipulation is fundamental for developing novel medical biotechnologies. Lots of information has been obtained from various model studies and we have developed the mouse model system of embryo culture employing serum-free media and outbred strain (Institute of Cancer Research; ICR) [10,11]. Subsequently, a culture technique using small microdroplet of a serumfree medium has been developed. In this system, commercially-purchased mineral oil is used for preparing microdroplets, so culture medium was continuously exposes to overlaid oil throughout the culture. It is possible that the artifacts included in overaid oil directly affect on the development of embryos cultured in microdroplet by continuous exposure during in vitro-culture. This hypothesis was confirmed by recent finding showing the influence of oil on embryo development during in vitro-culture [12]. In addition, a Chatot, Ziomek and Bavister (CZB) medium [1-4] was employed as a basic medium of our culture system, but this does not contain several substrates having embryotrophic action reported in previous studies [8][9][10][11].Consequently, a randomized, prospective study was designed to further improve our embryo culture system using the microdroplet of a serum-free medium. This study was conducted to examine 1) the effects of two types ...

show abstract

supporting

confidence: 81%

Section: Collection Of Embryosmentioning

confidence: 99%

Renovation of a Drop Embryo Cultures System by Using Refined Mineral oil and the Effect of Glucose and/or Hemoglobin Added to a Serum-free Medium

et al. 2004

View full text Add to dashboard Cite

show abstract

“…It has been known for over a decade that, although murine precompaction stage embryos are unable to metabolize glucose, they require a brief exposure to glucose prior to the morul stage (24). Without this glucose exposure, the embryos undergo increased apoptosis, decreased proliferation, and reduced glucose transport, and their progression to a blastocyst stage is impaired.…”

Section: Glut3 In the Embryomentioning

confidence: 99%

The facilitative glucose transporter GLUT3: 20 years of distinction

Simpson

Dwyer

Malide

et al. 2008

American Journal of Physiology-Endocrinology and Metabolism

404

322

View full text Add to dashboard Cite

Glucose metabolism is vital to most mammalian cells, and the passage of glucose across cell membranes is facilitated by a family of integral membrane transporter proteins, the GLUTs. There are currently 14 members of the SLC2 family of GLUTs, several of which have been the focus of this series of reviews. The subject of the present review is GLUT3, which, as implied by its name, was the third glucose transporter to be cloned (Kayano T, Fukumoto H, Eddy RL, Fan YS, Byers MG, Shows TB, Bell GI. J Biol Chem 263: [15245][15246][15247][15248] 1988) and was originally designated as the neuronal GLUT. The overriding question that drove the early work on GLUT3 was why would neurons need a separate glucose transporter isoform? What is it about GLUT3 that specifically suits the needs of the highly metabolic and oxidative neuron with its high glucose demand? More recently, GLUT3 has been studied in other cell types with quite specific requirements for glucose, including sperm, preimplantation embryos, circulating white blood cells, and an array of carcinoma cell lines. The last are sufficiently varied and numerous to warrant a review of their own and will not be discussed here. However, for each of these cases, the same questions apply. Thus, the objective of this review is to discuss the properties and tissue and cellular localization of GLUT3 as well as the features of expression, function, and regulation that distinguish it from the rest of its family and make it uniquely suited as the mediator of glucose delivery to these specific cells.neurons; sperm; preimplantation embryo; white blood cells GLUCOSE METABOLISM IS VITAL to most mammalian cells, and the passage of glucose across cell membranes is facilitated by a family of integral membrane transporter proteins, the GLUTs. There are currently 14 members of the SLC2 family of GLUTs, several of which have been the focus of this series of reviews. The subject of the present review is GLUT3 which, as implied by its name, was the third glucose transporter to be cloned (62) and was originally designated as the neuronal glucose transporter. Together with GLUT1, -2, and -4, it comprises the Class 1 group of transporters (For review see Refs. 15,81,121). With the cloning of GLUT3, it became apparent that the brain did not rely exclusively on GLUT1 and that GLUT3 was highly and specifically expressed by neurons. Thus, GLUT3 became the third facilitative glucose transporter isoform with unique characteristics suited for cell-specific expression and function. GLUT2 is ideally suited for expression in liver and pancreas due to its high K m for glucose; GLUT4 and its translocation from intracellular vesicles to the cell surface facilitates insulin-stimulated glucose uptake in insulin-sensitive cells: muscle and fat. The overriding question that drove the early work in GLUT3 in the brain was: why would neurons need a separate glucose transporter isoform; what is it about GLUT3 that specifically suits the needs of the highly metabolic and oxidative neuron with its high glucose demand?...

show abstract

“…Brief exposure of embryos to H 2 O 2 inhibits cell proliferation and causes arrest before blastulation (Cebral et al 2007). Similar consequences follow from early periods of glucose deprivation in vitro (Sakkas et al 1989, Brown & Whittingham 1991, Chatot et al 1994, Martin & Leese 1995. At the heart of peroxisomal proliferation is the activity of the peroxisome proliferator-activated receptors (PPAR).…”

Section: Introductionmentioning

confidence: 97%

Glucose deprivation, oxidative stress and peroxisome proliferator-activated receptor-α (PPARA) cause peroxisome proliferation in preimplantation mouse embryos

Jansen

Cashman²,

Thompson³

et al. 2009

Reproduction

View full text Add to dashboard Cite

Ex vivo two-cell mouse embryos deprived of glucose in vitro can develop to blastocysts by increasing their pyruvate consumption; however, zygotes when glucose-deprived cannot adapt this metabolic profile and degenerate as morulae. Prior to their death, these glucose-deprived morulae exhibit upregulation of the H C -monocarboxylate co-transporter SLC16A7 and catalase, which partly co-localize in peroxisomes. SLC16A7 has been linked to redox shuttling for peroxisomal b-oxidation. Peroxisomal function is unclear during preimplantation development, but as a peroxisomal transporter in embryos, SLC16A7 may be involved and influenced by peroxisome proliferators such as peroxisome proliferator-activated receptor-a (PPARA). PCR confirmed Ppara mRNA expression in mouse embryos. Zygotes were cultured with or without glucose and with the PPARA-selective agonist WY14643 and the developing embryos assessed for expression of PPARA and phospho-PPARA in relation to the upregulation of SLC16A7 and catalase driven by glucose deprivation, indicative of peroxisomal proliferation. Reactive oxygen species (ROS) production and relationship to PPARA expression were also analysed. In glucose-deprived zygotes, ROS was elevated within 2 h, as were PPARA expression within 8 h and catalase and SLC16A7 after 12-24 h compared with glucose-supplied embryos. Inhibition of ROS production prevented this induction of PPARA and SLC16A7. Selective PPARA agonism with WY14643 also induced SLC16A7 and catalase expression in the presence of glucose. These data suggest that glucose-deprived cleavage stage embryos, although supplied with sufficient monocarboxylate-derived energy, undergo oxidative stress and exhibit elevated ROS, which in turn upregulates PPARA, catalase and SLC16A7 in a classical peroxisomal proliferation response.

show abstract

One‐minute exposure of 4‐cell mouse embryos to glucose overcomes morula block in CZB medium

Cited by 40 publications

References 27 publications

Renovation of a Drop Embryo Cultures System by Using Refined Mineral oil and the Effect of Glucose and/or Hemoglobin Added to a Serum-free Medium

Renovation of a Drop Embryo Cultures System by Using Refined Mineral oil and the Effect of Glucose and/or Hemoglobin Added to a Serum-free Medium

The facilitative glucose transporter GLUT3: 20 years of distinction

Glucose deprivation, oxidative stress and peroxisome proliferator-activated receptor-α (PPARA) cause peroxisome proliferation in preimplantation mouse embryos

Contact Info

Product

Resources

About