One-pot gram-scale synthesis of virucidal heparin-mimicking polymers as HSV-1 inhibitors

Ahmadi, Vahid; Nie, Chuanxiong; Mohammadifar, Ehsan; Achazi, Katharina; Wedepohl, Stefanie; Kerkhoff, Yannic; Block, Stephan; Osterrieder, Nikolaus; Haag, Rainer

doi:10.1039/d1cc04703e

Cited by 16 publications

(17 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the antiviral activity of this carrageenan observed during the pre-treatment of the virus with SPs was higher. This activity is related to the inhibition of the virus/cell interactions and could be similar to other sulfated polysaccharides, such as heparin, an SPs with an extracellular mechanism that shows an inhibitory effect for a variety of viruses by interacting with their surface, and the broad antiviral activity of heparin that has allowed the development of numerous heparin-mimetic compounds [56]. Carrageenans from G. atropurpurea [30] and G. skottsbergii [34] showed higher antiviral activity than heparin.…”

Section: Antiviral Activity Of Sulfated Polysaccharidesmentioning

confidence: 81%

Sulfated Polysaccharides from Seaweed Strandings as Renewable Source for Potential Antivirals against Herpes simplex Virus 1

et al. 2022

View full text Add to dashboard Cite

Herpes simplex virus 1 (HSV-1) remains a prominent health concern widespread all over the world. The increasing genital infections by HSV-1 that might facilitate acquisition and transmission of HIV-1, the cumulative evidence that HSV-1 promotes neurodegenerative disorders, and the emergence of drug resistance signify the need for new antiviral agents. In this study, the in vitro anti-herpetic activity of sulfated polysaccharides (SPs) extracted by enzyme or hot water from seaweeds collected in France and Mexico from stranding events, were evaluated. The anti-herpetic activity evaluation of the semi-refined-polysaccharides (sr-SPs) and different ion exchange purified fractions showed a wide range of antiviral activity. Among them, the sr-SPs from the Rhodophyta Halymenia floresii showed stronger activity EC50 0.68 μg/mL with SI 1470, without cytotoxicity. Further, the antiviral activity of the sr-SPs evaluated at different treatment schemes showed a high EC50 of 0.38 μg/mL during the viral adsorption assays when the polysaccharide and the virus were added simultaneously, whilst the protection on Vero cell during the post-infection assay was effective up to 1 h. The chemical composition, FTIR and 1H NMR spectroscopic, and molecular weights of the sr-SPs from H. floresii were determined and discussed based on the anti-herpetic activity. The potential utilization of seaweed stranding as a source of antiviral compounds is addressed.

show abstract

Section: Antiviral Activity Of Sulfated Polysaccharidesmentioning

confidence: 81%

Sulfated Polysaccharides from Seaweed Strandings as Renewable Source for Potential Antivirals against Herpes simplex Virus 1

et al. 2022

View full text Add to dashboard Cite

show abstract

“…In addition to mere quantification of viral infection, our analysis macro can also be applied to quantify the efficiency of virus binding inhibitors [17] . This is demonstrated in Fig.…”

Section: Methods Detailsmentioning

confidence: 99%

A fast open-source Fiji-macro to quantify virus infection and transfection on single-cell level by fluorescence microscopy

et al. 2022

Self Cite

View full text Add to dashboard Cite

“… 153 Recently, synthetic modifications of DPGS and HPGS accomplished by introducing hydrophobic alkyl motifs and sulfation have provided for a new class of antivirals with both inhibitory and virucidal properties. 215 − 217 …”

Section: Hs Mimetics As Viral Inhibitorsmentioning

confidence: 99%

“…214 In the past years, Haag and co-workers developed a variety of polysulfated materials and polymers as HP mimetics with great antiviral potential. [153][154][155]215,216 Polyglycerol sulfate (PGS), originally developed as an HP analogue with anticoagulant and anticomplement activity, was rapidly identified as a potential antiviral agent (see Figure 3). Polyglycerol-based systems as antivirals are especially interesting due to their inherent biocompatibility and lower anticoagulant activity compared to HP.…”

Section: Role Of Hp and Hs In Viralmentioning

confidence: 99%

Polymers Inspired by Heparin and Heparan Sulfate for Viral Targeting

2022

View full text Add to dashboard Cite

Heparin (HP) and heparan sulfate (HS) are linear, anionically charged polysaccharides well-known for their diverse biological activities. While HP is generally localized in mast cells and in connective tissues, HS is part of the glycocalyx and involved in the attachment of viruses to host cells, constituting the first step of an infection. HP and HS also exhibit antiviral activity by blocking viral receptors, thereby inhibiting viruses from engaging with host cells. Inspired by their structural features, such as their high molecular weight and polyanionic character, various synthetic polymers mimicking HP/HS have been developed and used as model systems to study bioactivity, as well as for therapeutic applications. This Perspective provides an overview of the roles of HP/HS in viral engagement, and examines historical and recent approaches toward oligo-/polysaccharide, glycopolymer, and anionic polymer HP/HS mimetics. An overview of current applications and future prospects of these molecules is provided, demonstrating their potential in addressing current and future epidemics and pandemics.

show abstract

One-pot gram-scale synthesis of virucidal heparin-mimicking polymers as HSV-1 inhibitors

Abstract: A straightforward and gram-scale synthesis method was developed to engineer virucidal heparin-mimicking polymers as HSV-1 inhibitors.

Cited by 16 publications

References 25 publications

Sulfated Polysaccharides from Seaweed Strandings as Renewable Source for Potential Antivirals against Herpes simplex Virus 1

Sulfated Polysaccharides from Seaweed Strandings as Renewable Source for Potential Antivirals against Herpes simplex Virus 1

A fast open-source Fiji-macro to quantify virus infection and transfection on single-cell level by fluorescence microscopy

Polymers Inspired by Heparin and Heparan Sulfate for Viral Targeting

Contact Info

Product

Resources

About