One-pot synthesis of dextran-coated iron oxide nanoclusters for real-time regional lymph node mapping

Fu, Chaoping; Zhou, Huixia; Wang, Yanan; Liu, Dong; Li, Junmeng; Deng, Haijun; Qi, Xiaolong; Chen, Tao; Zhang, Liming; Li, Guoxin

doi:10.2147/ijn.s130322

Cited by 20 publications

(10 citation statements)

References 19 publications

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“…In addition, Hauser et al found that synthetic methods, the amount of dextran can greatly affect the properties of the IONPs such as size, stability, crystallinity and magnetism [ 172 ]. Owing to its biosafety, bioactivity, biocompatibility, low cytotoxicity, dextran-coated IONPs are considered promising candidates for biomedical applications [ 173 ]. Unterweger et al developed a novel drug delivery system by coating IONPs with dextran and cisplatin hyaluronic acid.…”

Section: Surface Modification Of Magnetic Iron Oxide Nanoparticlesmentioning

confidence: 99%

Surface Modification of Magnetic Iron Oxide Nanoparticles

et al. 2018

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Functionalized iron oxide nanoparticles (IONPs) are of great interest due to wide range applications, especially in nanomedicine. However, they face challenges preventing their further applications such as rapid agglomeration, oxidation, etc. Appropriate surface modification of IONPs can conquer these barriers with improved physicochemical properties. This review summarizes recent advances in the surface modification of IONPs with small organic molecules, polymers and inorganic materials. The preparation methods, mechanisms and applications of surface-modified IONPs with different materials are discussed. Finally, the technical barriers of IONPs and their limitations in practical applications are pointed out, and the development trends and prospects are discussed.

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Section: Surface Modification Of Magnetic Iron Oxide Nanoparticlesmentioning

confidence: 99%

Surface Modification of Magnetic Iron Oxide Nanoparticles

et al. 2018

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“…Although WBC and PLT fluctuated among the three tests, no symptom of infection or anemia was found. Liver and kidney function and blood lipids were normal [ 34 ], as were GLU levels. Levels of immune-related factors and immune cells in the blood and spleen were also within the normal range.…”

Section: Discussionmentioning

confidence: 99%

Study on the Safety of Human Oligodendrocyte Precursor Cell Transplantation in Young Animals and Its Efficacy on Myelination

Zhou

et al. 2021

Stem Cells and Development

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Oligodendrocyte precursor cells (OPCs), which can differentiate into myelinating oligodendrocytes during embryonic development, are an important potential source for myelin repair or regeneration. To date, OPCs from human sources (hOPCs) remain limited. In this study, we aimed to evaluate the safety and remyelination capacity of hOPCs developed in our laboratory by transplanting them into the lateral ventricles of Sprague-Dawley rats of different ages. The toxicity, biodistribution, and tumor formation abilities of the injected hOPCs were examined by evaluating rats' vital signs, developmental indicators, neural re exes, along with hematological, immunological, and pathological assessments. In addition, the hOPCs were transplanted into the corpus callosum of shiverer mice to verify cell myelination e cacy.Overall, our results showed that transplanted hOPCs into young mice showed no toxicity against their organ function or immune system, engrafted only in the brain, and caused no tissue proliferation or tumor formation. In terms of e cacy, the transplanted hOPCs formed myelin in the corpus callosum, alleviated the trembling phenotype of shiverer mice, and promoted normal development. The transplantation of hOPCs is safe and can effectively form myelin in the brain, thereby providing a theoretical basis for the future clinical transplantation of hOPCs.

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“…At least three experiments were performed, and each was tested in triplicate. The experiment was repeated three times independently in the laboratory [ 25 ].…”

Section: Methodsmentioning

confidence: 99%

Identifying the functions of two biomarkers in human oligodendrocyte progenitor cell development

Zhou

Wang

et al. 2021

J Transl Med

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Background Human oligodendrocyte precursor cells (hOPCs) are an important source of myelinating cells for cell transplantation to treat demyelinating diseases. Myelin oligodendrocytes develop from migratory and proliferative hOPCs. It is well known that NG2 and A2B5 are important biological markers of hOPCs. However, the functional differences between the cell populations represented by these two biomarkers have not been well studied in depth. Objective To study the difference between NG2 and A2B5 cells in the development of human oligodendrocyte progenitor cells. Methods Using cell sorting technology, we obtained NG2+/−, A2B5+/− cells. Further research was then conducted via in vitro cell proliferation and migration assays, single-cell sequencing, mRNA sequencing, and cell transplantation into shiverer mice. Results The proportion of PDGFR-α + cells in the negative cell population was higher than that in the positive cell population. The migration ability of the NG2+/−, A2B5+/− cells was inversely proportional to their myelination ability. The migration, proliferation, and myelination capacities of the negative cell population were stronger than those of the positive cell population. The ability of cell migration and proliferation of the four groups of cells from high to low was: A2B5− > NG2− > NG2+ > A2B5+. The content of PDGFR-α+ cells and the ability of cell differentiation from high to low was: NG2− > A2B5− > A2B5+ > NG2+. Conclusion In summary, NG2+ and A2B5+ cells have poor myelination ability due to low levels of PDGFR-α+ cells. Therefore, hOPCs with a higher content of PDGFR-α+ cells may have a better effect in the cell transplantation treatment of demyelinating diseases.

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One-pot synthesis of dextran-coated iron oxide nanoclusters for real-time regional lymph node mapping

Cited by 20 publications

References 19 publications

Surface Modification of Magnetic Iron Oxide Nanoparticles

Surface Modification of Magnetic Iron Oxide Nanoparticles

Study on the Safety of Human Oligodendrocyte Precursor Cell Transplantation in Young Animals and Its Efficacy on Myelination

Identifying the functions of two biomarkers in human oligodendrocyte progenitor cell development

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