One-Step Electrosynthesis of Graphene Oxide-Doped Polypyrrole Nanocomposite as a Nanointerface for Electrochemical Impedance Detection of Cell Adhesion and Proliferation Using Two Approaches

Abstract

A novel nanointerface of graphene oxide-doped polypyrrole (GO/PPy) is prepared on the surface of an indium tin oxide (ITO) electrode for electrochemical impedance detection of cell adhesion and proliferation through a facile one-step electropolymerization. The prepared GO/PPy nanocomposite had a robust surface and provided a biocompatible substrate for A549 cells adhesion and proliferation. The adhesion and proliferation of A549 cells on the surface of the GO/PPy modified ITO electrode directly increased the electron transfer resistance of [Fe(CN)6]3−/4−redox probe and influenced the impedance properties of the GO/PPy modified ITO electrode system. Based on these results, the adhesion and proliferation of A549 cells could be detected by electrochemical impedance technology using two approaches. Therefore, the present paper confirms that the GO/PPy nanocomposite film provides an excellent biological-electrical interface for cell immobilization and offers advantages of simple, low-cost fabrication and multiparameter detection and possesses potential application in cytological studies.

Noninvasive Detection of Ammonia in the Breath of Hemodialysis Patients Using a Highly Sensitive Ammonia Sensor Based on a Polypyrrole/Sulfonated Graphene Nanocomposite

“…Structural configuration analysis and the characteristic vibrational bands of the components (GO, RGO, SRGO, and PPy) and their composites (PPy/GO, PPy/RGO, PPy/SRGO) were characterized using FT-IR spectroscopy in the range of 4000–400 cm –1 (Figure ). The peaks at 1542 and 1457 cm –1 for PPy are assigned to the C–N and C–C stretching vibrations of the pyrrole ring, respectively. , The broad band at 3417 cm –1 is attributed to the N–H stretching vibration . The FT-IR spectrum of GO shows a peak at 1727 cm –1 , which is attributed to the CO stretching of the carboxylic groups present at the edges of the GO sheets .…”

“…The peaks at 1542 and 1457 cm −1 for PPy are assigned to the C−N and C−C stretching vibrations of the pyrrole ring, respectively. 35,36 The broad band at 3417 cm −1 is attributed to the N−H stretching vibration. 37 The FT-IR spectrum of GO shows a peak at 1727 cm −1 , which is attributed to the CO stretching of the carboxylic groups present at the edges of the GO sheets.…”

Preparation of Electrochemical Supercapacitor Based on Polypyrrole/Gum Arabic Composites

“…The signal at 1703 cm −1 is due to the out-of-plane wagging of the carbonyl group. At 1035 cm −1 , the stretching vibration of C-H of the Py ring can be noticed [30,31]. The peak at 1291 cm −1 is connected to C-N in a plane.…”

Preparation of Graphene Oxide-Doped Polypyrrole Composite Films with Stable Conductivity and Their Effect on the Elongation and Alignment of Neurite

“…Sodium dodecyl benzenesulfonate (DBS) was usually doped into PPy for many tissue engineering fields, including nerve regeneration, because of the biocompatibility, doping stability, and higher conductivity in PPy. − To further improve the conductivity of nerve repair materials, researchers have also applied graphene and its derivatives to biomaterials in recent years, including graphene oxide (GO) and carboxyl functionalized graphene oxide (CFGO) because of their good conductivity and modulation of cellular behavior, − leading to the preparation of the conductive PPy materials doped with graphene or its derivatives. , Yan et al reported that polypyrrole/graphene electrodes via electrical stimulation could improve neurite outgrowth and antiaging ability of retinal ganglion cells . And Feng et al.…”

“…22−24 To further improve the conductivity of nerve repair materials, researchers have also applied graphene and its derivatives to biomaterials in recent years, including graphene oxide (GO) and carboxyl functionalized graphene oxide (CFGO) because of their good conductivity and modulation of cellular behavior, 25−28 leading to the preparation of the conductive PPy materials doped with graphene or its derivatives. 29,30 Yan et al reported that polypyrrole/graphene electrodes via electrical stimulation could improve neurite outgrowth and antiaging ability of retinal ganglion cells. 29 And Feng et al found that the graphene nanofibers could accelerate the nerve growth via electrical stimulation.…”