One-step nucleic acid amplification assay is an accurate technique for sentinel lymph node biopsy of breast cancer patients: a meta-analysis

Shi, Fang; Zhang, Qian; Liang, Zhenzhen; Zhang, Mengmeng; Liu, Xin

doi:10.1038/bjc.2017.262

Cited by 26 publications

(13 citation statements)

References 27 publications

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“…It can significantly minimize the sampling limitation compared to histological evaluations. Based on the patients and nodes, the good performance of the assay in this study is similar to other published researches and meta-analysis 20–23. Thirdly, the construction of existing models was based on postoperative histological information, including tumor grade, multi-focus and vascular invasion, etc., which means that it is impossible to adopt the existing model in the operation to guide the subsequent axillary operation.…”

Section: Discussionsupporting

confidence: 76%

<p>Intraoperative Prediction Of Non-Sentinel Lymph Node Metastasis Based On The Molecular Assay In Breast Cancer Patients</p>

Sun

Zhang

et al. 2019

CMAR

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PurposeThe aim of the study is to construct an intraoperative nomogram for the prediction of non-sentinel lymph node (NSLN) metastasis based on the one-step nucleic acid amplification assay in breast cancer patients.MethodsA total of 552 patients were enrolled in the training study and 1090 patients were enrolled in the validation study. The nomogram was constructed based on the molecular assay with logistic multivariate regression analysis in the training study and was validated in the validation study.ResultsA novel nomogram model was constructed with the total tumor load, the clinical primary tumor size, the number of positive and negative sentinel lymph nodes. The area under the receiver operating characteristic curve (AUC) of the model was 0.842. The AUC of the model which was sensitive to discern the patients with the stage of pN1 and ≥pN2 was 0.861.ConclusionThe nomogram model will help to guide the axillary management intraoperatively and precisely confirm the target region of radiotherapy postoperatively.

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Section: Discussionsupporting

confidence: 76%

<p>Intraoperative Prediction Of Non-Sentinel Lymph Node Metastasis Based On The Molecular Assay In Breast Cancer Patients</p>

Sun

Zhang

et al. 2019

CMAR

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“…It's interesting to note that, according to our study, also in the other studies positive predictive value is constantly lower than negative predictive value. This finding had already been extensively reported and discussed from Shi et colleagues in a metanalysis on OSNA in breast cancer on 2833 patients [30]. As already discussed, this fact is likely to be related to the lower sensitivity of classic histopathology examination than OSNA.…”

Section: Discussionsupporting

confidence: 56%

“…This technique allows to quickly isolate, amplify and quantify mRNA encoding for CK-19; a single examination takes 30–40 min, is highly automated, and does not require sterile working conditions: that makes OSNA suitable for intraoperative examination. The excellent accuracy of OSNA in detecting node metastases in breast cancer has been shown in several clinical studies [[26], [27], [28], [29], [30]]. Later, OSNA has been shown effective in detecting lymph node metastases from other types of tumors, such as colorectal carcinoma, endometrial carcinoma, gastric carcinoma, head and neck squamous cell carcinoma and lung carcinoma [[31], [32], [33], [34]].…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of accuracy of one-step nucleic acid amplification (OSNA) in diagnosis of lymph node metastases of papillary thyroid carcinoma. Diagnostic study

Medas

Coni

Podda

et al. 2019

Annals of Medicine and Surgery

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Background The incidence of node metastases in papillary thyroid carcinoma (PTC) is high, ranging from 20% to 90%. Prophylactic central lymph node compartment dissection (CLND), suggested from the latest guidelines for high-risk tumors, meets resistance due to the high incidence of postoperative complications. Recently, new molecular biologic techniques, such as One Step Nucleic Acid Amplification (OSNA), have spread widely, allowing to quickly isolate, amplify and quantify mRNA encoding for proteins selectively present in neoplastic cells, as Cytokeratine-19. The aim of this study is to evaluate the application of OSNA to intraoperative diagnosis of node metastases of PTC. Methods We included in the study patients with preoperative diagnosis of PTC; from each patient one or more lymph nodes were collected. To assess OSNA accuracy, each lymph node was divided into two halves: the first one was analysed with histopathological and immunohistochemical examination, whereas the second was studied with OSNA. Results Twenty-six lymph nodes from 13 patients were included in the study. Overall, OSNA sensitivity was 87.5%, specificity 94.4%, positive predictive value 87.5%, negative predictive value 94.4% and accuracy 92.8%. Discussion and conclusion OSNA is effective in detecting lymph node metastases of PTC. Considering the high risk of complications in CLND, and the uncertain prognostic value of lymph node metastases of PTC, OSNA seems to be a promising tool to identify intraoperatively patients who may benefit from CLND.

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“…Поэтому обнаружение цитокератина 19 в ЛУ является признаком присутствия в нем опухолевых клеток эпителиального происхождения. Метод был разработан для детекции метастазов рака молочной железы и с тех пор успешно применен для детекции метастазов других опухолей эпителиального происхождения, в частности колоректального рака, немелкоклеточного рака легких, рака матки и щитовидной железы [21][22][23][24].…”

Section: визуализация продуктов реакцииunclassified

Loop-mediated isothermal amplification: an effective method for express-diagnostics of cancer

Макарова¹,

Зотиков²,

Белякова³

et al. 2018

Cancer Urology

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The review is devoted to loop-mediated isothermal amplification (LAMP) – a novel molecular diagnostic method that has recently become increasingly popular. Unlike polymerase chain reaction, LAMP does not require thermal cycling; DNA or RNA amplification occurs at a constant temperature (about 65 °C) with 4 or 6 primers. This is a fast, highly-sensitive, and highly specific method, which does not require expensive equipment, where visual detection of the reaction products is performed by the unaided eye. LAMP is successfully used for the diagnosis of multiple viruses, bacteria, and other pathogens (including those in food). Moreover, it can be applied for the detection of singlenucleotide polymorphisms. Recently, a modified LAMP assay – one-step nucleic acid amplification (OSNA) – was validated for metastasis detection. OSNA was demonstrated to have almost the same sensitivity and specificity as standard diagnostic methods (sometimes even higher). Particular attention is paid to the mechanism of LAMP, primer design, and diagnostics of cancer using OSNA.

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One-step nucleic acid amplification assay is an accurate technique for sentinel lymph node biopsy of breast cancer patients: a meta-analysis

Cited by 26 publications

References 27 publications

<p>Intraoperative Prediction Of Non-Sentinel Lymph Node Metastasis Based On The Molecular Assay In Breast Cancer Patients</p>

<p>Intraoperative Prediction Of Non-Sentinel Lymph Node Metastasis Based On The Molecular Assay In Breast Cancer Patients</p>

Evaluation of accuracy of one-step nucleic acid amplification (OSNA) in diagnosis of lymph node metastases of papillary thyroid carcinoma. Diagnostic study

Loop-mediated isothermal amplification: an effective method for express-diagnostics of cancer

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