One-Step Purification of Microbially Produced Hydrophobic Terpenes via Process Chromatography

Grozdev, L; Kaiser, Johann; Berensmeier, Sonja

doi:10.3389/fbioe.2019.00185

Cited by 7 publications

(6 citation statements)

References 64 publications

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“…In the case of SLPPC, terpenes are adsorbed to the porous surface of the solid extractant whether by physisorption or chemisorption, forming a film in the porous surface of the extractant, without altering the terpene structure [31]. Eventually, factors such as the chemical structure of the adsorber, pore size, particle size, and specific surface area will affect the adsorption efficiency [32].…”

Section: Introductionmentioning

confidence: 99%

Improved Production and In Situ Recovery of Sesquiterpene (+)-Zizaene from Metabolically-Engineered E. coli

2019

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The sesquiterpene (+)-zizaene is the direct precursor of khusimol, the main fragrant compound of the vetiver essential oil from Chrysopogon zizanioides and used in nearly 20% of men’s fine perfumery. The biotechnological production of such fragrant sesquiterpenes is a promising alternative towards sustainability; nevertheless, product recovery from fermentation is one of the main constraints. In an effort to improve the (+)-zizaene recovery from a metabolically-engineered Escherichia coli, we developed an integrated bioprocess by coupling fermentation and (+)-zizaene recovery using adsorber extractants. Initially, (+)-zizaene volatilization was confirmed from cultivations with no extractants but application of liquid–liquid phase partitioning cultivation (LLPPC) improved (+)-zizaene recovery nearly 4-fold. Furthermore, solid–liquid phase partitioning cultivation (SLPPC) was evaluated by screening polymeric adsorbers, where Diaion HP20 reached the highest recovery. Bioprocess was scaled up to 2 L bioreactors and in situ recovery configurations integrated to fermentation were evaluated. External recovery configuration was performed with an expanded bed adsorption column and improved (+)-zizaene titers 2.5-fold higher than LLPPC. Moreover, internal recovery configuration (IRC) further enhanced the (+)-zizaene titers 2.2-fold, whereas adsorption velocity was determined as critical parameter for recovery efficiency. Consequently, IRC improved the (+)-zizaene titer 8.4-fold and productivity 3-fold from our last report, achieving a (+)-zizaene titer of 211.13 mg L−1 and productivity of 3.2 mg L−1 h−1. This study provides further knowledge for integration of terpene bioprocesses by in situ product recovery, which could be applied for many terpene studies towards the industrialization of fragrant molecules.

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Section: Introductionmentioning

confidence: 99%

Improved Production and In Situ Recovery of Sesquiterpene (+)-Zizaene from Metabolically-Engineered E. coli

2019

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“…Additionally, the team has outlined several goals for future research, including, but not limited to, improving the catalytic activity of TPS7, fine-tuning the expression levels of several inter-and intra-modules of the metabolic pathway, and optimizing the fermentation step in real time with online monitoring [19]. It is worth noting here that a new efficient chromatographic method for the purification of β-caryophyllene from E. coli supernatant has recently been developed [73]. It uses the polymeric material Rensa ® RP from Biotage, Uppsala, Sweden as an adsorbent and the green solvent ethanol as an eluent.…”

Section: E Colimentioning

confidence: 99%

An Update on Microbial Biosynthesis of β-Caryophyllene, a Sesquiterpene with Multi-Pharmacological Properties

Tsigoriyna,

Sango,

Batovska

2024

Fermentation

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The sesquiterpene β-caryophyllene (BCP) is a major component of various plant essential oils, to which it confers a unique spicy aroma. It is mainly used as a fragrance additive in the food, cosmetic and perfume industries, with an annual consumption ranging between 100 and 1000 metric tons worldwide. Recently, BCP has attracted attention as a promising precursor for the production of high-density fuels and for its various biological activities and pharmacological effects. These include antioxidant, anti-inflammatory, anticancer, immune–modulatory, and many other activities. Due to its underlying mechanisms, β-caryophyllene interacts with various human receptors, including CB2 of the endocannabinoid system, which defines it as a phytocannabinoid with therapeutic potential for certain serious conditions. Due to β-caryophyllene’s high utility, various green and sustainable strategies for its production in microorganisms have been developed. This article provides an update on the state-of-the-art in this field to identify directions for further development to extend the compound’s potential.

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“…The Fig. 1 shows some of the important phytochemicals found in guava leaves [Phenolic (gallic acid (8)), Flavonoid (quercetin (9)), Ellagitannins (corilagin (10)), Tannin (pedunculagin (11)) and Terpenoid (β-caryophyllene (12)) respectively.…”

Section: Phytochemistry Of P Guajava Leavesmentioning

confidence: 99%

Phytochemistry and medicinal properties of Psidium guajava L. leaves: A review

et al. 2021

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Psidium guajava L. (Myrtaceae), also known as guava, is a medicinal tree native to tropical America that has been introduced and is widely available in many countries. Almost all plant parts of P. guajava have a long history of being used to treat a variety of ailments, in addition to applications as foods. Guava leaves are used as both medicine and food purposes, and there are numerous scientific reports on their medicinal uses, chemical composition and pharmacological properties. Cancer, blood pressure, diarrhea, bowel irregularities, diabetes, cough, cold, constipation, dysentery, scurvy, weight loss, improves skins tonicity are some of the diseases treated with guava leaves. Polyphenols, flavonoids, saponins, tannins, terpenoids, glycosides, flavones, cardiac glycosides, cardenolides, phlobatanins, steroids and other classes of bioactive compounds have been identified from the leaves. The primary chemical constituents of guava leaves are phenolic compounds, iso-flavonoids, gallic acid, catechin, quercetin, epicathechin, rutin, naringenin, kaempferol, caryophyllene oxide, p-selinene etc. Several studies have demonstrated its pharmacological activities including antioxidant, antimicrobial, antidiabetic, antitumor, anticancer, antidiarrheal, healing, cytotoxic, hepatoprotective, anti-inflammatory, antimalarial/ anti-plasmodial, dental plaque, antiglycative and many more. This review is aimed on compiling all the literature reported on pharmacological activities and phytochemical compositions of guava leaves as a support to the scientific community for further studies and to provide scientific data to validate its traditional uses.

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One-Step Purification of Microbially Produced Hydrophobic Terpenes via Process Chromatography

Cited by 7 publications

References 64 publications

Improved Production and In Situ Recovery of Sesquiterpene (+)-Zizaene from Metabolically-Engineered E. coli

Improved Production and In Situ Recovery of Sesquiterpene (+)-Zizaene from Metabolically-Engineered E. coli

An Update on Microbial Biosynthesis of β-Caryophyllene, a Sesquiterpene with Multi-Pharmacological Properties

Phytochemistry and medicinal properties of Psidium guajava L. leaves: A review

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