One-step separation-free detection of carcinoembryonic antigen in whole serum: Combination of two-photon excitation fluorescence and optical trapping

Li, Chengyu; Cao, Di; Qi, Changxing; Chen, Honglei; Wan, Ya-Tao; Lin, Yi; Zhang, Zhiling; Pang, Dai‐Wen; Tang, Hong‐Wu

doi:10.1016/j.bios.2016.11.031

Cited by 16 publications

(7 citation statements)

References 29 publications

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“…To date, various analysis techniques have been developed for cardiac troponin detection, including the use of chemiluminescent immunoassays [ 4 ], enzyme-linked immunosorbent assays [ 5 ], electrical detections, and the use of aptamer-based biosensors [ 6 , 7 ]. In order to improve detection sensitivity and the limit of detection (LOD), researches were mainly focused on new strategies such as pulse laser technology [ 8 ], upconversion rare-earth probes, and surface plasmon resonance (SPR) technology [ 9 , 10 ]. Although those techniques are expected to pave the way for improved specificity and sensitivity, the demand for extremely expensive devices and cumbersome operation both greatly limits their feasibility.…”

Section: Introductionmentioning

confidence: 99%

Rapid and Sensitive Detection of Cardiac Troponin I for Point-of-Care Tests Based on Red Fluorescent Microspheres

Cai

Kang²,

et al. 2018

Molecules

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A reliable lateral flow immunoassay (LFIA) based on a facile one-step synthesis of single microspheres in combining with immunochromatography technique was developed to establish a new point-of-care test (POCT) for the rapid and early detection of cardiac troponin I (cTnI), a kind of cardiac specific biomarker for acute myocardial infarction (AMI). The double layered microspheres with clear core-shell structures were produced using soap-free emulsion polymerization method with inexpensive compounds (styrene and acrylic acid). The synthetic process was simple, rapid and easy to control due to one-step synthesis without any complicated procedures. The microspheres are nanostructure with high surface area, which have numerous carboxyl groups on the out layer, resulting in high-efficiency coupling between the carrier and antibody via amide bond. Meanwhile, the red fluorescent dye, Nile-red (NR), was wrapped inside the microspheres to improve its stability, as well to reduce the background noise, because of its higher emission wavelength than interference from real plasma samples. The core-shell structures provided different functional areas to separate antibody and dyes, so the immunoassay has highly sensitive, wide working curves in the range of 0–40 ng/mL, low limits of detection (LOD) at 0.016 ng/mL, and limits of quantification (LOQ) at 0.087 ng/mL with coefficient of variations (CV) of 10%. This strategy suggested an outstanding platform for LFIA, with good reproducibility and stability to straightforwardly analyze the plasma samples without washing steps, thereby reducing the operating procedures for non-professionals and promoting detection efficiency. The whole detection process can be completed in less than 15 min. This novel immunoassay offers a reliable and favorable analytical result by detecting the real samples, indicating that it holds great potential as a new alternative for biomolecule detection in complex samples, for the early detection of cardiac specific biomarkers.

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Section: Introductionmentioning

confidence: 99%

Rapid and Sensitive Detection of Cardiac Troponin I for Point-of-Care Tests Based on Red Fluorescent Microspheres

Cai

Kang²,

et al. 2018

Molecules

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“…41,42 The new sensing protocols are available to design a smart switch that the target intelligently triggers to open the pore and release biomolecules, which makes the signal depend on the host−guest interaction. 42,46 To address the mentioned challenges, we design a PADbased visual fluorescence protocol for monitoring carcinoembryonic antigen (CEA, as a model, one of the widely used biomarkers for clinic diagnosis and treatment of cancer 43 ) based on GOD-QD-impregnated paper and target-recognition cargo release from mesoporous silica (Scheme 1). A common centrifugal tube is chosen as the detection cell, while glass microfiber filter (paper) is used as the visual support.…”

mentioning

confidence: 99%

“…To address the mentioned challenges, we design a PAD-based visual fluorescence protocol for monitoring carcinoembryonic antigen (CEA, as a model, one of the widely used biomarkers for clinic diagnosis and treatment of cancer) based on GOD-QD-impregnated paper and target-recognition cargo release from mesoporous silica (Scheme ). A common centrifugal tube is chosen as the detection cell, while glass microfiber filter (paper) is used as the visual support.…”

mentioning

confidence: 99%

Bioresponsive Release System for Visual Fluorescence Detection of Carcinoembryonic Antigen from Mesoporous Silica Nanocontainers Mediated Optical Color on Quantum Dot-Enzyme-Impregnated Paper

2017

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An all-in-one paper-based analytical device (PAD) was successfully developed for visual fluorescence detection of carcinoembryonic antigen (CEA) on CdTe/CdSe quantum dot (QD)-enzyme-impregnated paper by coupling with a bioresponsive controlled-release system from DNA-gated mesoporous silica nanocontainers (MSNs). The assay was carried out in a centrifuge tube by using glucose-loaded MSNs with a CEA aptamer and a QD-enzyme-paper attached on the lid. Initially, single-strand complementary DNA to a CEA aptamer was covalently conjugated to the aminated MSN, and then glucose (enzyme substrate) molecules were gated into the pore with the help of the aptamer. Glucose oxidase (GOD) and CdTe/CdSe QDs were coimmobilized on paper for the visual fluorescence signal output. Upon target CEA introduction in the detection cell, the analyte specifically reacted with the immobilized aptamer on the MSN to open the pore, thereby resulting in the glucose release. The released glucose was oxidized by the immobilized GOD on paper to produce gluconic acid and hydrogen peroxide, and the latter quenched the fluorescence of CdTe/CdSe QDs, which could be determined by the naked eye on a portable smartphone and a commercial fluorospectrometer. Under optimal conditions, the PAD-based sensing system enabled sensitive discrimination of target CEA against other biomarkers or proteins in a linear range of 0.05-20 ng mL with a limit of detection of 6.7 pg mL (ppt). In addition, our strategy displayed high specificity, good reproducibility, and acceptable accuracy for analyzing human serum specimens with a commercial human CEA ELISA kit. Importantly, this methodology offers promise for simple analysis of biological samples and is suitable for use in the mass production of miniaturized devices, thus opening new opportunities for protein diagnostics and biosecurity.

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“…On the other hand, QDs have large two-photon cross-sectional efficiency, which has advantages of reducing background fluorescence and improving sensitivity in biodetection. By integrating the two-photon fluorescence of QDs with optical tweezers, Tang and co-workers developed sensitive separation-free methods to detect viral nucleotide sequences in complex samples. , The target sequences were enriched by polystyrene microspheres (PS) and labeled with QDs. The PS was then captured by near-infrared (NIR) optical tweezers to the center of the optical trap, where the nucleotide sequences could be quantitatively detected with a limit of 16 pM by determining the two-photon fluorescence of QDs (Figure B) .…”

Section: Qd-based Biodetectionmentioning

confidence: 99%

Quantum Dot Based Biotracking and Biodetection

Zhang

Xie

et al. 2018

Anal. Chem.

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One-step separation-free detection of carcinoembryonic antigen in whole serum: Combination of two-photon excitation fluorescence and optical trapping

Cited by 16 publications

References 29 publications

Rapid and Sensitive Detection of Cardiac Troponin I for Point-of-Care Tests Based on Red Fluorescent Microspheres

Rapid and Sensitive Detection of Cardiac Troponin I for Point-of-Care Tests Based on Red Fluorescent Microspheres

Bioresponsive Release System for Visual Fluorescence Detection of Carcinoembryonic Antigen from Mesoporous Silica Nanocontainers Mediated Optical Color on Quantum Dot-Enzyme-Impregnated Paper

Quantum Dot Based Biotracking and Biodetection

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