One-step simultaneous quantitative detection of three pesticides based on bimetallic organic framework nanomaterials and aptamers

Abstract

We have developed a new method of one-step simultaneous detection for three pesticides including acetamiprid, atrazine and carbendazim based on organic framework nanomaterial Cu/UiO-66 and three different fluorescent dyes-labeled pesticide aptamers.Cu/UiO-66 can be easily combined with pesticide aptamers through strong coordination, then aptamers were adsorbed to the surface of Cu/UiO-66, which brings the dyes and Cu/UiO-66 into close proximity. And then, the fluorescence of dyes were quenched by Cu/UiO-66. When the target pesticides appeared, the aptamers reacted with corresponding target pesticides and formed special spatial structure, then the dyes were far away from the surface of Cu/UiO-66 and the fluorescence of dyes is resumed. Thus, the one-step simultaneous detection for three pesticides can be achieved by synchronous fluorescence analysis. The detection limit of acetamiprid, atrazine and carbendazim were 0.1 nmol/L, 1.6 nmol/L and 0.3 nmol/L, respectively. This method has a good sensitivity, low detection limit and high selectivity.

Photoinduced Electron Transfer-Triggered g-C3N4\Rhodamine B Sensing System for the Ratiometric Fluorescence Quantitation of Carbendazim

“…At present, several kinds of aptamers are used as fluorescence biosensors to quantitatively detect carbendazim. − Shi et al developed a fluorometric assay for carbendazim determination in aqueous samples based on an unmodified Car-specific aptamer and the fluorescent intercalator SYBR Green I . A multiplexed biosensor based on fluorescence resonance energy transfer (FRET) from upconversion nanoparticles to single black phosphorus nanosheets was successfully developed by Wang et al for the detection of paraquat and carbendazim .…”