One-step synthesis of gene carrier via gamma irradiation and its application in tumor gene therapy

Jeong, Sunho; Park, Seong-Cheol; Park, Sun-Jeong; Kim, Young Ho; Heo, Heeok; Park, Jong‐Seok; Gwon, Hui-Jeong; Lim, Youn-Mook; Jang, Mi-Kyeong

doi:10.2147/ijn.s149532

Cited by 8 publications

(4 citation statements)

References 39 publications

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“…The higher gamma irradiation increased the breaking of the covalent bonds in the structure of the silicone films. In consequence, there was a higher radical formation to graft 2MBA [ 30 ]. The increasing monomer concentrations experiment demonstrated a growth in the graft percentage in the intervals between 0.4 and 1.5 M ( Figure 1 C).…”

Section: Resultsmentioning

confidence: 99%

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Radiation Grafting of a Polymeric Prodrug onto Silicone Rubber for Potential Medical/Surgical Procedures

et al. 2020

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Silicone rubber (SR) is a material used for medical procedures, with a common example of its application being in implants for cosmetic or plastic surgeries. It is also an essential component for the development of medical devices. SR was functionalized with the polymeric prodrug of poly(2-methacryloyloxy-benzoic acid) (poly(2MBA)) to render the analgesic anti-inflammatory drug salicylic acid by hydrolysis. The system was designed by functionalizing SR films (0.5 cm × 1 cm) with a direct grafting method, using gamma irradiation (60Co source) to induce the polymerization process. The absorbed dose (from 20 to 100 kGy) and the monomer concentration (between 0.4 and 1.5 M) were critical in controlling the surface and the bulk modifications of SR. Grafting poly(2MBA) onto SR (SR-g-2MBA) were characterized by attenuated total reflectance Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy/energy-dispersive X-ray spectrometry, fluorescence microscopy, the contact angle, and the swelling. SR-g-2MBA demonstrated the drug’s sustained and pH-dependent release in simulated physiological mediums (pH = 5.5 and 7.4). The drug’s release was quantified by high-performance liquid chromatography and confirmed by gas chromatography–mass spectrometry. Finally, cytocompatibility was demonstrated in murine fibroblast and human cervical cancer cell lines. The developed systems provide new polymeric drug release systems for medical silicone applications.

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Section: Resultsmentioning

confidence: 99%

“…there was a higher radical formation to graft 2MBA [30]. The increasing monomer concentrations experiment demonstrated a growth in the graft percentage in the intervals between 0.4 and 1.5 M ( Figure 1C).…”

Section: Synthesis Of Sr-g-2mbamentioning

confidence: 86%

Radiation Grafting of a Polymeric Prodrug onto Silicone Rubber for Potential Medical/Surgical Procedures

et al. 2020

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“…The results showed the high potential of the pectin-co-poly(MAA) hydrogels for the targeted delivery of 5-FU with negligible exposure of the upper gastrointestinal tract. Sung In Jeong et al studied the one-step synthesis of a gene carrier via gamma irradiation and its application in tumor gene therapy [ 122 ]. They revealed the selective grafting of AEMA onto C6-OH groups of WSC.…”

Section: Brief Application Of Hydrogelsmentioning

confidence: 99%

A Comprehensive Review of Radiation-Induced Hydrogels: Synthesis, Properties, and Multidimensional Applications

Ahmed,

Islam,

Hasan

et al. 2024

Gels

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At the forefront of advanced material technology, radiation-induced hydrogels present a promising avenue for innovation across various sectors, utilizing gamma radiation, electron beam radiation, and UV radiation. Through the unique synthesis process involving radiation exposure, these hydrogels exhibit exceptional properties that make them highly versatile and valuable for a multitude of applications. This paper focuses on the intricacies of the synthesis methods employed in creating these radiation-induced hydrogels, shedding light on their structural characteristics and functional benefits. In particular, the paper analyzes the diverse utility of these hydrogels in biomedicine and agriculture, showcasing their potential for applications such as targeted drug delivery, injury recovery, and even environmental engineering solutions. By analyzing current research trends and highlighting potential future directions, this review aims to underscore the transformative impact that radiation-induced hydrogels could have on various industries and the advancement of biomedical and agricultural practices.

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“…In a previous study, we grafted electrospun PCL nanofibers with amino groups of 2-aminoethyl methacrylate (AEMA) using gamma irradiation [ 17 ]. Gamma irradiation induces graft polymerization, where the associated energy uniformly free-radicalizes the reaction between acrylate monomers and polymers [ 18 , 19 , 20 ]. In this study, to overcome the disadvantages of small-diameter vascular grafts, we developed synthetic vascular grafts with electrospun, tubular, surface-modified PCL nanofibers.…”

Section: Introductionmentioning

confidence: 99%

In Vivo Evaluation of Gamma-Irradiated and Heparin-Immobilized Small-Diameter Polycaprolactone Vascular Grafts with VEGF in Aged Rats

et al. 2022

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The effectiveness of small-diameter vascular grafts depends on their antithrombogenic properties and ability to undergo accelerated endothelialization. The extreme hydrophobic nature of poly(ε-caprolactone) (PCL) hinders vascular tissue integration, limiting its use in medical implants. To enhance the antithrombogenicity of PCL as a biomaterial, we grafted 2-aminoethyl methacrylate (AEMA) hydrochloride onto the PCL surface using gamma irradiation; developed a biodegradable heparin-immobilized PCL nanofibrous scaffold using gamma irradiation and N-(3-dimethylaminopropyl)-N′-ethyl carbodiimide hydrochloride/N-hydroxysuccinimide reaction chemistry; and incorporated vascular endothelial growth factor (VEGF) into the scaffold to promote vascular endothelial cell proliferation and prevent thrombosis on the vascular grafts. We assessed the physicochemical properties of PCL, heparin-AEMA-PCL (H-PCL), and VEGF-loaded heparin-AEMA-PCL (VH-PCL) vascular grafts using scanning electron microscopy, attenuated total reflection–Fourier transform infrared spectroscopy, toluidine blue O staining, and fibrinogen adsorption and surface wettability measurement. In addition, we implanted the vascular grafts into 24-month-old Sprague Dawley rats and evaluated them for 3 months. The H-PCL and VH-PCL vascular grafts improved the recovery of blood vessel function by promoting the proliferation of endothelial cells and preventing thrombosis in clinical and histological evaluation, indicating their potential to serve as functional vascular grafts in vascular tissue engineering.

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One-step synthesis of gene carrier via gamma irradiation and its application in tumor gene therapy

Cited by 8 publications

References 39 publications

Radiation Grafting of a Polymeric Prodrug onto Silicone Rubber for Potential Medical/Surgical Procedures

Radiation Grafting of a Polymeric Prodrug onto Silicone Rubber for Potential Medical/Surgical Procedures

A Comprehensive Review of Radiation-Induced Hydrogels: Synthesis, Properties, and Multidimensional Applications

In Vivo Evaluation of Gamma-Irradiated and Heparin-Immobilized Small-Diameter Polycaprolactone Vascular Grafts with VEGF in Aged Rats

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