One‐step triplex high‐resolution melting (HRM) analysis for rapid identification of Atlantic cod (Gadus morhua), Alaska pollock (Gadus chalcogrammus) and haddock (Melanogrammus aeglefinus)

Abstract

Specific primers were designed for Gadus morhua, Gadus chalcogrammus and Melanogrammus aeglefinus. Specificity of these specific primers was confirmed. Triplex HRM assay allowed easy and quick differentiation of the three species. G. morhua was detected in 30.8% (4/13) commercial codfish products. G. chalcogrammus was detected in 38.5% (5/13) commercial codfish products.

Closed‐tube visual detection of Atlantic cod (Gadus morhua) using self‐quenched primer coupled with a designed loop‐mediated isothermal amplification vessel

“…However, due to declining stocks in most source areas of the world, indistinguishable features for processed products, and poor transparency in the global supply chain, species adulteration frequently occurs with Atlantic cod 2‐4 . Moreover, a great variety of species have been identified in Atlantic cod products, including Pacific cod ( G. macrocephalus ), 2 Alaska pollock ( Theragra chalcogramma ), 5 saithe ( Pollachius virens ), 6 European hake ( Merluccius merluccius ), 7 Greenland halibut ( Reinhardtius hippoglossoides ), 3 anglerfish ( Lophius litulon ), 8 oilfish ( Ruvettus pretiosus , Lepidocybium flavobrunneum ) 9 and even pufferfish ( Lagochephalus spp.) 10 .…”

“…Fish species authentication can be achieved using several analytical methods, with DNA‐based technique the most widely used one 12 . In particular, various polymerase chain reaction (PCR) methods have been developed for accurate identification of Atlantic cod, such as real‐time PCR, 13 multiplex PCR 8 and DNA barcoding 10 . However, a key disadvantage of PCR‐based methods is the necessity for expensive equipment and long amplification time, greatly restricting the applications for on‐site or rapid testing.…”

“…While CF-13 and CF-15-CF-21 are processed in China, country of origin is not mandatory according to Chinese regulation. Atlantic cod-specific PCR was conducted according to the protocol of Chen et al8 DNA barcoding was conducted according to the protocol of Xiong et al10 www.soci.org X Hanyue et al…”

Visual detection of rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar) simultaneously by duplex loop-mediated isothermal amplification

“…In particular, nucleic acid amplification tests (NAATs) have become an indispensable tool, where a few copies of DNA fragments can be exponentially amplified by the use of a thermal cycler since the revolutionary development of the polymerase chain reaction (PCR). Moreover, in the course of the last 10 years, a great variety of PCR-based analytical methods have been applied for fish authenticity studies ( Chen et al, 2021 , Kotsanopoulos et al, 2021 , Shi et al, 2020 ). Nevertheless, these PCR-based methods generally require expensive equipments and are time-consuming to perform, precluding the facile implementation of these techniques for on-site or rapid testing.…”