One-step UV lithography for activation of inert hydrocarbon monolayers and preparation of protein micropatterns

Xue, Changying; Yang, Kun‐Lin

doi:10.1016/j.jcis.2009.12.015

Cited by 20 publications

(9 citation statements)

References 48 publications

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“…To overcome the problem, some researchers have reported the use of UV-absorbing molecules such as 1-(2-nitrophenyl)-ethyl-5-trichlorosilylpentanoate [ 18 ] or triphenylsulfonium triflate [ 19 ] that can activate surface UV reactions. Xue and co-workers invented the use of a minimal UV exposure dosage (~1.35 J/cm 2 ) under ambient conditions without using a high-vacuum system [ 20 ]. Alonso et al studied site-selective immobilization of protein in which photoreactive groups such as the benzylthiocyanate group and trimethoxysilyl group were self-assembled on a silicon oxide surface.…”

Section: Introductionmentioning

confidence: 99%

Patterned Array of Poly(ethylene glycol) Silane Monolayer for Label-Free Detection of Dengue

Rosly

Ahmad

Abdullah

et al. 2016

Sensors

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In the present study, the construction of arrays on silicon for naked-eye detection of DNA dengue was demonstrated. The array was created by exposing a polyethylene glycol (PEG) silane monolayer to 254 nm ultraviolet (UV) light through a photomask. Formation of the PEG silane monolayer and photomodifed surface properties was thoroughly characterized by using atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), and contact angle measurements. The results of XPS confirmed that irradiation of ultraviolet (UV) light generates an aldehyde functional group that offers conjugation sites of amino DNA probe for detection of a specific dengue virus target DNA. Employing a gold enhancement process after inducing the electrostatic interaction between positively charged gold nanoparticles and the negatively charged target DNA hybridized to the DNA capture probe allowed to visualize the array with naked eye. The developed arrays demonstrated excellent performance in diagnosis of dengue with a detection limit as low as 10 pM. The selectivity of DNA arrays was also examined using a single base mismatch and noncomplementary target DNA.

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Section: Introductionmentioning

confidence: 99%

Patterned Array of Poly(ethylene glycol) Silane Monolayer for Label-Free Detection of Dengue

Rosly

Ahmad

Abdullah

et al. 2016

Sensors

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show abstract

“…Apparently, UV treatment contributed to this result. In the literature, it has been reported that UV treatment on a DMOAP surface can lead to generation of some functional groups such as alcohol, aldehyde and carboxylic acid on the surface [26]. One possible reason for the bright spots in Fig.…”

Section: Effect Of Sodium Chloride On Orientations Of Lcsmentioning

confidence: 98%

Applications of metal ions and liquid crystals for multiplex detection of DNA

Liu

Yang

2015

Journal of Colloid and Interface Science

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“…Therefore, we believe that the transfer of protein from untreated PDMS to DMOAP-coated glass slide is because the hydrophobic interaction between proteins and DMOAP is stronger than that between protein and PDMS. After the UV treatment, however, some reactive functional groups such as aldehyde may form on the surface of PDMS as suggested by Xue et al [33]. The aldehyde group is known to form covalent bonds with the free amine groups of protein, and prevents the transfer of protein during lCP.…”

Section: Effect Of Uv Exposure Time On Lcpmentioning

confidence: 99%