One strain-many compounds (OSMAC) method for production of polyketides, azaphilones, and an isochromanone using the endophytic fungus Dothideomycete sp.

Hewage, Ranuka T.; Aree, Thammarat; Mahidol, Chulabhorn; Ruchirawat, Somsak; Kittakoop, Prasat

doi:10.1016/j.phytochem.2014.09.013

Cited by 110 publications

(78 citation statements)

References 28 publications

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“…The OSMAC approach is a prominent strategy to probe the importance of fungal fermentation conditions, potentially generating broader chemical diversity [29, 30] and/or targeting the enhanced production of a distinct class of secondary metabolites. In this study, several fungal strains were selected from the Mycosynthetix library.…”

Section: Introductionmentioning

confidence: 99%

Media studies to enhance the production of verticillins facilitated by in situ chemical analysis

Amrine

Raja

Darveaux

et al. 2018

Journal of Industrial Microbiology and Biotechnology

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Verticillins are a group of epipolythiodioxopiperazine alkaloids that have displayed potent cytotoxicity. To evaluate their potential further, a larger supply of these compounds was needed for both in vivo studies and analogue development via semisynthesis. To optimize the biosynthesis of these secondary metabolites, their production was analyzed in two different fungal strains (MSX59553 and MSX79542) under a suite of fermentation conditions. These studies were facilitated by the use of the droplet-liquid microjunction-surface sampling probe (droplet probe), which enables chemical analysis in situ directly from the surface of the cultures. These experiments showed that the production of verticillins was greatly affected by growth conditions; a significantly higher quantity of these alkaloids was noted when the fungal strains were grown on an oatmeal-based medium. Using these technologies to select the best among the tested growth conditions, the production of the verticillin analogues was increased while concomitantly decreasing the time required for fermentations from 5 weeks to about 11 days. Importantly, where we could previously supply 5–10 mg every 6 weeks, we are now able to supply 50–150 mg quantities of key analogues per month via laboratory scale fermentation.

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Section: Introductionmentioning

confidence: 99%

Media studies to enhance the production of verticillins facilitated by in situ chemical analysis

Amrine

Raja

Darveaux

et al. 2018

Journal of Industrial Microbiology and Biotechnology

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“…The aim of this study was to enhance the antibiotics and secondary metabolites detection from strain KBS50 using the OSMAC fermentation approach. The fermentation strategy has been used successfully to increase secondary metabolites production in other actinomycetes and fungi (Bode et al, 2002;Bills et al, 2008;Hewage et al, 2014). As stated by Bode et al (2002), the OSMAC is "a random approach" whereby the cultivation parameters for a single strain can be altered in many possible ways that may lead to the discovery of new secondary metabolites.…”

Section: Discussionmentioning

confidence: 99%

“…The present study aimed to further evaluate the secondary metabolites biosynthetic capability of strain KBS50 using the One Strain Many Compound (OSMAC) method. The OSMAC approach has been used to unselectively enhance the production of compounds from microbial strains through manipulation of various cultivation parameters (Bode et al, 2002;Martín, 2004;Bills et al, 2008;Hewage et al, 2014). This strategy provides nutritional and/or physical variation between different cultivation conditions which could potentially activate or increase the expression of biosynthetic genes in the strain's genome.…”

Section: Introductionmentioning

confidence: 99%

Enhanced detection of antimicrobial activities and secondary metabolites production from Plantactinospora sp. KBS50 cultivated using the OSMAC fermentation approach

Juboi¹,

Hwang²,

Yeo³

et al. 2019

MJM

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Aims:A rare marine-derived actinomycete, Plantactinospora sp. KBS50, has been identified as a potential source of bioactive secondary metabolites compounds. The present study aimed to evaluate the secondary metabolites biosynthetic capability of strain KBS50 using the One Strain Many Compound (OSMAC) fermentation strategy. Methodology and results: Strain KBS50 was fermented in a basal medium (ISP2) supplemented with selected biological and chemical elicitors, as well as cultivation at different pH value and incubation temperature. Statistical analysis revealed that the antimicrobial activities were significantly increased, as compared to the basal medium, ISP2. Similarly, the comparative High Performance Liquid Chromatography (HPLC) analysis showed an increase in secondary metabolites production, as well as the detection of potential new metabolites, particularly from the crude extracts of ISP2 medium supplemented with 1% (w/v) sodium chloride and with the culture filtrate of Aspergillus niger. The bioassayguided fractionation showed that the extract of strain KBS50 contains multiple compounds with antibacterial activity against the Gram-positive strains. Further fractionation led to the isolation of two semi-pure compounds (compound 3 and 4) with bactericidal properties against Staphylococcus aureus. The Minimum Inhibitory Concentration (MIC) values of compound 3 and 4 were recorded at 7.81 µg/mL and 62.50 µg/mL, respectively. The minimum bactericidal concentration (MBC) for compound 3 was recorded at 15.63 µg/mL while the MBC for compound 4 was recorded as 125.00 µg/mL. Conclusion, significance and impact of study: The OSMAC fermentation strategy used in this study had successfully enhanced the detection of antibiotics and secondary metabolites from Plantactinospora sp. KBS50. The bioassay-guided fractionation further established the capability of strain KBS50 as a source of bioactive secondary metabolite compounds with potent antimicrobial activity.

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“…CRI7 by changing the medium type (PDB vs. Czapek malt medium) as well as by using different nutrient sources (potato for PDB medium and malt extract for Czapek malt medium) resulted in distinct secondary metabolite production of this fungus. 21,22 Interestingly, a series of studies have reported the isolation of halogenated natural products from fungal cultures grown on media to which different halide salts had been added, thus highlighting the fungal capability to utilize different halogen sources when present in the media. [23][24][25][26] In addition, supplementing media with trace elements, e.g.…”

Section: Introductionmentioning

confidence: 99%

Expanding the chemical diversity of an endophytic fungusBulgaria inquinans, an ascomycete associated with mistletoe, through an OSMAC approach

et al. 2019

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One strain-many compounds (OSMAC) method for production of polyketides, azaphilones, and an isochromanone using the endophytic fungus Dothideomycete sp.

Cited by 110 publications

References 28 publications

Media studies to enhance the production of verticillins facilitated by in situ chemical analysis

Media studies to enhance the production of verticillins facilitated by in situ chemical analysis

Enhanced detection of antimicrobial activities and secondary metabolites production from Plantactinospora sp. KBS50 cultivated using the OSMAC fermentation approach

Expanding the chemical diversity of an endophytic fungusBulgaria inquinans, an ascomycete associated with mistletoe, through an OSMAC approach

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