2019
DOI: 10.1021/acs.analchem.9b03492
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One-to-Many Single Entity Electrochemistry Biosensing for Ultrasensitive Detection of microRNA

Abstract: Single-entity electrochemistry (SEEC), a promising method for biosensing, has an intrinsic limitation on sensitivity since at most one colliding entity can be successfully triggered by one target. Here, we take advantage of one-to-many (1:n) signal amplification to develop a new single-entity electrochemistry biosensing (SEECBS), integrating satellite magnetic nanoparticle (MN)-DNA-Pt nanoparticle (NP) conjugates, duplex-specific nuclease (DSN) assisted Pt NPs releasing with stabilization, and effective collis… Show more

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Cited by 53 publications
(42 citation statements)
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“…Generally, miRNAs are believed to function through complete or incomplete complementary binding with the 3'UTRs of target genes, and a network of miRNAs directly or indirectly controls the expression of target genes in a one-to-many or many-to-one manner (32). Our results identified 123 predicted target genes of miR-125a-5p, miR-301a-5p, miR-132-5p, and miR-146b-3p for further study.…”
Section: Discussionmentioning
confidence: 75%
“…Generally, miRNAs are believed to function through complete or incomplete complementary binding with the 3'UTRs of target genes, and a network of miRNAs directly or indirectly controls the expression of target genes in a one-to-many or many-to-one manner (32). Our results identified 123 predicted target genes of miR-125a-5p, miR-301a-5p, miR-132-5p, and miR-146b-3p for further study.…”
Section: Discussionmentioning
confidence: 75%
“…Crooks et al used Pt NPs modified with capture probes to achieve specific detection of microRNA with a detection limit of 100 pM ( Castaneda et al, 2017 ). Bai et al applied the similar method and combined duplex-specific nuclease-mediated cycle amplification to achieve more sensitive detection of miRNA-21 ( Bai et al, 2020 ). Signal amplification strategies such as hybridization chain reaction, chain replacement, enzyme-catalyzed reaction have been very mature for DNA/RNA detection ( Castaneda et al, 2017 ; Wang et al, 2021a , Wang et al, 2021b ).…”
Section: Introductionmentioning
confidence: 99%
“…Therefore, in recent years, the hybridization and strand displacement amplification strategy has received extensive attention 7 due its advantage of enzymefree reactions. Fluorescence, [8][9][10][11][12] electrochemistry, [13][14][15][16] colorimetry, 17 and surface-enhanced Raman spectroscopy (SERS) 18,19 are several common strategies for manufacturing DNA fragment sensors for DNA analysis. Among these methods, the fluorescence method is highly sensitive and easy to read.…”
Section: Introductionmentioning
confidence: 99%