Online regeneration of a piezoelectric and impedimetric immunosensor for the detection of C-reactive protein on the oriented antibody gold surface

Adesina, Abiola Olanike; Adeniyi, Omotayo; Mashazi, Philani

doi:10.1039/d2ay01245f

Cited by 4 publications

(1 citation statement)

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“…The detection limit was 0.58 fg/mL. Adesina et al [211] used a self-assembled monolayer (SAM) of 4-mercaptophenylboronic acid (4-MPBA) to orient immobilized anti-C-reactive protein (CRP) antibodies. Controlling ligand orientation was key to eliminating false positives and negatives during sample analysis.…”

Section: Impedimetric Detection Of Proteins Using Antibodiesmentioning

confidence: 99%

Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Sitkov,

Ryabko,

Moshnikov

et al. 2024

Micromachines

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Impedimetric biosensors represent a powerful and promising tool for studying and monitoring biological processes associated with proteins and can contribute to the development of new approaches in the diagnosis and treatment of diseases. The basic principles, analytical methods, and applications of hybrid impedimetric biosensors for express protein detection in biological fluids are described. The advantages of this type of biosensors, such as simplicity and speed of operation, sensitivity and selectivity of analysis, cost-effectiveness, and an ability to be integrated into hybrid microfluidic systems, are demonstrated. Current challenges and development prospects in this area are analyzed. They include (a) the selection of materials for electrodes and formation of nanostructures on their surface; (b) the development of efficient methods for biorecognition elements’ deposition on the electrodes’ surface, providing the specificity and sensitivity of biosensing; (c) the reducing of nonspecific binding and interference, which could affect specificity; (d) adapting biosensors to real samples and conditions of operation; (e) expanding the range of detected proteins; and, finally, (f) the development of biosensor integration into large microanalytical system technologies. This review could be useful for researchers working in the field of impedimetric biosensors for protein detection, as well as for those interested in the application of this type of biosensor in biomedical diagnostics.

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Section: Impedimetric Detection Of Proteins Using Antibodiesmentioning

confidence: 99%