Ontogenic changes in enzymes of carbon metabolism in relation to carbohydrate status in developing mungbean reproductive structures

Chopra, Jyoti; Kaur, Narinder; Gupta, Anil Kumar

doi:10.1016/s0031-9422(99)00545-2

Cited by 28 publications

(32 citation statements)

References 32 publications

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“…2) indicates the partitioning of sucrose into RFOs during this time of development. This data is supported by the similar trend of sucrose, raffinose and stachyose during seed development in pea, lupin, mungbean and lentil seeds (Chopra et al 2000;Chopra et al 2003;Pinheiro et al 2005). Rapid accumulation of phytic acid occurred from 28 to 35 DAF in seeds after RFOs accumulation though it remained depositing till 42 DAF (Fig.…”

Section: Discussionsupporting

confidence: 72%

Phytic acid and raffinose series oligosaccharides metabolism in developing chickpea seeds

Zhawar

Kaur

Gupta

2011

Physiol Mol Biol Plants

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Phytic acid and raffinose series oligosaccharides (RFOs) have anti-nutritional properties where phytic acid chelates minerals and reduces their bioavailability to humans and other animals, and RFOs cause flatulence. Both phytic acid and RFOs cannot be digested by monogastric animals and are released as pollutant-wastes. Efforts are being made to reduce the contents of these factors without affecting the viability of seeds. This will require a thorough understanding of their metabolism in different crops. Biosynthetic pathways of both metabolites though are interlinked but not well described. This study was made on metabolism of these two contents in developing chickpea (Cicer arietinum L cv GL 769) seeds. In this study, deposition of RFOs was found to occur before deposition of phytic acid. A decline in inorganic phosphorus and increase in phospholipid phosphorus and phytic acid was observed in seeds during development. Acid phosphatase was the major phosphatase in seed as well as podwall and its activity was highest at early stage of development, thereafter it decreased. Partitioning of 14 C label from 14 Cglucose and 14 C-sucrose into RFOs and phytic acid was studied in seeds in presence of inositol, galactose and iositol and galactose, which favored the view that galactinol synthase is not the key enzyme in RFOs synthesis.

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Section: Discussionsupporting

confidence: 72%

Phytic acid and raffinose series oligosaccharides metabolism in developing chickpea seeds

Zhawar

Kaur

Gupta

2011

Physiol Mol Biol Plants

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“…Enzyme extraction procedures were performed at 4°C. The sucrose metabolizing enzymes, alkaline invertase and sucrose synthase, were extracted and assayed by the methods described earlier (Kaur et al 2002b;Chopra et al 2000).…”

Section: Methodsmentioning

confidence: 99%

Effect of hydro- and osmopriming of chickpea (Cicer arietinum L.) seeds on enzymes of sucrose and nitrogen metabolism in nodules

2006

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Three year data on the effect of waterand mannitol (4%) priming of chickpea seeds (12 h at 25°C) showed higher number and biomass of nodules in the plants from primed seeds than from non-primed seeds. The biomass of nodules increased to 75 DAS but decreased by 90 DAS. Activities of sucrose metabolism enzymes (sucrose synthase (SS) and alkaline invertase) and of nitrogen metabolism (glutamine synthetase (GS), glutamate synthase (GOGAT) and glutamate dehydrogenase (GDH)) in nodules of primed and non-primed crops during development are reported. SS and alkaline invertase activities increased to 70 DAS and then decreased. In primed plants, the higher SS activity in nodules at 60 and 70 DAS might be responsible for providing more energy and carbon skeleton for nitrogen fixation and for ammonium assimilation in primed plants. At 85 DAS, though the SS activity decreased in comparison with the earlier growth stages, it was still higher in nodules of the primed crops than the non-primed crop. Activity of alkaline invertase was maximum at 70 DAS in the nodules of primed and non-primed crops. Priming increased nodule GS activity at 70 and 85 DAS. GOGAT activity was unaffected by priming but GDH activity was greater in nodules from primed crops at 50 DAS. Elevated SS and GS nodule activities in primed chickpeas might be responsible in increasing nodule biomass and metabolic activity thereby increasing seed fill.

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“…β-amylase was assayed essentially according to Duffus and Rosie (1973), using starch as substrate. Sucrose synthase was extracted as described previously and assayed by employing the method described in detail earlier by Chopra et al (2000). Conditions for linear rate with respect to time and substrate concentrations were determined for all enzymes in preliminary assays.…”

Section: Methodsmentioning

confidence: 99%

“…Genetic and biochemical data indicate a correlation between SuSy activity and sink strength in maize endosperm and potato tuber (Zrenner et al 1995, Sharma et al 1998. We have observed in mung bean and lentil seeds that SuSy activity controls the seed sink strength (Chopra et al 2000(Chopra et al , 2003. Hence, the rate of biomass accumulation and activities of AGPase, UGPase and SuSy enzymes with respect to the seed sink strength of mung bean constituted by 50 -60 % of starch biomass were compared.…”

Section: Introductionmentioning

confidence: 99%

Role of enzymes of sucrose-starch conversion in seed sink strength in mung bean

Chopra¹,

Kaur²,

Gupta³

2005

Biologia plant.

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Changes in the activities of sucrose synthase (SuSy), ADP-glucose pyrophosphorylase (AGPase), UDP-glucose pyrophosphorylase (UGPase), alkaline inorganic pyrophosphatase, 3-phosphoglycerate (3-PGA) phosphatase and amylases were monitored in relation to accumulation of starch in developing pods of mung bean (Vigna radiata L.). With the advancement in the seed development, the contents of starch rose with a concomitant fall in the branch of inflorescence and podwall after 10 d after flowering. The activity of UDPase in all the three pod tissues remained higher than the activity of AGPase showing it to be an important enzyme controlling carbon flux. The activity of alkaline inorganic pyrophosphatase in developing seed in contrast to 3-PGA phosphatase correlated with starch accumulation rate. Activity of β-amylase increased in all the pod tissues till maturity. It appears that the cooperative action of SuSy, UGPase and AGPase controls the efficient partitioning of sucrose into ADP glucose and thereby regulate the seed sink strength of the mung bean.Additional key words: alkaline inorganic pyrophosphatase, amylase, 3-PGA phosphatase, starch metabolism, sucrose synthase, Vigna radiata.

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Ontogenic changes in enzymes of carbon metabolism in relation to carbohydrate status in developing mungbean reproductive structures

Cited by 28 publications

References 32 publications

Phytic acid and raffinose series oligosaccharides metabolism in developing chickpea seeds

Phytic acid and raffinose series oligosaccharides metabolism in developing chickpea seeds

Effect of hydro- and osmopriming of chickpea (Cicer arietinum L.) seeds on enzymes of sucrose and nitrogen metabolism in nodules

Role of enzymes of sucrose-starch conversion in seed sink strength in mung bean

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