Ontogeny and distribution of alkaline and acid phosphatases in the digestive system of California halibut larvae (Paralichthys californicus)

Abstract

Studies aimed to assess the digestive physiology of marine fish larvae under culture conditions are important to further understand the functional characteristics and digestive capacities of the developing larvae. Most studies to date concentrate on intestinal lumen digestion and little attention to the absorption process. Thus, the objectives of this study were to histochemically detect and quantify some of the enzymes responsible for absorption and intracellular digestion of nutrients in the anterior and posterior intestine of California halibut larvae. Alkaline and acid phosphatases were detected from the first days post-hatch (dph). Alkaline phosphatase maintained a high level of activity during the first 20 dph in both intestinal regions. Thereafter, a clear intestinal regionalization of the activity was observed with the highest levels occurring in the anterior intestine. Acid phosphatase activity gradually increased in both intestinal regions during development, and a regionalization of the activity was not observed until late in development, once the ocular migration began. Highest levels were observed in the anterior intestine at the end of metamorphosis concomitant with the stomach development. The results from this study show some morphological and physiological changes are occurring during larval development and a clear regionalization of the absorption process as the larvae develops. These ontological changes must be considered in the elaboration of diets according to the digestive capacity of the larvae.

Replacement of fish oil with a DHA-richSchizochytriummeal on growth performance, activities of digestive enzyme and fatty acid profile of Pacific white shrimp (Litopenaeus vannamei) larvae

“…Digestion and absorption of nutrients depend on the activity of digestive enzymes of the brush border of the intestine (e.g. alkaline phosphatase and leucine aminopeptidase), which are responsible for the final stages of breaking down and assimilation of the food (Hassanatabar, Ouraji, Esmaeili, & Babaei, ; Zacarias‐Soto, Baron‐Sevilla, & Lazo, ). Brush border membrane (BBM) enzyme assays have been successfully used to determine the degree of the maturation process of the digestive function in intestine in fish larvae (Cahu & Zambonino‐Infante, ; Ma et al., ).…”

Histological structure of the digestive tract and digestive enzymatic activity of juvenile Pacific seahorse (Hippocampus ingens)

“…Additionally, the activity of enzymes found in the intestinal mucosa (acid and alkaline phosphatases, aminopeptidase) suggests the presence of fully functional enterocytes in the intestines. Indeed, acid and alkaline phosphatase has been associated with the maturation of the enterocytes of the digestive tract and the complete development of the absorptive function in the intestinal epithelium (He et al 2012, Zacarías-Soto et al 2013.…”

Vii Foro Iberoamericano De Los Recursos Marinos Y La Acuicultura

“…El hálibut P. olivaceus, es apetecido por su carne utilizada como sashimi en la comida oriental (Pham et al, 2014). En P. californicus, se han reportado bajas supervivencias en el cultivo larval, especialmente durante la deshabituación del alimento vivo (Zacarías- Soto et al, 2013). A pesar de su lento crecimiento, esta especie es atractiva para el desarrollo de la acuicultura marina artesanal.…”

“…(Zacarias- Soto et al, 2013), palometa Seriola lalandi (Chen et al, 2006), y cabrilla arenera Paralabrax maculatofasciatus (Peña et al, 2003). Desde la eclosión hasta los 5 DDE, las larvas de Cojinoba del Norte dependían exclusivamente de una fuente endógena de alimentación.…”