Ontogeny of Langerin/CD207 Expression in the Epidermis of Mice

Tripp, Christoph H.; Chang-Rodriguez, Souyet; Stoitzner, Patrizia; Holzmann, Sandra; Stössel, Hella; Douillard, Patrice; Saeland, Sem; Koch, Franz; Elbe‐Bürger, Adelheid; Romani, Nikolaus

doi:10.1111/j.0022-202x.2004.22337.x

Cited by 60 publications

(62 citation statements)

References 10 publications

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“…An epidermal MHC class II high dendritic network was observed in langerin Ϫ/Ϫ mice, indistinguishable from that of C57BL/6 control mice. Our results indicate that langerin is not necessary for the establishment of the LC system in the epidermis and support a recent description that, even though some MHC class II-positive cells are present in the epidermis of newborn mice, langerin expression is detectable only 2 to 3 days after birth (67). The phenotype of freshly isolated epidermal LC from langerin Ϫ/Ϫ mice was further identical to that of C57BL/6 controls, including expression of the type I lectin DEC205/CD205 and E-cadherin.…”

Section: Discussionsupporting

confidence: 91%

Disruption of thelangerin/CD207Gene Abolishes Birbeck Granules without a Marked Loss of Langerhans Cell Function

Kissenpfennig

Aït-Yahia²,

Clair-Moninot³

et al. 2005

Molecular and Cellular Biology

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103

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Langerin is a C-type lectin expressed by a subset of dendritic leukocytes, the Langerhans cells (LC).Langerin is a cell surface receptor that induces the formation of an LC-specific organelle, the Birbeck granule (BG). We generated a langerin ؊/؊ mouse on a C57BL/6 background which did not display any macroscopic aberrant development. In the absence of langerin, LC were detected in normal numbers in the epidermis but the cells lacked BG. LC of langerin ؊/؊ mice did not present other phenotypic alterations compared to wild-type littermates. Functionally, the langerin ؊/؊ LC were able to capture antigen, to migrate towards skin draining lymph nodes, and to undergo phenotypic maturation. In addition, langerin ؊/؊ mice were not impaired in their capacity to process native OVA protein for I- Dendritic cells (DC) are the most potent leukocytes to mediate the rapid initiation of a primary immune response (7). DC are bone marrow-derived leukocytes, localized in most tissues including primary and secondary lymphoid organs. In the periphery, most DC are in an immature state and are able to capture antigenic molecules via unique endocytic receptors or by fluid-phase macropinocytosis. This process generally leads to a first step of DC maturation, concomitant with their migration to secondary lymphoid organs. DC can subsequently activate naive CD4 ϩ T or CD8 ϩ T lymphocytes if peptides processed from native antigenic molecules are displayed on cell surface major histocompatibility complex (MHC) class II or I molecules in conjunction with cosignaling molecules (40).DC heterogeneity is a common feature of mice and humans. Precursor cell populations, anatomical localization, morphology, phenotype, and functions determine the type of DC. However, the origin of different DC subsets is still controversial (4). A particular subset of DC is represented by Langerhans cells (LC), which are immature DC present in the epidermis and mucosal epithelium (53, 71). LC can be generated either from myeloid precursors (77, 78) or from CD4 low lymphoid precursors (2). LC express a number of cell surface receptors including CD205/DEC205, Fc␥ and Fcε receptors, and langerin/ CD207 (70,72).Langerin is a C-type lectin oriented in a type II configuration and featuring a single carbohydrate recognition domain in its extracellular region (72). Langerin molecules oligomerize as trimers at the cell surface and display Ca 2ϩ -dependent binding specificity for mannose, N-acetyl-glucosamine, and fucose (61, 72). Langerin is a potent inducer of Birbeck granules (BG), the hallmark organelles of LC, which consist of pentalamellar and zippered membranes at the electron microscopic level (12,75). In addition to inducing BG formation, langerin is an endocytic receptor involved in the trafficking of exogenous mannosylated ligands from the cell surface into intracellular BG compartments (71).To further explore the role of langerin and BG, we generated C57BL/6 mice with a targeted disruption of the langerin gene. Although MHC class II-positive LC were detected in n...

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Section: Discussionsupporting

confidence: 91%

Disruption of thelangerin/CD207Gene Abolishes Birbeck Granules without a Marked Loss of Langerhans Cell Function

Kissenpfennig

Aït-Yahia²,

Clair-Moninot³

et al. 2005

Molecular and Cellular Biology

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103

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“…On day 0, Langerin expression was undetectable in MHCII + LCs in the epidermis, as has been previously reported (34) (Fig. 3A).…”

Section: Tbr1-deficient Lcs Spontaneously Mature In Situ and Gain Emisupporting

confidence: 87%

“…3) (i.e., once the confluent LC network had been established) (34). In TbR1-deficient LCs, this upregulation of Langerin was diminished.…”

Section: Discussionmentioning

confidence: 99%

TGF-β Is Required To Maintain the Pool of Immature Langerhans Cells in the Epidermis

Kel

Girard-Madoux

Reizis

et al. 2010

The Journal of Immunology

151

142

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“…Langerin expression and, as a consequence, p14 depletion sets in markedly later when the cells have already reached the epidermis. 38 Finally, we investigated the molecular mechanism underlying the loss of LCs by means of BMDC cultures derived from CD11c-p14 del and control mice. The adaptor molecule p14 has been shown to contribute to ERK as well as mTORC1 signaling in nonimmune 6,9,10,12 and also immune cells.…”

Section: Discussionmentioning

confidence: 99%

The late endosomal adaptor molecule p14 (LAMTOR2) represents a novel regulator of Langerhans cell homeostasis

Sparber

Scheffler

Amberg

et al. 2014

Blood

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Ontogeny of Langerin/CD207 Expression in the Epidermis of Mice

Cited by 60 publications

References 10 publications

Disruption of thelangerin/CD207Gene Abolishes Birbeck Granules without a Marked Loss of Langerhans Cell Function

Disruption of thelangerin/CD207Gene Abolishes Birbeck Granules without a Marked Loss of Langerhans Cell Function

TGF-β Is Required To Maintain the Pool of Immature Langerhans Cells in the Epidermis

The late endosomal adaptor molecule p14 (LAMTOR2) represents a novel regulator of Langerhans cell homeostasis

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