Ontogeny of the digestive enzyme activities in hatchery produced Beluga (Huso huso)

Asgari, Reza; Rafiee, Gholamreza; Eagderi, Soheil; Noori, Farzaneh; Agh, Naser; Poorbagher, Hadi; Gisbert, Enric

doi:10.1016/j.aquaculture.2013.08.014

Cited by 23 publications

(27 citation statements)

References 47 publications

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“…In the present study, all of the analyzed digestive enzymes were present in American shad larvae at hatching, with the exception of pepsin, which indicated that the larvae have the initial ability to digest nutrients from live food, even before the onset of exogenous feeding. This finding is in agreement with the early detection of enzymes in a variety of other fish species (Rønnestad and Morais 2007;Asgari et al 2013;Farhoudi et al 2013;Nazemroaya et al 2015). In addition, the results also illustrate that the early appearance of enzymes was triggered by underlying genetic mechanisms, rather than induced by dietary intake (Buddington and Diamond 1989;Lazo et al 2000).…”

Section: Discussionsupporting

confidence: 89%

“…This observation also indicated that the alkaline proteases are not the permanent major enzymes for proteases digestion, but suggested the temporal changes and stabilization of different proteases, i.e., from trypsin to pepsin (Lazo et al 2007). Similar patterns in trypsin levels have been observed in H. huso (Asgari et al 2013), A. regius (Suzer et al 2013), herring (Clupea harengus) (Pedersen and Andersen. 1992), and white bream (Diplodus sargus) (Cara et al 2003).…”

Section: Discussionmentioning

confidence: 52%

“…The synthesis of trypsin during embryogenesis and hatching plays a vital role in the cleavage of proteins in the yolk, as well as the digestion and breakage of the egg chorion (Dettlaff et al 1993;Sveinsdóttir et al 2006;Gisbert et al 2009). The early synthesis of trypsin is thought to be genetically pre-programmed, and it has been reported in other species (Zouiten et al 2008;Asgari et al 2013;Martínez-Lagos et al 2014). Thus, the presence of trypsin observed in the present study confirms its important role in physiological functions during the early development of American shad larvae.…”

Section: Discussionmentioning

confidence: 94%

“…This result also revealed a regulative digestive ability of the larvae to maintain a continuous production of certain enzymes according to different types of live food. The subsequent decline that was observed until 30 DAH might have been associated with larvae growth, feeding regime alteration, or brush border membrane maturation (Cara et al 2003;Suzer et al 2006;Zouiten et al 2008;Asgari et al 2013). From then on, specific trypsin activity rapidly decreased, which seemed to coincide with the establishment of nearly fully functional gastric cells and increased pepsin activity, and the progressive shift from an alkaline to an acidic digestion mode (Pradhan et al 2013).…”

Section: Discussionmentioning

confidence: 99%

“…This valuable information can then be used to synchronize feeding programs with larval development and to develop age-specific nutritional programs, thereby improving larval mass rearing (Zambonino-Infante et al 2009). Detailed information regarding the ontogenetic development of digestive enzymes and nutritional requirements has been reported for several different fish larvae, including spotted rose snapper (Lutjanus guttatus) (MoguelHernández et al 2014), common dentex (Dentex dentex) ), beluga (Huso huso) (Asgari et al 2013), yellow tail (Seriola lalandi) (Chen et al 2006), grouper (Epinephelus coioides) (Feng et al 2008), and meagre (Argyrosomus regius) (Suzer et al 2013). Kolkovski (2001) suggested that fish larvae may have enough levels of digestive enzymes to digest live food at first feeding, but may be not enough to digest formulated diet.…”

Section: Introductionmentioning

confidence: 99%

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Developmental changes in digestive enzyme activity in American shad, Alosa sapidissima, during early ontogeny

Gao

Liu

Guan

et al. 2016

Fish Physiol Biochem

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In order to assess the digestive physiological capacity of the American shad Alosa sapidissima and to establish feeding protocols that match larval nutritional requirements, we investigated the ontogenesis of digestive enzymes (trypsin, amylase, lipase, pepsin, alkaline phosphatase, and leucine aminopeptidase) in larvae, from hatching to 45 days after hatching (DAH). We found that all of the target enzymes were present at hatching, except pepsin, which indicated an initial ability to digest nutrients and precocious digestive system development. Trypsin rapidly increased to a maximum at 14 DAH. Amylase sharply increased until 10 DAH and exhibited a second increase at 33 DAH, which coincided with the introduction of microdiet at 30 DAH, thereby suggesting that the increase was associated with the microdiet carbohydrate content. Lipase increased until 14 DAH, decreased until 27 DAH, and then increased until 45 DAH. Pepsin was first detected at 27 DAH and then sharply increased until 45 DAH, which suggested the formation of a functional stomach. Both alkaline phosphatase and leucine aminopeptidase markedly increased until 18 DAH, which indicated intestinal maturation. According to our results, we conclude that American shad larvae possess the functional digestive system before mouth opening, and the significant increases in lipase, amylase, pepsin, and intestinal enzyme activities between 27 and 33 DAH suggest that larvae can be successfully weaned onto microdiets around this age.

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