Oocyte maturation, embryo development and gene expression following two different methods of bovine cumulus-oocyte complexes vitrification

Azari, Mehdi; Kafi, M.; Ebrahimi, Bita; Fatehi, Roya; Jamalzadeh, Mahboobeh

doi:10.1007/s11259-016-9671-8

Cited by 29 publications

(19 citation statements)

References 43 publications

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“…We have not detected differences in the amount and the integrity of the total RNA in intact and vitrified/thawed groups of human ovarian tissue samples (data not shown). However, it has been previously shown that there is a statistically significant difference in transcript abundance in cells before and after vitrification [23][24][25]. Therefore, distortion of results can occur in the following cases: normalization to the total RNA or normalization to the mRNA of nonrepresentative reference gene.…”

Section: Discussionmentioning

confidence: 99%

Selection of stable expressed reference genes in native and vitrified/thawed human ovarian tissue for analysis by qRT-PCR and Western blot

Никишин

Filatov

Kiseleva

et al. 2018

J Assist Reprod Genet

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Selection of suitable reference genes is the first aim of any research dedicated to the investigation of gene expression, because the interpretation of obtained results largely depends on selection of appropriate reference genes. Nowadays, there are many mathematical approaches allowing to select not only single reference gene but also a group of the most stably expressed reference genes. The use of mathematical models which take into account multiple reference genes will allow to obtain more accurate data on the expression of target genes.

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Section: Discussionmentioning

confidence: 99%

Selection of stable expressed reference genes in native and vitrified/thawed human ovarian tissue for analysis by qRT-PCR and Western blot

Никишин

Filatov

Kiseleva

et al. 2018

J Assist Reprod Genet

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“…Oocytes were loaded onto the tip of Cryotop (Kitazato Supply Co., Tokyo, Japan) in a small volume of vitrification solution and plugged into liquid nitrogen immediately. Warming of vitrified oocytes was done by immersing the Cryotop tip directly in a 37ºC warming solution composed of HM and 1 M sucrose for 1 min, followed by treatment with HM supplemented with 0.5 M for 3 min (15). Afterward, the retrieved oocytes were washed and transferred to HM until the next procedure.…”

Section: Experiments 2: Effects Of Niacin Addition In Ivm Medium On Crmentioning

confidence: 99%

Niacin improves maturation and cryo-tolerance of bovine in vitro matured oocytes: An experimental study

Kafi

Ashrafi

Azari

et al. 2019

IJRM

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Background: Nicotinic acid (niacin) is a broad-spectrum lipid-modifying agent that has potent antioxidant properties and reduces the production of lipid peroxidation. Objective: The purpose of the present study was to investigate the maturation, embryo development and cryo-tolerance merit, and levels of malondialdehyde (MDA), total oxidant status, and total antioxidant capacity following the supplementation of bovine oocytes maturation medium with different concentrations of niacin. Materials and Methods: Immature cumulus-oocyte complexes were cultured in tissue culture medium-199 maturation media supplemented with 0, 100, 200, and 400 µM niacin under a standard in vitro culture condition. After 24 hr of culture, the nuclear maturation rate was assessed. Then, two groups of immature cumulus-oocyte complexes were cultured in TCM-199 either with or without 400 µM niacin and evaluated for embryo development. Also, matured cumulus-oocyte complexes in both groups were frozen using a standard vitrification procedure. After vitrification, oocytes were warmed in two steps and evaluated for embryo development. In addition, the level of total antioxidant capacity, total oxidant status, and MDA were measured. Results: The results indicated that although the treatment with 400µM niacin increased in vitro nuclear maturation (87.6 ± 5.3), it did not improved the embryo development to the blastocyst stage. Higher cleavage and blastocyst rates were observed in vitrified oocytes that were cultured with supplemented 400 µM niacin compared to the control group (without niacin) (53.6 ± 2.7 and 10.6 ± 1.6 vs. 46.2 ± 4.1 and 6.3 ± 2.4, respectively). Also, the addition of 400 µM niacin to the maturation media could decrease MDA levels after maturation. Conclusion: Niacin could improve the quality of in vitro embryo production (IVP) embryos and tolerance of bovine oocytes to vitrification. Key words: Bovine, Embryonic development, Niacin, Oocytes, Vitrification.

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“…Frozen semen which previously tested in the laboratory for IVF was used for fertilization. Motile spermatozoa from frozen/thawed semen were obtained using the swim-up method and were then added to wells containing oocytes at a final concentration of 10 6 spermatozoa ml−1 (12). Oocytes and spermatozoa were co-incubated at 38.5 • C for 18 h in 5% CO 2 .…”

Section: In Vitro Fertilizationmentioning

confidence: 99%

“…Communication between the oocyte and its surrounding cumulus cells is an important event for development of a competent oocyte (12). Growth differentiation factor (GDF9) and steroidogenic acute regulatory (StAR) proteins play a major role in oocyte developmental competence (13,14).…”

Section: Introductionmentioning

confidence: 99%

Effects of Pre-ovulatory Follicular Fluid of Repeat Breeder Dairy Cows on Bovine Fertility Transcriptomic Markers and Oocytes Maturation and Fertilization Capacity

et al. 2021

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The current study aimed to determine the effects of the preovulatory follicular fluid (FF) of normal heifer (NH) and repeat breeder cows with subclinical endometritis (SCE) or without (nSCE) on oocyte maturation (Experiment 1) and fertilization rates (Experiment 2). Moreover, the pattern of gene expression of cumulus oocyte-complexes was evaluated in Experiment 1. In Experiment 1, nuclear maturation in the nSCE group was higher, compared to that in the SCE group (P = 0.05). In addition, the oocyte nuclear maturation in the normal heifer was significantly higher, in comparison to that of SCE groups (P < 0.05). Furthermore, the mean percentage of normal oocyte fertilization was higher in the nSCE group, compared to that in the SCE group (P < 0.05). The expressions of growth differentiation factor, GDF9; steroidogenic acute regulatory, StAR and follicle-stimulating hormone receptor, FSHr in the NH group were significantly higher, compared to those in SCE and nSCE groups (P < 0.05). Moreover, the expressions of all genes in the nSCE group were not significant, in comparison to those in the SCE group (P > 0.05). The supplementation of oocyte maturation medium with FF from pre-ovulatory follicles of repeat breeder cows resulted in less oocyte maturation and cumulus cell expansion. In conclusion, the lower fertility in RB cows could be ascribed to the lower oocyte maturation rate and less expression of GDF9, StAR, and FSHr in the cumulus-oocyte complexes.

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Oocyte maturation, embryo development and gene expression following two different methods of bovine cumulus-oocyte complexes vitrification

Abstract: Expression of oocyte maturation genes was affected by vitrification procedure and conditions. Using EG alone for vitrification of bovine immature COCs, resulted in higher expression of GDF9, BMP15 and production of more in vitro matured and cleaved oocytes.

Cited by 29 publications

References 43 publications

Selection of stable expressed reference genes in native and vitrified/thawed human ovarian tissue for analysis by qRT-PCR and Western blot

Selection of stable expressed reference genes in native and vitrified/thawed human ovarian tissue for analysis by qRT-PCR and Western blot

Niacin improves maturation and cryo-tolerance of bovine in vitro matured oocytes: An experimental study

Effects of Pre-ovulatory Follicular Fluid of Repeat Breeder Dairy Cows on Bovine Fertility Transcriptomic Markers and Oocytes Maturation and Fertilization Capacity

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