Oocyte-secreted factros regulate granulosa cell steroidogenesis

Vanderhyden, Barbara C.

doi:10.1017/s0967199400003324

Cited by 16 publications

(4 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because oocyte removal from a preovulatory follicle results in spontaneous luteinization of granulosa cells (51), the oocyte is the likely source of this factor. In addition, oocyte-secreted factors such as GDF9 prevent mural granulosa cell luteinization (52)(53)(54)(55)(56)(57). GDF9 also suppresses Lhcgr (56,57).…”

Section: Discussionmentioning

confidence: 99%

Premature Luteinization and Cumulus Cell Defects in Ovarian-SpecificSmad4Knockout Mice

Pangas¹,

Li²,

Robertson

et al. 2006

Molecular Endocrinology

171

161

View full text Add to dashboard Cite

SMAD4 is a central component of the TGFbeta superfamily signaling pathway. Within the ovary, TGFbeta-related proteins play crucial roles in controlling granulosa cell growth, differentiation, and steroidogenesis. To study the in vivo roles of SMAD4 during follicle development, we generated an ovarian conditional knockout of Smad4 using the cre/loxP recombination system. Smad4 ovarian-specific knockout mice are subfertile with decreasing fertility over time and multiple defects in folliculogenesis. Regulation of steroidogenesis is disrupted in the Smad4 conditional knockout, leading to increased levels of serum progesterone. In addition, severe cumulus cell defects are present both in vivo and when assayed in vitro. These findings demonstrate that disrupting signaling through SMAD4 in the ovarian granulosa cells leads to premature luteinization of granulosa cells and eventually premature ovarian failure, thereby demonstrating key in vivo roles of TGFbeta superfamily signaling in the timing of granulosa cell differentiation.

show abstract

Section: Discussionmentioning

confidence: 99%

Premature Luteinization and Cumulus Cell Defects in Ovarian-SpecificSmad4Knockout Mice

Pangas¹,

Li²,

Robertson

et al. 2006

Molecular Endocrinology

171

161

View full text Add to dashboard Cite

show abstract

“…Current studies on mouse oocytes have shown that the oocytes secrete a factor(s) affecting the functions of the granulosa cells. A conditioned medium of mouse oocytes stimulates the proliferation of granulosa cells (Vanderhyden et al, 1992), steroidogenesis (Vanderhyden & Tonary, 1995;Vanderhyden, 1996), synthesis of hyaluronic acid and the expansion of cumulus cells (Buccione et al, 1990;Vanderhyden et al, 1990Vanderhyden et al, , 1992Eppig et al, 1993) in response to FSH. This suggests that such effects of the mouse oocytes on the granulosa cells are mediated in a paracrine fashion (Eppig, 1994).…”

Section: Discussionmentioning

confidence: 99%

Promotion of follicular antrum formation by pig oocytes in vitro

Shen

Miyano

Kato

1998

Zygote

View full text Add to dashboard Cite

SummaryPig oocyte–cumulus–granulosa cell complexes (OCG complexes) from pig early antral follicles reorganise an antrum under the stimulation of FSH. The purpose of this study was to examine the role of the oocytes in antrum formation. In the first experiment, oocyte–cumulus complexes were removed from pig OCG complexes, and the antrum formation of parietal granulosa cells themselves (PGs) was examined. Antrum formation by sham-operated OCG complexes (OC/G complexes), in which the connections between the oocyte–cumulus complexes and the parietal granulosa cells had been disrupted, was also examined. The complexes were cultured for 8 days in collagen gels in the presence of 10ng/ml FSH. Antra were formed in about 60% of the intact OCG complexes and the sham-operated OCG complexes, while only 20% of the PGs formed antra. In the second experiment, oocyte–cumulus complexes in the OCG complexes were replaced by denuded oocytes (O/G complexes) or Sephadex G-25 beads (B/G complexes) similar in diameter to the oocytes, and the two types of complexes were cultured under the same conditions. The O/G complexes formed antra to a similar extent as the OC/G complexes, whereas the B/G complexes scarcely formed any antra. The histological sections showed that the granulosa cells in the OC/G and O/G complexes were in intimate contact with each other and retained a shape similar to those in the ovarian follicles, while the granulosa cells in the PGs and B/G complexes became quite irregular in shape. These results suggest that pig oocytes promote contact between the granulosa cells to induce antrum formation in a physiological manner.

show abstract

“…The oocytes, on the other hand, also have a crucial role in the regulation of cumulus and granulosa cells. From the production of paracrine factors, the oocyte is responsible for the modulation and proliferation of the function of granulosa cells, with a crucial role in the steroidogenesis process thereof ( Vanderhyden and Tonary, 1995 ; Vanderhyden, 1996 ; Eppig et al, 1997 , 1998 ; Gilchrist et al, 2006 ; Su et al, 2009 ; Young and McNeilly, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Role of Growth Differentiation Factor 9 and Bone Morphogenetic Protein 15 in Ovarian Function and Their Importance in Mammalian Female Fertility — A Review

Castro

Cruz

Leal

2015

Asian Australas. J. Anim. Sci

View full text Add to dashboard Cite

Growth factors play an important role during early ovarian development and folliculogenesis, since they regulate the migration of germ cells to the gonadal ridge. They also act on follicle recruitment, proliferation/atresia of granulosa cells and theca, steroidogenesis, oocyte maturation, ovulation and luteinization. Among the growth factors, the growth differentiation factor 9 (GDF9) and the bone morphogenetic protein 15 (BMP15), belong to the transforming growth factor beta (TGF-β) superfamily, have been implicated as essential for follicular development. The GDF9 and BMP15 participate in the evolution of the primordial follicle to primary follicle and play an important role in the later stages of follicular development and maturation, increasing the steroidogenic acute regulatory protein expression, plasminogen activator and luteinizing hormone receptor (LHR). These factors are also involved in the interconnections between the oocyte and surrounding cumulus cells, where they regulate absorption of amino acids, glycolysis and biosynthesis of cholesterol cumulus cells. Even though the mode of action has not been fully established, in vitro observations indicate that the factors GDF9 and BMP15 stimulate the growth of ovarian follicles and proliferation of cumulus cells through the induction of mitosis in cells and granulosa and theca expression of genes linked to follicular maturation. Thus, seeking greater understanding of the action of these growth factors on the development of oocytes, the role of GDF9 and BMP15 in ovarian function is summarized in this brief review.

show abstract

Oocyte-secreted factros regulate granulosa cell steroidogenesis

Cited by 16 publications

References 19 publications

Premature Luteinization and Cumulus Cell Defects in Ovarian-SpecificSmad4Knockout Mice

Premature Luteinization and Cumulus Cell Defects in Ovarian-SpecificSmad4Knockout Mice

Promotion of follicular antrum formation by pig oocytes in vitro

Role of Growth Differentiation Factor 9 and Bone Morphogenetic Protein 15 in Ovarian Function and Their Importance in Mammalian Female Fertility — A Review

Contact Info

Product

Resources

About