Open Design 3D-Printable Adjustable Micropipette that meets ISO Standard for Accuracy

Brennan, Martin D.; Bokhari, Fahad F.; Eddington, David T.

doi:10.1101/109231

Cited by 7 publications

(6 citation statements)

References 29 publications

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“…This open‐source idea opens the door to an inexpensive and fast development of individual labware. Considering the short innovation cycles of new 3D‐printing techniques it might be just a matter of time until 3D‐printed labware can meet the same standards as conventionally manufactured labware .…”

Section: Discussionmentioning

confidence: 99%

Additive manufactured customizable labware for biotechnological purposes

Raddatz

Vries

Austerjost

et al. 2017

Engineering in Life Sciences

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Yet already developed in the 1980s, the rise of 3D printing technology did not start until the beginning of this millennium as important patents expired, which opened the technology to a whole new group of potential users. One of the first who used this manufacturing tool in biotechnology was Lücking et al. in 2012, demonstrating potential uses . This study shows applications of custom‐built 3D‐printed parts for biotechnological experiments. It gives an overview about the objects' computer‐aided design (CAD) followed by its manufacturing process and basic studies on the used printing material in terms of biocompatibility and manageability. Using the stereolithographic (SLA) 3D‐printing technology, a customizable shake flask lid was developed, which was successfully used to perform a bacterial fed‐batch shake flask cultivation. The lid provides Luer connectors and tube adapters, allowing both sampling and feeding without interrupting the process. In addition, the digital blueprint the lid is based on, is designed for a modular use and can be modified to fit specific needs. All connectors can be changed and substituted in this CAD software‐based file. Hence, the lid can be used for other applications, as well. The used printing material was tested for biocompatibility and showed no toxic effects neither on mammalian, nor on bacteria cells. Furthermore an SDS‐PAGE‐comb was drawn and printed and its usability evaluated to demonstrate the usefulness of 3D printing for everyday labware. The used manufacturing technique for the comb (multi jet printing, MJP) generates highly smooth surfaces, allowing this application.

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Section: Discussionmentioning

confidence: 99%

Additive manufactured customizable labware for biotechnological purposes

Raddatz

Vries

Austerjost

et al. 2017

Engineering in Life Sciences

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“…Although we provide examples for plating and replication of bacterial samples to determine MIC on agar, plus dip-slides and plates for multichannel agar media, many different labware components can be readily designed and tested. Other equipment in the microbiology laboratory has also been designed and 3D printed including digital microscopy (Sharkey et al 2016), centrifuge (Byagathvalli et al 2019) and micropipettes (Brennan et al 2018). The open publication of these designs will support a new wave of microbiology innovation across the globe.…”

Section: Customised Pin Replicator Allows Personalised Configuration For Plating Onto Antibiotic Plates To Measure Minimum Inhibitory Conmentioning

confidence: 99%

Methods for rapid prototyping novel labware: using CAD and desktop 3D printing in the microbiology laboratory

Diep

Ray

Edwards

2021

Letters Applied Microbiology

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Significance and Impact of the Study: We present methods for designing and 3D printing microbiological labware offering alternatives to off-the-shelf consumables that allow low-cost rapid prototyping and customisation of microbial culture tools. We demonstrate customised 3D printed rapid prototype solid medium culture dishes and dip-slides, and modified inoculating loops and customisable replicating pins for plating bacteria. 3D printed labware can offer local production to avoid dependence on commercial suppliers, rapid customisation of existing designs, and rapid evaluation of entirely new tools. Using 3D printing to develop novel labware tailored to simplify routine work in the microbiology laboratory can save time and labour compared to relying on off-the-shelf mass-manufactured labware.

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“…Beyond medical applications we also want to develop tools relevant for scienti c experiments. Already, micropipettes and micro-centrifuge in line with protocols of Brennan et al (35) and the Thingiverse community (36, 37) have been printed and optimised in the lab. This was practically possible in the parlance of "the €100 lab", where 3D-printable open-source platform is deployable for state-of-the-art techniques like optogenetics, uorescence microscopy, and temperature control as usually necessary in Drosophila, zebra sh and Caenorhabditis elegans behavioural assays (38,39).…”

Section: Beyond the Covid Daysmentioning

confidence: 99%

PPEs From Nigerian Academia: Flattening The COVID-19 Curve With 3D Printing And Locally Sourced Intervention

Folarin

Chagas

Bukar

et al. 2021

Preprint

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Background:Personal protective equipment (PPE) supply shortages were a big issue in the fight against COVID-19 around the world at the onset of the pandemic, requiring all communities to find innovative ways to make and supply PPE for health workers, vulnerable people and the general public. To this end and in line with the WHO’s call for 40% raise in PPE production across the world, we sought to alleviate the PPE shortage and to support our local communities using 3D printing (additive manufacturing) and free and open-source hardware (FOSH). Methods:Utilising a 3D printer and off-the-shelf components, reusable face shields and face masks were produced based on open source designs. Off-the-shelf components included transparent polyvinyl chloride (PVC) visor, ribbon tapings and elastic bands for the face shields, as well as cushioning pads (clothed foams) and filters (sensitive tissues) for the face masks. Hygienic measures employed during fabrication included the assembly following safety protocols, disinfection of products with ultraviolet bactericidal lamps, ensuring hygiene during collection and distribution. Users’ real-life experience and feedback were utilised to modify and improve on quality and adaptability of the designs.Results:In a period of three months, over 400 reusable 3D-printed face shields and face masks were produced by a team of academics, for health practitioners, other professionals and people across the Olabisi Onabanjo University community and other cities within Ogun and Lagos states. Conclusions:More awareness is generally required on the potentials of 3D printing and FOSH in the global south, particularly in universities and research institutions where innovative alternatives to expensive equipment remain vital. Our feat corroborated and advocated these potentials in a low-income setting like Nigeria, where the immediate response and synergy between academics, and researchers yielded a substantial number of PPE to front line workers, in a timely manner at the peak of the viral transmission and lockdowns- a period wherein manufacturers of PPEs struggled to establish their commercial logistics. We emphasize the need for university managements to support academics and researchers strongly to deliver on much needed community support in crises time, and encourage governmental and non-governmental bodies to consider investing in this innovative self-reliant perspective through their research funding and managemnt programmes in a bid to achieving a lot more with the less funds.Trial registration:Not applicable.

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Open Design 3D-Printable Adjustable Micropipette that meets ISO Standard for Accuracy

Cited by 7 publications

References 29 publications

Additive manufactured customizable labware for biotechnological purposes

Additive manufactured customizable labware for biotechnological purposes

Methods for rapid prototyping novel labware: using CAD and desktop 3D printing in the microbiology laboratory

PPEs From Nigerian Academia: Flattening The COVID-19 Curve With 3D Printing And Locally Sourced Intervention

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