Open tubular supercritical fluid chromatography of triterpene acids from Dysoxylum pettigrewianum (Meliaceae)

Abstract

SummaryOpen tubular supercritical fluid chromatography (SFC) with carbon dioxide as the mobile phase has been investigated for the separation of four triterpene acids, two of which were structural isomers. These compounds, isolated from Dysoxylum pettigrewiaaum (Meliaceae), were chromatographed on three stationary phases with different selectivities. 30% -biphenylmethylpolysiloxane and polyethylene glycol (Carbowax 20M) phases exhibited poor chromatographic selectivity for the compounds. The separation was improved by employing the shape selectivity of a liquid crystalline biphenylcarboxylate ester polysiloxane and utilizing simultaneous temperature and pressure programming. The elution order was explained on the basis of the molecular structures of the triterpene acids, and their interaction with the liquid crystal phase.pounds with similar chemical structures. High chromatographic efficiency and stationary phase selectivity are therefore mandatory for successful analysis.

Supercritical Fluid Chromatography and Extraction

“…The separation was performed on a "bare" silica packing with methanol-modified CO 2 . Sewram et al used the molecular shape selectivity of a liquid crystal-modified polysiloxane, open-tubular SFC column to separate triterpene acids, including geometric isomers, from Dysoxylum pettigrewianum (188). The separations were not successful on a biphenylmodified polysiloxane or a poly(ethylene glycol)-coated column.…”

Columns in analytical‐scale supercritical fluid chromatography: From traditional to unconventional chemistries

“…The first report on liquid crystals used as stationary phase in SFC dates to 1985, when new liquid crystalline polymers were synthesized to overcome challenges connected with their use in capillary columns [256]. Sewram et al compared the selectivity of biphenylmethylpolysiloxane, polyethylene glycol, and a liquid crystalline biphenylcarboxylate ester polysiloxane in a separation of triterpene acids isolated from plants with the liquid crystal stationary phase showing the best resolution of target analytes [257]. Polyacrylate liquid crystalline compounds were coated on silica and glass capillaries and tested as stationary phases for the separation of isomeric PAH [256].…”

Evaluation of the antifungal properties of chitosan coating on cut apples using a non‐invasive image analysis technique

“…The toxins are recovered with chloroform and the intensity detected using liquid chromatography (HPLC). 32,33 Such a procedure, in spite of being accurate, makes it impossible to track fungal growth in the same sample.…”