The analysis of energy consumption in a hybrid drive system of a passenger car in real road conditions is an important factor determining its operational indicators. The article presents energy consumption analysis of a car equipped with an advanced Plug-in Hybrid Drive System (PHEV), driving in real road conditions on a test section of about 51 km covered in various environmental conditions and seasons. Particular attention was paid to the energy consumption resulting from the cooperation of two independent drive units, analyzed in terms of the total energy expenditure. The energy consumption obtained from fuel and energy collected from the car’s batteries for each run over the total distance of 12,500 km was summarized. The instantaneous values of energy consumption for the hybrid drive per kilometer of distance traveled in car’s real operating conditions range from 0.6 to 1.4 MJ/km, with lower values relating to the vehicle operation only with electric drive. The upper range applies to the internal combustion engine, which increases not only the energy expenditure in the TTW (Tank-to-Wheel) system, but also CO2 emissions to the environment. Based on the experimental data, the curves of total energy consumption per kilometer of the road section traveled were determined, showing a close correlation with the actual operating conditions. Obtained values were compared with homologation data from the WLTP test of the tested passenger car, where the average value of energy demand is 1.1 MJ/km and the CO2 emission is 23 g/km.