Operation strategies of n-DAMO and Anammox process based on microbial interactions for high rate nitrogen removal from landfill leachate

Nie, Wen-Bo; Xie, Guo-Jun; Ding, Jie; Peng, Lai; Lü, Yang; Tan, Xin; Hao, Yue; Liu, Bingfeng; Xing, Defeng; Meng, Jia; Han, Hongjun; Ren, Nanqi

doi:10.1016/j.envint.2020.105596

Cited by 47 publications

(22 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…28 Hybridization was performed using 40% formamide as described in previous studies. 29,30 Oligonucleotide probes (Table S2) were purchased with specific fluorescence labels from Sangon Biotech Co., Ltd. (Shanghai, China), which were evaluated as described by Hugenholtz et al 31 Hybridized slides were examined via a Zeiss (LSM800) confocal laser scanning microscope (Carl Zeiss Meditec AG, Jena, Germany).…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…Biomass samples were collected from the reactors at different times during operation, subsequently fixed by a PBS-buffered (pH = 7.2) 4% (v/v) paraformaldehyde (Sigma, Germany) solution for 45 min at room temperature . Hybridization was performed using 40% formamide as described in previous studies. , Oligonucleotide probes (Table S2) were purchased with specific fluorescence labels from Sangon Biotech Co., Ltd. (Shanghai, China), which were evaluated as described by Hugenholtz et al Hybridized slides were examined via a Zeiss (LSM800) confocal laser scanning microscope (Carl Zeiss Meditec AG, Jena, Germany).…”

Section: Materials and Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Anaerobic Oxidation of Methane Coupled with Dissimilatory Nitrate Reduction to Ammonium Fuels Anaerobic Ammonium Oxidation

Nie

Ding

Xie

et al. 2020

Environ. Sci. Technol.

Self Cite

View full text Add to dashboard Cite

Nitrate/nitrite-dependent anaerobic methane oxidation (n-DAMO) is critical for mitigating methane emission and returning reactive nitrogen to the atmosphere. The genomes of n-DAMO archaea show that they have the potential to couple anaerobic oxidation of methane to dissimilatory nitrate reduction to ammonium (DNRA). However, physiological details of DNRA for n-DAMO archaea were not reported yet. This work demonstrated n-DAMO archaea coupling the anaerobic oxidation of methane to DNRA, which fueled Anammox in a methane-fed membrane biofilm reactor with nitrate as only electron acceptor. Microelectrode analysis revealed that ammonium accumulated where nitrite built up in the biofilm. Ammonium production and significant upregulation of gene expression for DNRA were detected in suspended n-DAMO culture with nitrite exposure, indicating that nitrite triggered DNRA by n-DAMO archaea. 15N-labeling batch experiments revealed that n-DAMO archaea produced ammonium from nitrate rather than from external nitrite. Localized gradients of nitrite produced by n-DAMO archaea in biofilms induced ammonium production via the DNRA process, which promoted nitrite consumption by Anammox bacteria and in turn helped n-DAMO archaea resist stress from nitrite. As biofilms predominate in various ecosystems, anaerobic oxidation of methane coupled with DNRA could be an important link between the global carbon and nitrogen cycles that should be investigated in future research.

show abstract

Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: Materials and Methodsmentioning

confidence: 99%

Anaerobic Oxidation of Methane Coupled with Dissimilatory Nitrate Reduction to Ammonium Fuels Anaerobic Ammonium Oxidation

Nie

Ding

Xie

et al. 2020

Environ. Sci. Technol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…In this study, 99.2% of influent TN was removed, and only 15.6 mg/L TN remained in the effluent, significantly reducing the organic matter demand. Due to the existence of NOB, the effluent of a typical partial nitrification–Anammox process had a relatively high level of nitrate (17–53 mg/L). − In PNA-SBR, NOB were efficiently inhibited by the step-feed with the intermittent aeration strategy: only small percentages of Nitrobacter (0.03%), Nitrospirae (0.48%), and Nitrotoga (0.12%) remained.…”

Section: Resultsmentioning

confidence: 99%

An Innovative Process for Mature Landfill Leachate and Waste Activated Sludge Simultaneous Treatment Based on Partial Nitrification, In Situ Fermentation, and Anammox (PNFA)

Zhang

Peng

Zhong

et al. 2021

Environ. Sci. Technol.

View full text Add to dashboard Cite

An innovative partial nitrification, in situ fermentation, and Anammox (PNFA) system was developed to achieve mature landfill leachate and waste activated sludge simultaneous treatment. Three separate sequencing batch reactors (SBRs) were used for partial nitrification (PN-SBR), integrated fermentation–denitrification (IFD-SBR), and partial nitrification–Anammox (PNA-SBR). After 200 days of continuous operation, a satisfactory nitrogen removal efficiency (NRE) of 99.2 ± 0.1% was obtained, with an effluent total nitrogen (TN) of 15.2 ± 3.2 mg/L. In IFD-SBR, the volatile fatty acids generated from fermentation drove efficient denitrification, obtaining sludge and nitrogen reduction rates of 4.2 ± 0.7 and 0.61 ± 0.04 kg/m3·day, respectively. Furthermore, unwanted fermentation metabolites (134.1 mg/L NH4 +-N) were further treated by PNA-SBR using a combination of step-feed and intermittent aeration strategies. In PNA-SBR, Anammox significantly contributed to 82.1% nitrogen removal, and Anammox bacteria (Candidatus Brocadia, 2.3%) mutually benefited with partially denitrifying microorganisms (Thauera, 4.2%), with 66.3% of generated nitrate reduced to nitrite and then reutilized in situ by Anammox. Compared with the conventional nitrification–denitrification process, PNFA reduced oxygen energy consumption, external carbon source dosage, and CO2 emission by 21.3, 100, and 38.9%, respectively, and obtained 50.1% external WAS reduction efficiency.

show abstract

“…230 For systems with both nitrate-DAMO and nitrite-DAMO, similar AOM rates of 3 × 10 −6 to 11.5 μmol CH 4 cm −3 d −1 (ref. 28, 50, 53, 92, 122 and 231–234) were reported. Currently, the highest AOM rate observed in bioreactors with nitrogen removal as the objective was from coculturing of DAMO archaea and Anammox bacteria.…”

Section: Some Of the Possibly Highest Aom Ratesmentioning

confidence: 99%

Significance of anaerobic oxidation of methane (AOM) in mitigating methane emission from major natural and anthropogenic sources: a review of AOM rates in recent publications

Gao

Wang

Lee

et al. 2022

Environ. Sci.: Adv.

View full text Add to dashboard Cite

show abstract

Operation strategies of n-DAMO and Anammox process based on microbial interactions for high rate nitrogen removal from landfill leachate

Cited by 47 publications

References 53 publications

Anaerobic Oxidation of Methane Coupled with Dissimilatory Nitrate Reduction to Ammonium Fuels Anaerobic Ammonium Oxidation

Anaerobic Oxidation of Methane Coupled with Dissimilatory Nitrate Reduction to Ammonium Fuels Anaerobic Ammonium Oxidation

An Innovative Process for Mature Landfill Leachate and Waste Activated Sludge Simultaneous Treatment Based on Partial Nitrification, In Situ Fermentation, and Anammox (PNFA)

Significance of anaerobic oxidation of methane (AOM) in mitigating methane emission from major natural and anthropogenic sources: a review of AOM rates in recent publications

Contact Info

Product

Resources

About