Ophiostomatoid fungi and their roles in &lt;i&gt;Quercus robur&lt;/i&gt; die-back in Tellermann forest, Russia

Selochnik, N. N.; Pashenova, Nataliya; Sidorov, E.; Wingfield, Michael J.; Linnakoski, Riikka

doi:10.14214/sf.1328

Cited by 9 publications

(3 citation statements)

References 26 publications

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“…Amplification of the studied gene region and purification of the PCR products were performed using the same protocols described in our previous publication [ 31 ]. The PCR reaction mixture contained 0.15 μL of MyTaq ™ DNA Polymerase (5 U/μl) (Bioline, Massachusetts, USA), 2.5 μl of MyTaq ™ Reaction Buffer (5×) containing dNTPs, MgCl 2 and enhancers for the optimal performance, and 0.50 μl of each primer (Whitehead Scientific Ltd, Cape Town, South Africa).…”

Section: Methodsmentioning

confidence: 99%

Seasonal Succession of Fungi Associated with Ips typographus Beetles and Their Phoretic Mites in an Outbreak Region of Finland

Linnakoski

Mahilainen

Harrington³

et al. 2016

PLoS ONE

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The ophiostomatoid fungi (Microascales and Ophiostomatales, Ascomycota) are common associates of Ips typographus, and include tree pathogens and species responsible for blue-stain of timber. Fungal assemblages associated with I. typographus have varied considerably between studies but few investigations have attempted to explain this variation. For this reason, we assessed the overall cultivable fungal diversity associated with I. typographus in a storm-felled spruce forest in south-eastern Finland. Fungi were isolated from the individually collected beetles as well as their phoretic mites in spring, summer and autumn, including different life stages of the beetle (hibernation, dispersal flight and first generation). The internal transcribed spacer (ITS) gene region was used to identify the fungi. A total of 32 operational taxonomic units (OTUs) were found and these resided in four fungal phyla/subphyla (24 Ascomycota, 2 Basidiomycota, 5 Mucoromycotina, 1 Mortierellomycotina) in association with adult bark beetles. Ophiostomatoid species were the most commonly detected fungal associates. A generalized linear model analysis showed a clear association between fungal communities and season, indicating seasonal succession among I. typographus-associated fungi. The season of sampling appears to be an important factor that has resulted in inconsistencies between results in previous studies. Many of these fungi were also found on phoretic mites and their presence or absence could have influenced variation in patterns of association.

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Section: Methodsmentioning

confidence: 99%

Seasonal Succession of Fungi Associated with Ips typographus Beetles and Their Phoretic Mites in an Outbreak Region of Finland

Linnakoski

Mahilainen

Harrington³

et al. 2016

PLoS ONE

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“…aspericollis , yet it was infrequently isolated from adult beetles and even more rarely cultured from gallery phloem. Ophiostoma quercus is a sapwood-staining ophiostomatoid fungus that occurs worldwide in a variety of hardwood genera (e.g., Quercus , Fagus , Acer , Betula , Eucalyptus , Castanea ) [ 60 ] and is considered to be saprotrophic or mildly pathogenic [ 72 , 73 ]. Ophiostoma quercus has been found on Alnus incana in Latvia [ 74 ], but our study is the first to report O .…”

Section: Discussionmentioning

confidence: 99%

Filamentous fungal associates of the alder bark beetle, Alniphagus aspericollis, including an undescribed species of Neonectria

et al. 2023

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Bark beetles (Coleoptera: Curculionidae; Scolytinae) are tree-infesting insects that consume subcortical tissues and fungi. Species capable of killing their host trees are most commonly associated with conifers, as very few bark beetle species infest and kill hardwood hosts directly. The alder bark beetle, Alniphagus aspericollis, is a hardwood-killing bark beetle that colonizes and kills red alder, Alnus rubra. Conifer-killing bark beetles have well-known associations with symbiotic ophiostomatoid fungi that facilitate their life histories, but it is unknown whether A. aspericollis has any fungal associates. This study was conducted to identify any consistent filamentous fungal associates of A. aspericollis and characterize the consistency of observed beetle–fungus relationships. Beetles and gallery phloem samples were collected from seven sites throughout the Greater Vancouver region in British Columbia, Canada. Filamentous fungi were isolated from these samples and identified by DNA barcoding using the internal transcribed spacer (ITS) region and other barcode regions for resolution to the species-level for the most dominant isolates. The most common fungal associate was a previously undescribed Neonectria major-like fungus, Neonectria sp. nov., which was isolated from ~67% of adult beetles, ~59% of phloem samples, and ~94% of the beetle-infested trees. Ophiostoma quercus was isolated from ~28% of adult beetles, ~9% of phloem samples, and ~56% of infested trees and deemed a casual associate of A. aspericollis, while a putatively novel species of Ophiostoma was more infrequently isolated from A. aspericollis and its galleries. Cadophora spadicis, a new record for red alder, was rarely isolated and is probably coincidentally carried by A. aspericollis. Overall, A. aspericollis was only loosely associated with ophiostomatoid fungi, suggesting that these fungi have little ecological significance in the beetle–tree interaction, while Neonectria sp. nov. may be a symbiote of A. aspericollis that is vectored by the beetle.

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“…Both species inhabit similar ecological niches. O. grandicarpum is known to occur mainly on Q. robur in Poland, the Czech Republic, Germany and Russia (Kehr and Wulf 1993; Kowalski and Butin 1989; Kowalski 1991; Novotný and Šrůtka 2004; Selochnik et al 2015). The fungus was only rarely isolated from A. dispar and Scolytus intricatus on Q. robur in our previous study (Jankowiak et al 2019).…”

Section: Discussionmentioning

confidence: 99%

Four new Ophiostoma species associated with conifer- and hardwood-infesting bark and ambrosia beetles from the Czech Republic and Poland

Jankowiak

Bilański

Strzałka

et al. 2019

Antonie van Leeuwenhoek

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Fungi under the order Ophiostomatales (Ascomycota) are known to associate with various species of bark beetles (Coleoptera: Curculionidae: Scolytinae). In addition this group of fungi contains many taxa that can impart blue-stain on sapwood and some are important tree pathogens. A recent survey that focussed on the diversity of the Ophiostomatales in the forest ecosystems of the Czech Republic and Poland uncovered four putative new species. Phylogenetic analyses of four gene regions (ITS1-5.8S-ITS2 region, ß-tubulin, calmodulin, and translation elongation factor 1-α) indicated that these four species are members of the genus Ophiostoma. All four newly described species can be distinguished from each other and from closely related species based on DNA sequence comparisons, morphological characters, growth rates, and their insect associations. Based on this study four new taxa can be circumscribed and the following names are provided: Ophiostoma pityokteinis sp. nov., Ophiostoma rufum sp. nov., Ophiostoma solheimii sp. nov., and Ophiostoma taphrorychi sp. nov. O. rufum sp. nov. is a member of the Ophiostoma piceae species complex, while O. pityokteinis sp. nov. resides in a discrete lineage within Ophiostoma s. stricto. O. taphrorychi sp. nov. together with O. distortum formed a well-supported clade in Ophiostoma s. stricto close to O. pityokteinis sp. nov. O. solheimii sp. nov. groups within a currently undefined lineage A, which also includes Ophiostoma grandicarpum and Ophiostoma microsporum. This study highlights the need for more intensive surveys that should include additional countries of Central Europe, insect vectors and host tree species in order to elucidate Ophiostoma species diversity in this region.Electronic supplementary materialThe online version of this article (10.1007/s10482-019-01277-5) contains supplementary material, which is available to authorized users.

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Ophiostomatoid fungi and their roles in <i>Quercus robur</i> die-back in Tellermann forest, Russia

Cited by 9 publications

References 26 publications

Seasonal Succession of Fungi Associated with Ips typographus Beetles and Their Phoretic Mites in an Outbreak Region of Finland

Seasonal Succession of Fungi Associated with Ips typographus Beetles and Their Phoretic Mites in an Outbreak Region of Finland

Filamentous fungal associates of the alder bark beetle, Alniphagus aspericollis, including an undescribed species of Neonectria

Four new Ophiostoma species associated with conifer- and hardwood-infesting bark and ambrosia beetles from the Czech Republic and Poland

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