Ophthalmic Pterygium

Chui, Jeanie; Coroneo, Minas T.; Tat, Lien; Crouch, Roger; Wakefield, Denis; Girolamo, Nick Di

doi:10.1016/j.ajpath.2010.10.037

Cited by 236 publications

(140 citation statements)

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“…A previous study also reported a decreased p63 expression in the more superficial cell layers of pterygium, with a staining pattern very similar to the normal sclerocorneal limbus [34]. In a recent study, Chui et al [35] observed in pterygia some morphologically primitive cells resembling limbal stem cells (LSCs) through their co-expression of cytokeratin (CK)-15, CK-19, and p63α. These cells appeared to be localized at the advancing head of the pterygium and they suggested that pterygium was a disease of LSCs, and the presence of these cell clusters may account for the clinical behavior of pterygium.…”

Section: Discussionmentioning

confidence: 99%

Expression of CREB in Primary Pterygium and Correlation with Cyclin D1, ki-67, MMP7, p53, p63, Survivin and Vimentin

et al. 2013

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Aim: Ultraviolet (UV) B irradiation induces gene expression that leads to skin cancer. Among the transcription factors induced by UVB radiation exposure, the cyclic AMP response element-binding protein (CREB) is significant. Since several factors downstream of CREB signaling are known to be involved in pterygium pathogenesis, we investigated CREB expression in pterygial and human conjunctival tissues to evaluate if a similar expression pattern is present. Moreover, we analyzed the correlation with CREB expression and other known pterygium markers. Methods: Primary pterygium samples and normal bulbar conjunctivas surgically removed were analyzed. Formalin-fixed, paraffin-embedded tissues were stained by immunohistochemistry with anti-CREB, anti-vimentin, anti-ki-67, anti-survivin, anti-MMP7, anti-p63, anti-cyclin D1, or anti-p53 antibodies. Results: 94.4% of pterygium samples were positive for CREB with a significant difference compared to the control group (p = 0.002). The staining was localized in the epithelium and absent in the stroma. An increased expression was found for cyclin D1 (p = 0.019), ki-67 (p = 0.005), vimentin (p = 0.003), survivin (p < 0.001), p63 (p = 0.003), and MMP7 (p = 0.002). CREB expression showed a significant correlation with cyclin D1 (ρ = 0.49; p = 0.035), ki-67 (ρ = 0.61; p = 0.007), and p53 (ρ = 0.57; p = 0.013) in pterygium. Conclusions: These results permit to hypothesize that CREB is involved in pterygium pathogenesis. Since various molecules have been discovered to inhibit CREB, these data could be of interest for pterygium treatment.

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Section: Discussionmentioning

confidence: 99%

Expression of CREB in Primary Pterygium and Correlation with Cyclin D1, ki-67, MMP7, p53, p63, Survivin and Vimentin

et al. 2013

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“…(8) y Canadá (17) . Por el contrario, dos estudios realizados en Australia encontraron frecuencias no sospechada del 5 (16) y 9,8% (15) . Es probable que estas investigaciones australianas reportaran frecuencias más altas, porque el estudio histopatológico de los especímenes de pterigión se desarrolló específicamente para identificar la presencia de NESO.…”

Section: Discussionunclassified

“…Es probable que estas investigaciones australianas reportaran frecuencias más altas, porque el estudio histopatológico de los especímenes de pterigión se desarrolló específicamente para identificar la presencia de NESO. Otras razones que pueden explicar la mayor frecuencia en Australia incluyen un índice de radiación UV alto debido a la deficiencia de la capa de ozono en esta región (23,24) , la mayor susceptibilidad de la raza caucásica para desarrollar NESO que está relacionada a la piel blanca y/o iris pálido (23) y posiblemente a factores genéticos (8,(15)(16)(17)25) .…”

Section: Discussionunclassified

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Neoplasia escamosa de la superficie ocular en pacientes con pterigión en Perú

Furuya‐Kanamori

Dulanto-Reinoso

Stone

et al. 2014

Rev Peru Med Exp Salud Publica

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Objetivos. Estimar la frecuencia de neoplasia escamosa de la superficie ocular (NESO) no sospechada en pterigión, la precisión del diagnóstico clínico y las características demográficas y clínicas asociadas. Materiales y métodos. Se examinaron los informes histopatológicos de los pacientes con diagnóstico clínico de pterigión y/o NESO que fueron quirúrgicamente tratados entre marzo de 2009 y diciembre de 2012 en el Instituto Nacional de Oftalmología en Lima, Perú. La precisión del diagnóstico clínico para identificar la NESO se evaluó mediante la sensibilidad, especificidad y los cocientes de probabilidad. Se realizaron modelos de regresión log-log negativos para identificar las características demográficas y clínicas asociadas con un aumento de las probabilidades de diagnosticar NESO. Resultados. Se examinaron 3021 informes de histopatología. La frecuencia de NESO no sospechada en pterigión fue de 0,65%. El diagnóstico clínico presentó una sensibilidad del 85%, una especificidad del 99%, un cociente de probabilidad positiva de 111,89 y un cociente probabilidad negativa de 0,15. Las características asociadas fueron el sexo masculino (OR 1,15; IC 95%:1,01-1,30), pacientes de 61 a 80 años (OR 1,54; IC 95%: 1,28-1,85), ≥ de 81 años (OR 3,10; IC 95%: 2,09-4,58), pacientes con lesiones recurrentes (OR 1,59; IC 95%: 1,03-2,46) y lesiones en el lado temporal (OR 3,57; IC 95%: 2,63-4,85) presentaron mayor probabilidad de NESO. Conclusiones. Se encontró una baja frecuencia de NESO no sospechada, sin embargo, es recomendable realizar el estudio histopatológico de forma rutinaria para evitar diagnósticos erróneos de NESO como pterigión. Palabras clave: Pterigion; Neoplasias oculares; Diagnóstico clínico (fuente: DeCS BIREME). SQUAMOUS NEOPLASIA OF THE OCULAR SURFACE IN PATIENTS WITH PTERYGIUM IN PERU ABSTRACTObjectives. To estimate the frequency of unsuspected ocular surface squamous neoplasia (OSSN) in pterygium, the accuracy of clinical diagnosis, and associated demographic and clinical characteristics. Materials and methods. We reviewed histopathological reports of patients with a clinical diagnosis of pterygium and/or OSSN who were surgically treated between March 2009 and December 2012 at the National Eye Institute in Lima, Peru. The accuracy of the clinical diagnosis of OSSN was assessed by sensitivity, specificity, and likelihood ratios. Models of negative log-log regression were performed to identify demographic and clinical characteristics associated with increased odds of diagnosing OSSN. Results. 3,021 histopathological reports were reviewed. The frequency of unsuspected OSSN in pterygium was 0.65%. Clinical diagnosis had a sensitivity of 85%, a specificity of 99%, a positive likelihood ratio of 111.89, and a negative likelihood ratio of 0.15. Associated characteristics were male gender (OR =1.15; 95% CI: 1.01 to 1.30), age group of 61-80 years (OR = 1.54, 95% CI: 1.28 to 1.85) ≥ 81 years (OR = 3.10; 95% CI: 2.09 to 4.58), presence of recurrent lesions (OR = 1.59; 95% CI: 1.03 to 2.46) and temporal location lesi...

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“…We showed here that basal cells had a higher VEGF mRNA amplification score compared with epithelial cells from the upper layers of the pterygium epithelium. High VEGF mRNA levels detected on the basal cells could be explained by their particular phenotype and behavior described by Chui et al [19], who suggested that basal cells could have a premalignant features. One of our previous studies concerning the presence and distribution of thymine dimers inside pterygium epithelium showed that basal cells are characterized by the highest expression of thymine dimers, which was also associated with a high density of p53 mutant basal cells [20].…”

Section: Discussionmentioning

confidence: 99%

VEGF mRNA Assessment in Human Pterygium: A New ‘Scope' for a Future Hope

2014

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Purpose: The lack of powerful evidence to support the efficacy of anti-vascular endothelial growth factor (VEGF) therapy in human pterygium can be attributed to incomplete VEGF expression assessment by restrictive use of immunohistochemistry only and failure to use the molecular methods able to confirm immunohistochemical findings. By adding at least one more sensitive method to assess human pterygium VEGF expression, a more accurate selection of patients for bevacizumab therapy could be done and this would improve the efficacy of anti-VEGF therapy in human pterygium. Methods: We assessed VEGF mRNA amplification on paraffin-embedded specimens by applying the RNAscope method for the first time in human pterygium, an in situ hybridization-based technique able to detect VEGF mRNA as a single gene copy on paraffin-embedded samples. Results: Heterogeneous VEGF mRNA distribution and amplification inside the epithelial compartment of human pterygium were observed. Despite previous reports concerning the immunohistochemical expression of VEGF in the human pterygium fibrovascular compartment, no stromal components were characterized by VEGF mRNA amplification assessed by in situ hybridization in our study. A higher amplification score was observed in epithelium from recurrent pterygium, especially located in the basal and suprabasal epithelial cells. Conclusions: Based on our findings we consider that in situ hybridization assessment of VEGF for human pterygium specimens can be a useful tool for reconsidering the selection of pterygium patients to be enrolled in anti-VEGF therapy.

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Ophthalmic Pterygium

Cited by 236 publications

References 62 publications

Expression of CREB in Primary Pterygium and Correlation with Cyclin D1, ki-67, MMP7, p53, p63, Survivin and Vimentin

Expression of CREB in Primary Pterygium and Correlation with Cyclin D1, ki-67, MMP7, p53, p63, Survivin and Vimentin

Neoplasia escamosa de la superficie ocular en pacientes con pterigión en Perú

VEGF mRNA Assessment in Human Pterygium: A New ‘Scope' for a Future Hope

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