Opioid Peptides Determination by Tyrosinase-Modified Oxygen Electrode

Eremenko, A. V.; Barmin, A. V.; Kurochkin, Ilya N.

doi:10.1080/00032719508000373

Cited by 5 publications

(2 citation statements)

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“…Figure 4B shows the current-time recording of the bienzyme electrode response on stepwise additions (0.5 pM) of tyrosine (a) and morphiceptin (b). The calculated detection limit (SIN = 3) for DSLET, Leuenkephalin, DADLE and morphiceptin was 0.2 pM, that is 30-80 times lower as described earlier for DADLE, morphiceptin and DTLET [2]. The reproducibility of the bienzyme electrode is 4.3% (n = 5; 0.53 2 0.03 nA).…”

Section: Characterization Of the Bienzyme Electrodesupporting

confidence: 51%

“…For example, enkephalines and endorphines are peptides with high affinity to opioid receptors, and they exhibit very strong morphine-like analgetic properties [ 11. The opioid peptides containing a tyrosine residue in N-terminal position can be detected using a tyrosinasemodified Clark-type oxygen electrode with usual sensitivity for monoenzyme electrodes [2]. The detection limit of this elecrode for DADLE, morphiceptin and DTLET was 6 pM, 9 p M and 16 pM, respectively.…”

Section: Introductionmentioning

confidence: 99%

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A bienzyme electrode for tyrosine‐containing peptides determination

et al. 1997

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A bienzyme electrode for monitoring biologically important peptides containing tyrosine has been established on the basis of mushroom tyrosinase and quinoprotein glucose dehydrogenase (GDH). Tyrosine residues bound in the peptide chain are oxidized by tyrosinase resulting in consumption of oxygen. Subsequently, the dopaquinone residues are reduced in the GDH catalysed reaction which is driven by an excess of glucose. This reaction cycle leads to a considerable increase of sensitivity. Both enzymes were entraped in poly(viny1)alcohol matrix and placed on the surface of a Clark-type oxygen electrode (the working platinum electrode was potentiostated at -600 mV (vs. Ag/AgCl reference electrode) between a polypropylene and cellulose membrane. The bienzyme-modified Clark-type oxygen electrode has a lower detection limit of 0.2 pM for the opioid peptides Tyr-o-Ser-Gly-Phe-Leu-Thr, Leu-enkephalin, Tyr-o-Ala-Gly-Phe-o-Leu and morphiceptin. The dependence of response of the electrode on the length of peptide chain and position of tyrosine residue is discussed. The new electrode has been applied to the quality control of tyrosine containing peptides from pharmaceutical formulations and from peptide synthesis.

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Section: Characterization Of the Bienzyme Electrodesupporting

confidence: 51%

Section: Introductionmentioning

confidence: 99%