Opsonic function of sialic acid specific lectin in freshwater crab Paratelphusa jacquemontii

Abstract

The sialic acid specific humoral lectin, Pjlec of the freshwater crab Paratelphusa jacquemontii was investigated for its opsonin function with rabbit erythrocyte as target cell for phagocytosis by the crab’s hemocyte. The untreated or trypsin treated erythrocyte induced lectin response after challenge however failed when treated with neuraminidase evidently indicating glycan dependency for elicited immune response. Our observation of in vitro phagocytosis of the erythrocyte untreated or coated with serum, clarified serum appeared to be recognized and engulfed by hemocytes but when coated with isolated lectin Pjlec, the response was elicited. Moreover, with trypsin treated erythrocyte the response remained unchanged but neuraminidase or O-glycosidase treatment eliminated the response reaction. This suggested the sialic acid specific reaction of lectin with the erythrocyte and was essential for recognition to allow the lectin Pjlec to act as an opsonin. The flowcytometry observation affirmed the enhancement of phagocytosis by Pjlec coated hemocyte. The efficiency of in vitro hemolysis of Pjlec coated erythrocyte with hemocyte when compared to untreated erythrocyte with or without hemocyte also established the opsonic function of the lectin. The mechanism of phagocytosis and induction were dependent on specific recognition of the erythrocyte by the multivalent binding site of the lectin protein, and the elicitation of the immune response was a function of the sialoglycan surface. The pathway of the challenge suggested that after entry of nonself recognition by lectin was followed by induction and activation of phagocytosis by opsonic binding of the lectin.

N‐acetyl‐D ‐glucosamine‐binding lectin ( PrLec ) from the serum of healthy blue swimming crab Portunus reticulatus agglutinates Gram‐positive ( Micrococcus luteus ) and Gram‐negative ( Escherichia coli ) bacteria

“…Our results show that the PrLec from the haemolymph of the swimmer crab P. reticulatus was able to agglutinate E. coli effectively, which can be further studied in detail to identify the action mechanism. The lectin from freshwater crab P. jacquemontii was observed to have opsonic activity that leads to effective clearance of nonself-molecules (Denis et al, 2015). Works carried out by Tasumi and Vasta (2007) showed the role of haemocyte associated lectin from Litopenaeus vennaamei displayed broad antimicrobial activity towards Gram-positive and Gram-negative bacteria and was also observed to act as an opsonin (Cha et al, 2015).…”

“…The lectin purified in our study from P. reticulatus was performed by affinity chromatography using chitosan beads. Affinity columns have been majorly used for purification of lectins from crabs and one such example is the lectin purified using bovine submaxillary mucin (BSM)‐activated Sepharose affinity chromatography from freshwater crab P. jacquemontii and lectin from marine crab, C. antennarius showing greater affinity to BSM was purified through affinity chromatography (Denis et al, 2015; Ravindranath et al, 1985). Chitin based affinity column was efficiently used in isolating N‐acetyl‐D‐glucosamine (GlcNac) binding lectin by Kochibe and Matta (1989).…”

Efficiency of lectins as immune indicators in juvenile white shrimp, Litopenaeus vannamei (Malacostraca: Penaeidae)

“…En la inmunoelectrotransferencia se identificó que los anticuerpos pueden reconocer 3 (80, 61, 48 kDa) de las 4 fracciones de lectina purificada de L. vannamei, lo cual implica un reconocimiento del 75% de lectina presente en la hemolinfa. Es probable que la lectina de M. rosenbergii y L. vannamei compartan epítopes comunes, debido a que las lectinas de estos crustáceos se consideran evolutivamente bien conservadas (Pereyra et al, 2009;Luo et al, 2011;Denis et al, 2016). Adicionalmente, en un estudio realizado por Pereyra et al (2012), anticuerpos monoclonales anti MrL de M. rosenbergii detectaron dos subunidades de lectina (42.7 y 66.2 kDa) de Macrobrachium americanum (Bate, 1868), de las 3 subunidades previamente purificadas (86.6, 42.7 y 66.2 kDa), lo cual demuestra que el anticuerpo reconoce epítopes conservados en lectinas de ambas especies.…”

Reconstruction of Eriocheir sinensis Y-organ Genome-Scale Metabolic Network and Differential Analysis After Eyestalk Ablation

“…Among the crustaceans, sialic acid lectin has been extracted from Homarus americanus (Hall and Rowlands, 1974), Macrobrachium rosenbergii (Vasta et al, 1983), Cancer antennarius (Ravindranath et al, 1985), Scylla serrate (Mercy and Ravindranath, 1993), and Penaeus monodon (Ratanapo, 1990). Denis et al (2015) extracted sialic acid-specific lectin from blood lymphocytes of Paratelphusa jacquemontii using bovine submandibular gland mucus as an affinity chromatography ligand. In the GSMN of E. sinensis eyestalk, we found the synthetic reactions of sialic acid from N-acetyl-D-glucosamine-6-phosphate, which suggests that there are sialic acid synthesis pathway and corresponding functions in E. sinensis.…”