Reconstruction of Eriocheir sinensis Y-organ Genome-Scale Metabolic Network and Differential Analysis After Eyestalk Ablation

Wang, Bin; Yang, Jiarui; Gao, Chenchen; Hao, Tong; Li, Jingjing; Sun, Jinsheng

doi:10.3389/fgene.2020.532492

Cited by 4 publications

(4 citation statements)

References 61 publications

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“…Serotonin, dopamine, and octopamine function as neurotransmitters and neuromodulators in the crustacean central nervous system, but they may also act as neurohormones (46,60,(88)(89)(90)(91). Interestingly, there is evidence that the YO can synthesize serotonin (92). Much of the research of biogenic amines functioning as neurohormones has been focused on their roles in regulating decapod reproduction.…”

Section: Ca 2+ /Diacylglycerol/protein Kinase C Signalingmentioning

confidence: 99%

Signaling Pathways That Regulate the Crustacean Molting Gland

Mykles

2021

Front. Endocrinol.

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A pair of Y-organs (YOs) are the molting glands of decapod crustaceans. They synthesize and secrete steroid molting hormones (ecdysteroids) and their activity is controlled by external and internal signals. The YO transitions through four physiological states over the molt cycle, which are mediated by molt-inhibiting hormone (MIH; basal state), mechanistic Target of Rapamycin Complex 1 (mTORC1; activated state), Transforming Growth Factor-β (TGFβ)/Activin (committed state), and ecdysteroid (repressed state) signaling pathways. MIH, produced in the eyestalk X-organ/sinus gland complex, inhibits the synthesis of ecdysteroids. A model for MIH signaling is organized into a cAMP/Ca2+-dependent triggering phase and a nitric oxide/cGMP-dependent summation phase, which maintains the YO in the basal state during intermolt. A reduction in MIH release triggers YO activation, which requires mTORC1-dependent protein synthesis, followed by mTORC1-dependent gene expression. TGFβ/Activin signaling is required for YO commitment in mid-premolt. The YO transcriptome has 878 unique contigs assigned to 23 KEGG signaling pathways, 478 of which are differentially expressed over the molt cycle. Ninety-nine contigs encode G protein-coupled receptors (GPCRs), 65 of which bind a variety of neuropeptides and biogenic amines. Among these are putative receptors for MIH/crustacean hyperglycemic hormone neuropeptides, corazonin, relaxin, serotonin, octopamine, dopamine, allatostatins, Bursicon, ecdysis-triggering hormone (ETH), CCHamide, FMRFamide, and proctolin. Contigs encoding receptor tyrosine kinase insulin-like receptor, epidermal growth factor (EGF) receptor, and fibroblast growth factor (FGF) receptor and ligands EGF and FGF suggest that the YO is positively regulated by insulin-like peptides and growth factors. Future research should focus on the interactions of signaling pathways that integrate physiological status with environmental cues for molt control.

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Section: Ca 2+ /Diacylglycerol/protein Kinase C Signalingmentioning

confidence: 99%

Signaling Pathways That Regulate the Crustacean Molting Gland

Mykles

2021

Front. Endocrinol.

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“…If multiple genes correspond to one reaction, intergenic relationships need to be determined. When the genes of a reaction correspond to different enzymes, their relationships are considered “or,” and if several genes encode the same enzyme, their relationships are considered “and” ( Wang et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Reconstruction of Litopenaeus vannamei Genome-Scale Metabolic Network Model and Nutritional Requirements Analysis of Different Shrimp Commercial Varieties

et al. 2021

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As an important tool for systematic analysis, genome-scale metabolic network (GSMN) model has been widely used in various organisms. However, there are few reports on the GSMNs of aquatic crustaceans. Litopenaeus vannamei is the largest and most productive shrimp species. Feed improvement is one of the important methods to improve the yield of L. vannamei and control water pollution caused by the inadequate absorption of feed. In this work, the first L. vannamei GSMN named iGH3005 was reconstructed and applied to the optimization of feed. iGH3005 was reconstructed based on the genomic data. The model includes 2,292 reactions and 3,005 genes. iGH3005 was used to analyze the nutritional requirements of five different L. vannamei commercial varieties and the genes influencing the metabolism of the nutrients. Based on the simulation, we found that tyrosine-protein kinase src64b like may catalyze different reactions in different commercial varieties. The preference of carbohydrate utilization is different in various commercial varieties, which may due to the different expressions of some genes. In addition, this investigation suggests that a rational and targeted modification in the macronutrient content of shrimp feed would lead to an increase in growth and feed conversion rate. The feed for different commercial varieties should be adjusted accordingly, and possible adjustment schemes were provided. The results of this work provided important information for physiological research and optimization of the components in feed of L. vannamei.

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“…sinensis, commonly known as river crab or Chinese mitten crab, belongs to the phylum Arthropoda. E. sinensis is an important economic source of fishery [17]. Molting is an important characteristic of the growth of E. sinensis.…”

Section: Introductionmentioning

confidence: 99%

Reconstruction of Metabolic–Protein Interaction Integrated Network of Eriocheir sinensis and Analysis of Ecdysone Synthesis

Hao,

Song,

Zhang

et al. 2024

Genes

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Integrated networks have become a new interest in genome-scale network research due to their ability to comprehensively reflect and analyze the molecular processes in cells. Currently, none of the integrated networks have been reported for higher organisms. Eriocheir sinensis is a typical aquatic animal that grows through ecdysis. Ecdysone has been identified to be a crucial regulator of ecdysis, but the influence factors and regulatory mechanisms of ecdysone synthesis in E. sinensis are still unclear. In this work, the genome-scale metabolic network and protein–protein interaction network of E. sinensis were integrated to reconstruct a metabolic–protein interaction integrated network (MPIN). The MPIN was used to analyze the influence factors of ecdysone synthesis through flux variation analysis. In total, 236 integrated reactions (IRs) were found to influence the ecdysone synthesis of which 16 IRs had a significant impact. These IRs constitute three ecdysone synthesis routes. It is found that there might be alternative pathways to obtain cholesterol for ecdysone synthesis in E. sinensis instead of absorbing it directly from the feeds. The MPIN reconstructed in this work is the first integrated network for higher organisms. The analysis based on the MPIN supplies important information for the mechanism analysis of ecdysone synthesis in E. sinensis.

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Reconstruction of Eriocheir sinensis Y-organ Genome-Scale Metabolic Network and Differential Analysis After Eyestalk Ablation

Cited by 4 publications

References 61 publications

Signaling Pathways That Regulate the Crustacean Molting Gland

Signaling Pathways That Regulate the Crustacean Molting Gland

Reconstruction of Litopenaeus vannamei Genome-Scale Metabolic Network Model and Nutritional Requirements Analysis of Different Shrimp Commercial Varieties

Reconstruction of Metabolic–Protein Interaction Integrated Network of Eriocheir sinensis and Analysis of Ecdysone Synthesis

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