Optical absorption spectra of deoxy- and oxyhemoglobin in the temperature range 300—20 K

Cordone, Lorenzo; Cupane, Antonio; Leone, Maurizio; Vitrano, Eugenio

doi:10.1016/0301-4622(86)85031-1

Cited by 91 publications

(94 citation statements)

References 22 publications

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“…The base line (cuvette ϩ solvent) was measured at room temperature and subtracted from each spectrum (in this spectral range, the base line does not depend on temperature, and absorption due to dithionite is negligible); moreover, we stress that our samples remained homogeneous and transparent at all temperatures. All other experimental details were as described previously (16,17).…”

Section: Methodsmentioning

confidence: 99%

Modification of α-Chain or β-Chain Heme Pocket Polarity by Val(E11) → Thr Substitution Has Different Effects on the Steric, Dynamic, and Functional Properties of Human Recombinant Hemoglobin

Cupane

Leone

Militello

et al. 1997

Journal of Biological Chemistry

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The dynamic and functional properties of mutant deoxyhemoglobins in which either the ␤-globin Val 67 (E11) or the ␣-globin Val 62 (E11) is replaced by threonine have been investigated through the thermal evolution of the Soret absorption band in the temperature range 300 to 20 K and through the kinetics of CO rebinding after flash photolysis at room temperature. The conformational properties of the modified ␣ chain and ␤ chain distal heme pockets were also studied through x-ray crystallography and molecular modeling. The data obtained with the various techniques consistently indicate that the polar isosteric mutation in the distal side of the ␣ chain heme pocket has a larger effect on the investigated properties than the analogous mutation on the ␤ chain. We attribute the observed differences to the presence of a water molecule in the distal heme pocket of the modified ␣ chains, interacting with the hydroxyl of the threonine side chain. This is indicated by molecular modeling which showed that the water molecule present in the ␣ chain distal heme pocket can bridge by H bonding between Thr 62 (E11) and His 58 (E7) without introducing any unfavorable steric interactions. Consistent with the dynamic and functional data, the presence of a water molecule in the distal heme pocket of the modified ␤ chains is not observed by x-ray crystallography.

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Section: Methodsmentioning

confidence: 99%

Modification of α-Chain or β-Chain Heme Pocket Polarity by Val(E11) → Thr Substitution Has Different Effects on the Steric, Dynamic, and Functional Properties of Human Recombinant Hemoglobin

Cupane

Leone

Militello

et al. 1997

Journal of Biological Chemistry

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“…24), which was also reported to be independent of oxygenation status 25 and temperature. 26,27 The intracellular concentration of hemoglobin is a part of broad homeostasis and is relatively constant for individual species. For example, for adult humans, it varies in the range of 330-360 mg/ml or 5.1-5.6 mM.…”

mentioning

confidence: 99%

“…28 Temperature changes affect the equilibrium between oxygenated/deoxygenated forms of hemoglobin, leading to changes in optical absorption coefficient everywhere but around its isosbestic wavelengths. 26,27 The isosbestic point of hemoglobin at 805 nm is the only one acceptable for deep live tissue temperature monitoring applications, since it is located within the near-infrared spectral transparency window.…”

mentioning

confidence: 99%

Red blood cell as a universal optoacoustic sensor for non-invasive temperature monitoring

Петрова

Oraevsky

Ermilov

2014

Applied Physics Letters

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Optoacoustic (photoacoustic) temperature imaging could provide improved spatial resolution and temperature sensitivity as compared to other techniques of non-invasive thermometry used during thermal therapies for safe and efficient treatment of lesions. However, accuracy of the reported optoacoustic methods is compromised by biological variability and heterogeneous composition of tissues. We report our findings on the universal character of the normalized temperature dependent optoacoustic response (ThOR) in blood, which is invariant with respect to hematocrit at the isosbestic point of hemoglobin. The phenomenon is caused by the unique homeostatic compartmentalization of blood hemoglobin exclusively inside erythrocytes. On the contrary, the normalized ThOR in aqueous solutions of hemoglobin showed linear variation with respect to its concentration and was identical to that of blood when extrapolated to the hemoglobin concentration inside erythrocytes. To substantiate the conclusions, we analyzed optoacoustic images acquired from the samples of whole and diluted blood as well as hemoglobin solutions during gradual cooling from þ37 to À15 C. Our experimental methodology allowed direct observation and accurate measurement of the temperature of zero optoacoustic response, manifested as the sample's image faded into background and then reappeared in the reversed (negative) contrast. These findings provide a framework necessary for accurate correlation of measured normalized optoacoustic image intensity and local temperature in vascularized tissues independent of tissue composition. Temperature monitoring of live tissue is an important technique that provides feedback information needed for guidance of thermal therapies, such as laser interstitial thermotherapy, high-intensity focused ultrasound, radio frequency ablation, and cryoablation. 1-3 Temperature readings collected in real time around the treated areas help warranting safety and efficiency of the therapeutic procedure. 4 Noninvasive temperature monitoring is unanimously preferred among the clinicians, especially when there are crucial requirements to minimize collateral thermal damage to the adjacent normal tissues and zones of innervation. Several imaging and sensing modalities have been proposed for noninvasive temperature monitoring during thermal therapies, including ultrasound imaging, magnetic resonance imaging, and optoacoustic (photoacoustic) imaging and sensing. [5][6][7] The potential advantages of optoacoustic temperature imaging techniques reside in enhanced spatial resolution, temperature sensitivity, and faster data acquisition. [8][9][10] During the past decade, optoacoustic imaging has attracted significant attention from researchers and clinicians due to its success in a variety of biomedical applications that involve high resolution deep tissue imaging of optical absorbers (blood, water, and near infrared contrast agents) unattainable with other modalities. [11][12][13] The premium quality of optoacoustic images, as compared to other imagin...

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“…Blood is the main absorber in the visible and near IR range of optical radiation and hemoglobin is known to transform at higher temperature into methemoglobin, which induces the change of optical properties of tissue. [33][34][35][36] In this study, using ex vivo liver tissue, the dynamics of blood perfusion was not considered. In future studies, blood perfusion will be considered in the simulation and direct measurement in vivo will be used to calibrate the proposed device and method.…”

Section: Discussionmentioning

confidence: 99%

Interstitial photoacoustic technique and computational simulation for temperature distribution and tissue optical properties in interstitial laser photothermal interaction

Chen

Zhou

et al. 2017

J. Innov. Opt. Health Sci.

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Interstitial laser immunotherapy (ILIT) is designed to use photothermal and immunological interactions for treatment of metastatic cancers. The photothermal e®ect is crucial in inducing anti-tumor immune responses in the host. Tissue temperature and tissue optical properties are important factors in this process. In this study, a device combining interstitial photoacoustic (PA) technique and interstitial laser photothermal interaction is proposed. Together with computational simulation, this device was designed to determine temperature distributions and tissue optical properties during laser treatment. Experiments were performed using ex-vivo porcine liver tissue. Our results demonstrated that interstitial PA signal amplitude was linearly dependent on tissue temperature in the temperature ranges of 20-60 C, as well as 65-80 C, with a di®erent slope, due to the change of tissue optical properties. Using the directly measured temperature in the tissue around the interstitial optical¯ber di®usion tip for calibration, the theoretical temperature distribution predicted by the bioheat equation was used to extract optical properties of tissue. Finally, the three-dimensional temperature distribution was simulated to guide tumor destruction and immunological stimulation. Thus, this novel device and method could be used for monitoring and controlling ILIT for cancer treatment.

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Optical absorption spectra of deoxy- and oxyhemoglobin in the temperature range 300—20 K

Cited by 91 publications

References 22 publications

Modification of α-Chain or β-Chain Heme Pocket Polarity by Val(E11) → Thr Substitution Has Different Effects on the Steric, Dynamic, and Functional Properties of Human Recombinant Hemoglobin

Modification of α-Chain or β-Chain Heme Pocket Polarity by Val(E11) → Thr Substitution Has Different Effects on the Steric, Dynamic, and Functional Properties of Human Recombinant Hemoglobin

Red blood cell as a universal optoacoustic sensor for non-invasive temperature monitoring

Interstitial photoacoustic technique and computational simulation for temperature distribution and tissue optical properties in interstitial laser photothermal interaction

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