Optical characterization of red blood cells from individuals with sickle cell trait and disease in Tanzania using quantitative phase imaging

Jung, Jin‐Woo; Matemba, Lucas E.; Lee, KyeoReh; Kazyoba, Paul; Yoon, Jonghee; Massaga, Julius J.; K, Kim; Kim, Dong-Jin; Park, YongKeun

doi:10.1038/srep31698

Cited by 39 publications

(32 citation statements)

References 61 publications

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“…Based on the principle of holography, QPI techniques exploits the refractive index (RI) distribution of optical contrasts to provide quantitative and label-free images of live cells and tissues. QPI techniques have been applied to study the pathophysiology of various biological samples, including hematology (10), malaria infection (11,12), sickle cell diseases (13)(14)(15)(16), and microbiology (17,18). In particular, 2D QPI techniques or digital holographic microscopy techniques had been utilized for the study of biophysics of neuron cells (19,20) and for imaging alterations in brain tissue slices caused by Alzheimer's disease (21).…”

Section: Introductionmentioning

confidence: 99%

Measurements of morphological and biophysical alterations in individual neuron cells associated with early neurotoxic effects in Parkinson's disease

Yang

Yoon

et al. 2017

Cytometry Pt A

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Parkinson's disease (PD) is a common neurodegenerative disease. However, therapeutic methods of PD are still limited due to complex pathophysiology in PD. Here, optical measurements of individual neurons from in vitro PD model using optical diffraction tomography (ODT) are presented. By measuring 3D refractive index distribution of neurons, morphological and biophysical alterations in in-vitro PD model are quantitatively investigated. It was found that neurons show apoptotic features in early PD progression. The present approach will open up new opportunities for quantitative investigation of the pathophysiology of various neurodegenerative diseases. © 2017 International Society for Advancement of Cytometry.

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Section: Introductionmentioning

confidence: 99%

Measurements of morphological and biophysical alterations in individual neuron cells associated with early neurotoxic effects in Parkinson's disease

Yang

Yoon

et al. 2017

Cytometry Pt A

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“…As a scheme for three-dimensional (3D) QPI, optical diffraction tomography (ODT) is an imaging method that uses angularly varying illumination to reconstruct the 3D refractive index (RI) distribution of a microscopic sample. Since RI, an intrinsic optical property of a sample, provides morphological and biochemical information, ODT has been successfully applied to various fields, including histopathology [2][3][4], hematology [5][6][7][8] microbiology [9], cell biology [10][11][12] and nanotechnology [13].…”

Section: Introductionmentioning

confidence: 99%

Cycle-consistent deep learning approach to coherent noise reduction in optical diffraction tomography

Choi¹,

Ryu

Jo³

et al. 2019

Opt. Express

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We present a deep neural network to reduce coherent noise in three-dimensional quantitative phase imaging. Inspired by the cycle generative adversarial network, the denoising network was trained to learn a transform between two image domains: clean and noisy refractive index tomograms. The unique feature of this network, distinct from previous machine learning approaches employed in the optical imaging problem, is that it uses unpaired images. The learned network quantitatively demonstrated its performance and generalization capability through denoising experiments of various samples. We concluded by applying our technique to reduce the temporally changing noise emerging from focal drift in time-lapse imaging of biological cells. This reduction cannot be performed using other optical methods for denoising.

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“…The western and southern region of the Kingdom of Saudi Arabia has very high incidence of SCD (2%) and SCT (15%) [ 3 ]. The most common conventional method of screening for any inherited blood disorder is clinical examination followed by CBC and electrophoresis [ 4 – 6 ], or occasionally HPLC [ 7 ]. Out of these, electrophoresis is based on the difference in the movement of different hemoglobins, under the influence of a given electric field, and HPLC is based on the movement of different hemoglobins in a given gravitational field, as they pass through certain adhesive materials.…”

Section: Introductionmentioning

confidence: 99%

A Novel Technique of Spectral Discrimination of Variants of Sickle Cell Anemia

et al. 2018

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Sickle cell anemia (SCA) is an inherited blood disorder with worldwide incidence of 15%; out of this, it is found in up to 20% in countries like Kingdom of Saudi Arabia and Bahrain. The standard conventional method of detection is complete blood count (CBC) followed by hemoglobin electrophoresis or high-performance liquid chromatography (HPLC) or both. In this context, spectral detection of variants of sickle cell anemia (SCA) is an innovative technique, which when made accurate and reliable could be an effective alternative, since the instrumentation is compact (5 kg) and hence portable. This makes mass screening even in remote villages possible. In this paper, we give the essential aspects of fluorescent spectral features of sickle cell trait (SCT), sickle cell disease (SCD), beta (β) thalassemia trait (BTT) + SCD, and beta (β) thalassemia disease (BTD) + SCD. All the above four major variants could be discriminated among themselves and also from the normal control blood sample. All these analyses could be carried out with 5 ml of blood, in a time period of 10 minutes. The results of this paper give strong support for an alternative method, a spectral technique, for molecular-level diagnosis of sickle cell anemia and other closely related blood disorders.

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Optical characterization of red blood cells from individuals with sickle cell trait and disease in Tanzania using quantitative phase imaging

Cited by 39 publications

References 61 publications

Measurements of morphological and biophysical alterations in individual neuron cells associated with early neurotoxic effects in Parkinson's disease

Measurements of morphological and biophysical alterations in individual neuron cells associated with early neurotoxic effects in Parkinson's disease

Cycle-consistent deep learning approach to coherent noise reduction in optical diffraction tomography

A Novel Technique of Spectral Discrimination of Variants of Sickle Cell Anemia

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