Optical Clearing for OCT Image Enhancement and In-Depth Monitoring of Molecular Diffusion

Larin, Kirill V.; Ghosn, Mohamad G.; Bashkatov, Alexey N.; Genina, Elina A.; Trunina, N. A.; Tuchin, Valery V.

doi:10.1109/jstqe.2011.2181991

Cited by 97 publications

(80 citation statements)

References 102 publications

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“…1,[10][11][12][13][14][15] Recent research has demonstrated that this technique is completely reversible and can be used in vivo in cooperation with imaging methods. Some examples are speckle methods to monitor blood°ow in dermis or cortical tissues, 16 optical coherence tomography (OCT) 17 or second harmonic generation (SHG) imaging to improve tissue depth and resolution. 18 As already indicated above, the two main mechanisms of optical clearing (OC) are designated as tissue dehydration and RI matching.…”

Section: Introduction and Basic Conceptmentioning

confidence: 99%

Optical clearing mechanisms characterization in muscle

Oliveira

Carvalho

Nogueira

et al. 2016

J. Innov. Opt. Health Sci.

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Optical immersion clearing is a technique that has been widely studied for more than two decades and that is used to originate a temporary transparency effect in biological tissues. If applied in cooperation with clinical methods it provides optimization of diagnosis and treatment procedures. This technique turns biological tissues more transparent through two main mechanisms — tissue dehydration and refractive index (RI) matching between tissue components. Such matching is obtained by partial replacement of interstitial water by a biocompatible agent that presents higher RI and it can be completely reversible by natural rehydration in vivo or by assisted rehydration in ex vivo tissues. Experimental data to characterize and discriminate between the two mechanisms and to find new ones are necessary. Using a simple method, based on collimated transmittance and thickness measurements made from muscle samples under treatment, we have estimated the diffusion properties of glucose, ethylene glycol (EG) and water that were used to perform such characterization and discrimination. Comparing these properties with data from literature that characterize their diffusion in water we have observed that muscle cell membrane permeability limits agent and water diffusion in the muscle. The same experimental data has allowed to calculate the optical clearing (OC) efficiency and make an interpretation of the internal changes that occurred in muscle during the treatments. The same methodology can now be used to perform similar studies with other agents and in other tissues in order to solve engineering problems at design of inexpensive and robust technologies for a considerable improvement of optical tomographic techniques with better contrast and in-depth imaging.

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Section: Introduction and Basic Conceptmentioning

confidence: 99%

Optical clearing mechanisms characterization in muscle

Oliveira

Carvalho

Nogueira

et al. 2016

J. Innov. Opt. Health Sci.

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“…However, the turbid skin-induced static speckles conceals the dynamic information of the blood flow and reduces the imaging resolution and contrast, that makes it difficult to sensitively monitor the cutaneous blood flow dynamical response [23]. Fortunately, the tissue optical clearing technique [24][25][26] has shown a great potential to improve the performance of many optical imaging modalities [27][28][29][30][31][32][33][34][35][36]. Especially, some in vivo skin optical clearing (SOC) methods [37,38] can make LSCI image the cutaneous microcirculation with superior resolution and contrast [39][40][41].…”

Section: Introductionmentioning

confidence: 99%

Accessing to arteriovenous blood flow dynamics response using combined laser speckle contrast imaging and skin optical clearing

Shi¹,

Chen²,

Tuchin³

et al. 2015

Biomed. Opt. Express

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Laser speckle contrast imaging (LSCI) shows a great potential for monitoring blood flow, but the spatial resolution suffers from the scattering of tissue. Here, we demonstrate the capability of a combination method of LSCI and skin optical clearing to describe in detail the dynamic response of cutaneous vasculature to vasoactive noradrenaline injection. Moreover, the superior resolution, contrast and sensitivity make it possible to rebuild arteries-veins separation and quantitatively assess the blood flow dynamical changes in terms of flow velocity and vascular diameter at single artery or vein level.

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“…The temporary selective optical clearing of tissue layers is the key technique for structural and functional imaging, particularly for detection of local morphological or physiological inhomogeneities hidden within a highly scattering medium. 3,4,[11][12][13][14][15][16][17] The optical clearing of biological tissue is based on partial replacement of tissue interstitial°uid by immersion agent with refractive index higher than the refractive index of interstitial°u id and tissue dehydration. These molecular processes, related to phenomenon of osmoses, lead to the matching of refractive indices of the interstitial°u id and tissue structures (collagen¯bers, cell organelles, etc.…”

Section: Introductionmentioning

confidence: 99%

Quantification of glucose and glycerol diffusion in myocardium

Tuchina

Bashkatov

Genina

et al. 2015

J. Innov. Opt. Health Sci.

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The results on determination of glucose and glycerol di®usion coe±cients in myocardium tissue are presented. The method is based on the measurement and analysis of temporal dependence of tissue optical collimated transmittance under action of a hyperosmotic agent. This temporal tissue response is related to the rate of the agent and water di®usion in a tissue. The di®usion coe±cients for tissue°uid°uxes at glucose and glycerol application to the myocardium at 20 C have been estimated as ð4:75 AE 3:40Þ Â 10 À7 and ð7:71 AE 4:63Þ Â 10 À7 cm 2 /s, respectively.

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Optical Clearing for OCT Image Enhancement and In-Depth Monitoring of Molecular Diffusion

Cited by 97 publications

References 102 publications

Optical clearing mechanisms characterization in muscle

Optical clearing mechanisms characterization in muscle

Accessing to arteriovenous blood flow dynamics response using combined laser speckle contrast imaging and skin optical clearing

Quantification of glucose and glycerol diffusion in myocardium

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