OPTICAL COHERENCE TOMOGRAPHY OF ADIPOSE TISSUE AT PHOTODYNAMIC/PHOTOTHERMAL TREATMENT IN VITRO

Abstract

Temporal changes in structure and refractive-index distribution of adipose tissue at photodynamic/photothermal treatment were studied with OCT in vitro. EthanolÀwater solutions of indocyanine green (ICG) and brilliant green (BG) were used for fat tissue staining. CW laser diode (808 nm) and LED light source (442 and 597 nm) were used for irradiation of stained tissue slices. The data received supporting the hypothesis that photodynamic/photothermal treatment, induces fat cell lipolysis during a certain period of time after light exposure.

Monitoring of temperature-mediated phase transitions of adipose tissue by combined optical coherence tomography and Abbe refractometry

“…This operation smooths out the random noise in the system and influence of inhomogeneity of cell structure of the tissue, while the peaks corresponding to the sample boundaries become more distinct. 9,10 Processing and smoothing of OCT signals and determination of distance between peaks were performed using MathCAD (PTC, Inc.). The "medsmooth" function was used for smoothing with the help of a sliding median.…”

“…Structural changes in the tissue associated with the phase transitions can be assessed both in vitro and in vivo with OCT-a modern state-of-the-art noninvasive imaging modality. 9,10,35,36 OCT is a well-known optical diagnostic technique to perform real-time 2-D and/or 3-D high-resolution (∼5 to 8 μm) imaging of biological tissues in vivo with relatively high probing depth (up to 2 mm). 37,38 Principles of this imaging modality are described in detail elsewhere.…”

“…4,5 In particular, designing of optical methods for selective non-or minimally invasive diagnostics and reduction of body fat requires robust technologies to monitor condition of adipose tissue (AT). [6][7][8][9][10][11][12][13][14][15] AT is a multicomponent tissue 16 with constituents presented in Fig. 1.…”

“…14 There are not many studies on RI of AT, especially in the course of tissue heating, available in the literature. 9,[23][24][25][26][27][28][29][30][31] Therefore, in this study, the temperature dependence of RI of AT samples has been examined utilizing a combined use of optical coherence tomography (OCT) and Abbe refractometry aiming to quantify RI temperature increment and lipid phase transitions with the goal to propose an alternative method for objective indication of processes taking place during fat cell destruction/lipolysis. In general, these studies can be useful for improvement in therapeutic protocols based on thermally induced fat cell lipolysis, including laser therapy for body contouring and spot fat reduction and photochemotherapy of cancer patients.…”

In vivo detection of human cutaneous beta‐carotene using computational optical clearing

“…[23,24] Controlling the scattering properties of skin allows us to detect the chromophore hidden by absorption spectrum of blood, melanin and water. They include compression, [2,25] stretching, [26] dehydration, [27] coagulation, [28] immersion with biocompatible chemical agents, [2,6,29] photochemical [30] and photothermal [31][32][33] OC. The scattering properties of skin can be effectively changed by action of hyperosmotic immersion optical clearing agents (OCAs) (polyatomic alcohols, dimethyl sulfoxide, benzyl alcohol, saturated solutions of saccharides [carbohydrates], etc.)…”