Optical coherence tomography (Review)

Краморева, Л. И.; Rozhko, Yu. I.

doi:10.1007/s10812-010-9354-0

Cited by 10 publications

(6 citation statements)

References 74 publications

(76 reference statements)

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“…Since OCT was first described, there have been many technological innovations; however, the underlying image-forming process remains as optical scattering. For further information regarding OCT technology, the reader is referred to the extensive review literature on the subject [12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Quantitative analysis of optical coherence tomography and histopathology images of normal and dysplastic oral mucosal tissues

et al. 2011

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Selecting the most representative site for biopsy is crucial in establishing a definitive diagnosis of oral epithelial dysplasia. The current process involves clinical examination that can be subjective and prone to sampling errors. The aim of this study was therefore to investigate the use of optical coherence tomography (OCT) for differentiation of normal and dysplastic oral epithelial samples, with a view to developing an objective and reproducible approach for biopsy site selection. Biopsy samples from patients with fibro-epithelial polyps (n=13), mild dysplasia (n=2), and moderate/severe dysplasia (n=4) were scanned at 5-μm intervals using an OCT microscope and subsequently processed and stained with hematoxylin and eosin (H&E). Epithelial differentiation was measured from the rate of change (gradient) of the backscattered light intensity in the OCT signal as a function of depth. This parameter is directly related to the density of optical scattering from the cell nuclei. OCT images of normal oral epithelium showed a clear delineation of the mucosal layers observed in the matching histology. However, OCT images of oral dysplasia did not clearly identify the individual mucosal layers because of the increased density of abnormal cell nuclei, which impeded light penetration. Quantitative analysis on 2D-OCT and histology images differentiated dysplasia from normal control samples. Similar analysis on 3D-OCT datasets resulted in the reclassification of biopsy samples into the normal/mild and moderate/severe groups. Quantitative differentiation of normal and dysplastic lesions using OCT offers a non-invasive objective approach for localizing the most representative site to biopsy, particularly in oral lesions with similar clinical features.

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Section: Introductionmentioning

confidence: 99%

Quantitative analysis of optical coherence tomography and histopathology images of normal and dysplastic oral mucosal tissues

et al. 2011

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“…With quasi-monochromatic sources, the fringes are therefore visible through large path differences. However, where a broadband source illuminates the interferometer, fringes tend to wash out beyond the zero path difference (ZPD) point or the coherence gate of the light source [7,8]. This is the key feature that separates OCT sources from line sources such as Mercury lamps.…”

Section: Theory and Methodologymentioning

confidence: 99%

Development of a Free Space, LED Illuminated Spectral-domain Optical Coherence Tomography Setup

Suliali¹,

Baricholo²,

Neethling³

et al. 2017

ujpa

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Free-space spectral domain optical coherence tomography has been demonstrated using an 8 mW ultra-bright 850 nm light-emitting diode with a 40 nm spectral width. The system detects longitudinal reflectivity of surface and sub-surface layers of optical elements to depths of a millimetre with high fidelity. Development stages included mathematical analysis of light interference by superposition of electric field phasors of reference and sample arms of a Michelson interferometer. A method by which depth-resolved reflectivity is acquired is described. A locally assembled Czerny Turner monochromator was aligned such that the interferometer output beam is dispersed into its spectral components before image re-construction. Calibration of the 2048-pixel detecting charge-coupled device line camera was performed using a Mercury vapour lamp with 8 spectral lines spanning from the ultra-violet to yellow region of the electromagnetic spectrum. Processing of interference fringe signals from spectral domain data is described and an analysis of variations in frequency of the interference fringe signal and threshold illumination with depth into the sample presented. A test of sensitivity of the depth imaging algorithm to low-amplitude signals is also reported.

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“…OCT relies on interference and therefore requires stable and deterministic phase relations between the interfering waves (Kramoreva & Rozhko, 2010). Due to scattering and absorption in the examined object, the number of photons in the backscattered wave conserving stable phase relations to the photons in the incident wave reduces with depth.…”

Section: Penetration Depthmentioning

confidence: 99%