Optical detection of asymmetric bacteria utilizing electro orientation

Choi, Jae-Woo; Pu, Allen

doi:10.1364/oe.14.009780

Cited by 26 publications

(13 citation statements)

References 11 publications

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“…31 We utilize the same parameters as have been shown in the electro-orientation techniques of E. coli K12 described in previous work to align the bacteria to the electric field. 17 10 V pp at 10 MHz is applied across two electrodes to obtain a preferred alignment. Live bacteria is flowed horizontally from right to left within the microfluidic channel at a rate of 20 mm s À1 .…”

Section: Experiments 41 Electro-orientation Within a Fluidic Channelmentioning

confidence: 99%

“…[11][12][13][14][15][16] Recent studies of electro-orientation have revealed its ability to orient bacteria and nanowires. [17][18][19] Dielectrophoresis provides for concentration and sorting capabilities 11,12,16 and electro-orientation allows for the alignment of asymmetric particles on a macro scale. 20 Several methods for integration of electrodes with microfluidic channels have been developed.…”

Section: Introductionmentioning

confidence: 99%

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3-dimensional electrode patterning within a microfluidic channel using metal ion implantation

et al. 2010

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The application of electrical fields within a microfluidic channel enables many forms of manipulation necessary for lab-on-a-chip devices. Patterning electrodes inside the microfluidic channel generally requires multi-step optical lithography. Here, we utilize an ion-implantation process to pattern 3D electrodes within a fluidic channel made of polydimethylsiloxane (PDMS). Electrode structuring within the channel is achieved by ion implantation at a 40 angle with a metal shadow mask. The advantages of three-dimensional structuring of electrodes within a fluidic channel over traditional planar electrode designs are discussed. Two possible applications are presented: asymmetric particles can be aligned in any of the three axial dimensions with electro-orientation; colloidal focusing and concentration within a fluidic channel can be achieved through dielectrophoresis. Demonstrations are shown with E. coli, a rod shaped bacteria, and indicate the potential that ion-implanted microfluidic channels have for manipulations in the context of lab-on-a-chip devices.

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Section: Experiments 41 Electro-orientation Within a Fluidic Channelmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

3-dimensional electrode patterning within a microfluidic channel using metal ion implantation

et al. 2010

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“…Electro-orientation was applied to the study of several types of cells such as red blood cells 12,14 and bacteria. 15 Recently, it was reported that electro-orientation occurred during the OET manipulations of nanowires 9 and red blood cells. 11 Figures 3͑a͒-3͑d͒ show a typical alignment behavior of swimming cells in the grayscale OET.…”

Section: Programmable Manipulation Of Motile Cells In Optoelectronic mentioning

confidence: 99%

Programmable manipulation of motile cells in optoelectronic tweezers using a grayscale image

Nam

Hwang

Park

et al. 2008

Applied Physics Letters

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This paper describes a grayscale optoelectronic tweezers (OET) which allows adjustment of the electric field strength at each position of OET. A grayscale light image was used to pattern vertical electric field strength on an OET. As an electric field depends on the brightness at each point, the brighter light patterns generate the stronger electric field in the OET. Its feasibility for application to cell manipulation was demonstrated by aligning highly motile protozoan cells in vertical direction. Depending on the brightness of each pixel, the behaviors of aligned cells varied due to the different electric field strength to each cell.

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“…Various mechanisms for the reconfigurability have been realized with the application of external forces. Electrical forces have been utilized to tune a liquid lens, align birefringent material and attract or repel electronic ink [2][3][4]. Mechanical forces have been utilized to stretch and compress liquid dye laser gratings and to pneumatically pump liquid [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Tuning parameters of metal ion implantation within a microfluidic channel

et al. 2010

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Applying electrical fields is a simple and versatile method to manipulate and reconfigure optofluidic devices. Several methods to apply electric fields using electrodes on polymers or in the context of lab-on-a-chip devices exist. In this paper, we utilize an ion-implanted process to pattern electrodes within a fluidic channel made of polydimethylsiloxane (PDMS). Electrode structuring within the channel is achieved by ion implantation at a 40° angle with a metal shadow mask. In previous work using the ion-implantation process, we demonstrated two possible applications in the context of lab-on-a-chip applications. Asymmetric particles were aligned through electro-orientation. Colloidal focusing and concentration was possible with negative dielectrophoresis. In this paper, we discuss the different electrode structures that are possible by changing the channel dimensions. A second parameter of ion implantation dosage prevents the shorting of electrodes on the side wall or top wall of the fluidic channel to the bottom. This allows for floating electrodes on the side wall or top wall. These type of electrodes help prevent electrolysis as the liquid is not in direct contact with the voltage source. Possible applications of the different electrode structures that are possible are discussed.

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Optical detection of asymmetric bacteria utilizing electro orientation

Abstract: Abstract:We propose a bacterial detection scheme which uses no biochemical markers and can be applied in a Point-of-Care setting. The detection scheme aligns asymmetric bacteria with an electric field and detects the optical scattering.

Cited by 26 publications

References 11 publications

3-dimensional electrode patterning within a microfluidic channel using metal ion implantation

3-dimensional electrode patterning within a microfluidic channel using metal ion implantation

Programmable manipulation of motile cells in optoelectronic tweezers using a grayscale image

Tuning parameters of metal ion implantation within a microfluidic channel

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