Programmable manipulation of motile cells in optoelectronic tweezers using a grayscale image

Nam, Soonkeon; Hwang, Hyundoo; Park, Sungsu; Park, Je‐Kyun

doi:10.1063/1.2996277

Cited by 50 publications

(43 citation statements)

References 16 publications

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“…[11][12][13][14][15][16] Recent studies of electro-orientation have revealed its ability to orient bacteria and nanowires. [17][18][19] Dielectrophoresis provides for concentration and sorting capabilities 11,12,16 and electro-orientation allows for the alignment of asymmetric particles on a macro scale. 20 Several methods for integration of electrodes with microfluidic channels have been developed.…”

Section: Introductionmentioning

confidence: 99%

3-dimensional electrode patterning within a microfluidic channel using metal ion implantation

et al. 2010

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The application of electrical fields within a microfluidic channel enables many forms of manipulation necessary for lab-on-a-chip devices. Patterning electrodes inside the microfluidic channel generally requires multi-step optical lithography. Here, we utilize an ion-implantation process to pattern 3D electrodes within a fluidic channel made of polydimethylsiloxane (PDMS). Electrode structuring within the channel is achieved by ion implantation at a 40 angle with a metal shadow mask. The advantages of three-dimensional structuring of electrodes within a fluidic channel over traditional planar electrode designs are discussed. Two possible applications are presented: asymmetric particles can be aligned in any of the three axial dimensions with electro-orientation; colloidal focusing and concentration within a fluidic channel can be achieved through dielectrophoresis. Demonstrations are shown with E. coli, a rod shaped bacteria, and indicate the potential that ion-implanted microfluidic channels have for manipulations in the context of lab-on-a-chip devices.

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Section: Introductionmentioning

confidence: 99%

3-dimensional electrode patterning within a microfluidic channel using metal ion implantation

et al. 2010

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“…On the other hand, the optically induced torque in OEK chips has been exploited to adjust highly motile protozoan cells' moving direction [97] and induce cell selfrotation [93]. The latter method (i.e., cell self-rotation in an OEK chip) has the potential to be used to identify cell types and elucidate the electrical and physical properties of cells.…”

Section: Biological Entities: Cells and Moleculesmentioning

confidence: 99%

Micro and Nano Manipulation and Assembly by Optically Induced Electrokinetics

Lai

et al. 2015

Micro‐ and Nanomanipulation Tools

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IntroductionIn the past 25 years, integrated circuit (IC) and microelectromechanical systems (MEMSs) technologies have moved much beyond the microdomain, and into the submicro-, nanoscopic, and, even, the molecular scales. Today, we have the capability to fabricate, manipulate, and assemble matters at the micro-, nano-, or molecular scales. This progress into "seeing" and manipulating matters that are smaller than visible light wavelength scale is impacting a range of fields including semiconductor physics, biological studies, pharmaceutical development, and many other scientific and technological applications. A range of new methods to generate physical forces have been developed in the process. For example, mechanical forces can now be applied to micro-, nano-, and biological entities using atomic force microscope probes [1], microgrippers [2], or pipettes [3]. However, disadvantages of these approaches, including inflection of mechanical damages on objects being manipulated and their relatively low manipulation speed, make manipulation of large quantities of objects difficult. In order to address these disadvantages, noncontact or noninvasive methods for micro-, nano-, and biological manipulation have also been explored in the past two decades.Manipulation devices based on magnetic forces [4], acoustic waves [5,6], hydrodynamic flows [7], light waves [8], or electrokinetic forces [9] are among the major noninvasive technologies available today. Each of them has its own advantages and disadvantages. Methods based on magnetic fields need premodification on objects with magnetic beads which constrains their application domain. In addition, movement controlled by permanent magnets is not precise enough for many proposed applications. Also, the high resistance of the magnetic coil generates significant heat during manipulation [10]. Acoustic traps show good selectivity but cannot achieve parallel manipulation of single entities. Microfluidic devices exploiting hydrodynamic flows are suitable for cell population sorting, but have difficulties in trapping or controlling specific single objects.Electrokinetic forces generated from patterned electrodes capable of inducing the desired spatial distribution of electric field have been exploited to control

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“…Thus, collecting enough data in a limited amount of time requires improvement in the efficiency of observing microorganism motions along a specific direction. Z-directional (perpendicular to the top and bottom surfaces of the microfluidic channel) electromanipulation is expected to improve the efficiency of front-view observation of continuously swimming microorganisms 17,19,32,46 . To this end, electrodes with a square outline (side length of outer square ≈1000 μm, side length of inner square (observation window) ≈500 μm, thus electrode width ≈250 μm), shown in Figure 4a, were prepared on the top and bottom of the interior walls of a microchannel (~1100 μm wide, 500 μm high) using hybrid fs laser microfabrication.…”

Section: Z-directional Electro-orientation Of Microorganisms In Glassmentioning

confidence: 99%

“…The optimum frequencies for the orientation depend on the electrical and geometrical parameters of the cells and the conductivity of the cell suspensions. The electro-orientation of not only many elongated biological samples, such as E. coli (bacteria) 17,18 , B. subtilis (bacteria) 18 , S. marcescens (bacteria) 9 , T. pyriformis (ciliates) 19 , microtubules 20 , cardiac myocytes 21 , retinal photoreceptors 22 , ellipsoidal erythrocytes 23 , and yeast cells 24,25 , but also one-dimensional (1D) nanomaterials, such as nanowires and nanorods [26][27][28] , have been demonstrated. Electro-orientation in microfluidic chips has already been demonstrated for some important applications, such as the optical detection of bacteria 17 , the dielectric measurement of microtubules 20 , and cardiac tissue engineering 21 .…”

Section: Introductionmentioning

confidence: 99%

Controllable alignment of elongated microorganisms in 3D microspace using electrofluidic devices manufactured by hybrid femtosecond laser microfabrication

Kawano

Liu

et al. 2017

Microsyst Nanoeng

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This paper presents a simple technique to fabricate new electrofluidic devices for the three-dimensional (3D) manipulation of microorganisms by hybrid subtractive and additive femtosecond (fs) laser microfabrication (fs laser-assisted wet etching of glass followed by water-assisted fs laser modification combined with electroless metal plating). The technique enables the formation of patterned metal electrodes in arbitrary regions in closed glass microfluidic channels, which can spatially and temporally control the direction of electric fields in 3D microfluidic environments. The fabricated electrofluidic devices were applied to nanoaquariums to demonstrate the 3D electro-orientation of Euglena gracilis (an elongated unicellular microorganism) in microfluidics with high controllability and reliability. In particular, swimming Euglena cells can be oriented along the z-direction (perpendicular to the device surface) using electrodes with square outlines formed at the top and bottom of the channel, which is quite useful for observing the motions of cells parallel to their swimming directions. Specifically, z-directional electric field control ensured efficient observation of manipulated cells on the front side (45 cells were captured in a minute in an imaging area of~160 × 120 μm), resulting in a reduction of the average time required to capture the images of five Euglena cells swimming continuously along the z-direction by a factor of~43 compared with the case of no electric field. In addition, the combination of the electrofluidic devices and dynamic imaging enabled observation of the flagella of Euglena cells, revealing that the swimming direction of each Euglena cell under the electric field application was determined by the initial body angle.

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Programmable manipulation of motile cells in optoelectronic tweezers using a grayscale image

Cited by 50 publications

References 16 publications

3-dimensional electrode patterning within a microfluidic channel using metal ion implantation

3-dimensional electrode patterning within a microfluidic channel using metal ion implantation

Micro and Nano Manipulation and Assembly by Optically Induced Electrokinetics

Controllable alignment of elongated microorganisms in 3D microspace using electrofluidic devices manufactured by hybrid femtosecond laser microfabrication

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