Optical Fiber Sensors for Label-Free DNA Detection

Barozzi, Matteo; Manicardi, Alex; Vannucci, Armando; Candiani, Alessandro; Sozzi, Michele; Konstantaki, Maria; Pissadakis, Stavros; Corradini, Roberto; Selleri, Stefano; Cucinotta, Annamaria

doi:10.1109/jlt.2016.2607024

Cited by 49 publications

(30 citation statements)

References 60 publications

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“…The functionalization of the fiber surface leads to the binding of biological species to the glass surface that is then proved through the measurement of the fluorescence signal created by the labeled sample [37]. Several groups reported the feasibility of MOF-based biosensor for DNA detection [55,56]. Ngyuen L.V.…”

Section: Biosensors For the Selective Detection Of Specific Moleculesmentioning

confidence: 99%

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Functionalized Microstructured Optical Fibers: Materials, Methods, Applications

et al. 2020

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Microstructured optical fiber-based sensors (MOF) have been widely developed finding numerous applications in various fields of photonics, biotechnology, and medicine. High sensitivity to the refractive index variation, arising from the strong interaction between a guided mode and an analyte in the test, makes MOF-based sensors ideal candidates for chemical and biochemical analysis of solutions with small volume and low concentration. Here, we review the modern techniques used for the modification of the fiber’s structure, which leads to an enhanced detection sensitivity, as well as the surface functionalization processes used for selective adsorption of target molecules. Novel functionalized MOF-based devices possessing these unique properties, emphasize the potential applications for fiber optics in the field of modern biophotonics, such as remote sensing, thermography, refractometric measurements of biological liquids, detection of cancer proteins, and concentration analysis. In this work, we discuss the approaches used for the functionalization of MOFs, with a focus on potential applications of the produced structures.

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Section: Biosensors For the Selective Detection Of Specific Moleculesmentioning

confidence: 99%

“…The authors showed that the created sensor allows for the detection of DNA solutions at a concentration of 4 µM with the potential for further improvement. Several groups reported the feasibility of MOF-based biosensor for DNA detection [55,56]. Ngyuen L.V.…”

Section: Biosensors For the Selective Detection Of Specific Moleculesmentioning

confidence: 99%

Functionalized Microstructured Optical Fibers: Materials, Methods, Applications

et al. 2020

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“…To date, many biosensors exist which feature different detection methods including optical techniques such as optical fibres to detect DNA or bacteria [11,12]. However, the drawbacks of optically based techniques are their cost-effectiveness, complexity and relative difficulty to implement into a PoC test.…”

Section: Introductionmentioning

confidence: 99%

Establishing a Field-Effect Transistor Sensor for the Detection of Mutations in the Tumour Protein 53 Gene (TP53)—An Electrochemical Optimisation Approach

et al. 2019

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We present a low-cost, sensitive and specific DNA field-effect transistor sensor for the rapid detection of a common mutation to the tumour protein 53 gene (TP53). The sensor consists of a commercially available, low-cost, field-effect transistor attached in series to a gold electrode sensing pad for DNA hybridisation. The sensor has been predominantly optimised electrochemically, particularly with respect to open-circuit potentiometry as a route towards understanding potential (voltage) changes upon DNA hybridisation using a transistor. The developed sensor responds sensitively to TP53 mutant DNA as low as 100 nM concentration. The sensor responds linearly as a function of DNA target concentration and is able to differentiate between complementary and noncomplementary DNA target sequences.

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“…Usually, they exploit the molecular interactions between a surface and the DNA target sequence resulting in a generation of a biological layer. By monitoring the variations of the fiber transmission properties due to the bio-layer it is possible to detect the presence and concentration of particular DNA sequences [1]. In a standard optical fiber, the bio-layer can only be deposited on the outer surface of the cladding.…”

Section: Introductionmentioning

confidence: 99%

“…This reduces sensitivity, requires either strong fiber tapering [2] or tilted period Bragg gratings, and the removal of polymeric coating making the bare fiber rigid and fragile [3]. Photonic Crystal Fibers (PCFs) represent a promising platform for overcoming limits of standard optical fibers and for developing cost-effective and sensitive sensors for the detection of specific DNA sequences [1]. The air holes running along the fiber length allow the infiltration of biologically active substances close to the core.…”

Section: Introductionmentioning

confidence: 99%

Inhibited coupling guiding hollow fibers for label-free DNA detection

Giovanardi¹,

Cucinotta²,

Vincetti³

2017

Opt. Express

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The potentialities in using hollow core tube lattice fibers based on inhibited coupling wave-guiding for label-free DNA detection are numerically investigated and discussed here. The proposed sensing approach does not require any additional transducer component such as Bragg gratings, amplifying techniques such as nanoparticles nor coherent sources. It simply consists of the measurement of the transmittance of a piece of fiber some ten centimeters long. In case of matching DNA sequence, an additional bio-layer is laid down the dielectric-air interface causing a red shift of the transmission spectrum of the fiber. Results show a spectral sensitivity on the bio-layer with shift as high as 42 nm for every 10 nm of bio-layer and robustness against imperfect fiber coupling. The proposed approach can be easily applied to sensing of other complex molecular structures where the presence/absence of analyte can generate or not an additional layer.

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Optical Fiber Sensors for Label-Free DNA Detection

Cited by 49 publications

References 60 publications

Functionalized Microstructured Optical Fibers: Materials, Methods, Applications

Functionalized Microstructured Optical Fibers: Materials, Methods, Applications

Establishing a Field-Effect Transistor Sensor for the Detection of Mutations in the Tumour Protein 53 Gene (TP53)—An Electrochemical Optimisation Approach

Inhibited coupling guiding hollow fibers for label-free DNA detection

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