Optical high-resolution analysis of rotational movement: testing circular spatial filter velocimetry (CSFV) with rotating biological cells

Schaeper, Martin; Schmidt, Robin; Kostbade, R.; Damaschke, Nils; Gimsa, Jan

doi:10.1088/0022-3727/49/26/265402

Cited by 6 publications

(5 citation statements)

References 41 publications

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“…The image quality would need to be further improved in order to acquire spectra with such low magnification. Filters could help improving the contrast . Another possibility could be to use an automatized microscope stage to observe all 39 quadrupoles with a 20X magnification in sequential videos.…”

Section: Resultsmentioning

confidence: 99%

On‐chip technology for single‐cell arraying, electrorotation‐based analysis and selective release

et al. 2019

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This paper reports a method for label‐free single‐cell biophysical analysis of multiple cells trapped in suspension by electrokinetic forces. Tri‐dimensional pillar electrodes arranged along the width of a microfluidic chamber define actuators for single cell trapping and selective release by electrokinetic force. Moreover, a rotation can be induced on the cell in combination with a negative DEP force to retain the cell against the flow. The measurement of the rotation speed of the cell as a function of the electric field frequency define an electrorotation spectrum that allows to study the dielectric properties of the cell. The system presented here shows for the first time the simultaneous electrorotation analysis of multiple single cells in separate micro cages that can be selectively addressed to trap and/or release the cells. Chips with 39 micro‐actuators of different interelectrode distance were fabricated to study cells with different sizes. The extracted dielectric properties of Henrietta Lacks, human embryonic kidney 293, and human immortalized T lymphocytes cells were found in agreements with previous findings. Moreover, the membrane capacitance of M17 neuroblastoma cells was investigated and found to fall in in the range of 7.49 ± 0.39 mF/m2.

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Section: Resultsmentioning

confidence: 99%

On‐chip technology for single‐cell arraying, electrorotation‐based analysis and selective release

et al. 2019

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“…In summary, the search for consistent dielectric object properties will be more promising the greater the number of methods employed. There is hope that the combination of multiple AC-electrokinetic methods may lead to a new kind of DSCS fingerprinting with a higher resolution for cell and particle structures [29,34,35,65]. The author has declared no conflict of interest.…”

Section: Discussionmentioning

confidence: 99%

Combined AC‐electrokinetic effects: Theoretical considerations on a three‐axial ellipsoidal model

Gimsa

2018

Electrophoresis

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AC fields induce charges at the structural interfaces of particles or biological cells. The interaction of these charges with the field generates frequency‐dependent forces that are the basis for AC‐electrokinetic effects such as dielectrophoresis (DEP), electrorotation (ROT), electro‐orientation, and electro‐deformation. The effects can be used for the manipulation or dielectric single‐particle spectroscopy. The observation of a particular effect depends on the spatial and temporal field distributions, as well as on the shape and the dielectric and viscoelastic properties of the object. Because the effects are not mutually independent, combined frequency spectra are obtained, for example, discontinuous DEP and ROT spectra with ranges separated by the reorientation of nonspherical objects in the linearly and circularly polarized DEP and ROT fields, respectively. As an example, the AC electrokinetic behavior of a three‐axial ellipsoidal single‐shell model with the geometry of chicken‐red blood cells is considered. The geometric and electric problems were separated using the influential‐radius approach. The obtained finite‐element model can be electrically interpreted by an RC model leading to an expression for the Clausius–Mossotti factor, which permits the derivation of force, torque, and orientation spectra, as well as of equations for the critical frequencies and force plateaus in DEP and of the characteristic frequencies and peak heights in ROT. Expressions for the orientation in linearly and circularly polarized fields, as well as for the reorientation frequencies were also derived. The considerations suggested that the simultaneous registration of various AC‐electrokinetic spectra is a step towards the dielectric fingerprinting of single objects.

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“…The measuring chamber for ROT was based on a rectangular glass microchip with four round‐tip electrodes (Fig. A) . ROT spectra were recorded in the 50 kHz to 150 MHz range, as described previously , using a HP 8130A generator (Hewlett Packard, USA).…”

Section: Methodsmentioning

confidence: 99%

“…The models make it possible to recalculate electric cell properties from the spectra obtained by different AC‐electrokinetic methods. In the field of biology, interesting applications are the determination, screening or the pursuit of changes in membrane capacitance, membrane conductance, and the cytoplasmic properties of single cells . The methods are also applied to analyze the properties of colloids, nanoparticles, and viruses .…”

Section: Introductionmentioning

confidence: 99%

Combined detection of AC‐electrokinetic effects: Experiments with three‐axial chicken red blood cells

et al. 2018

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Dielectrophoresis (DEP), electrorotation (ROT), and electro-orientation were used for the dielectric spectroscopy of nucleated three-axial chicken red blood cells (CRBCs). Because the different AC-electrokinetic effects are not mutually independent, their DEP and ROT spectra were combined in ranges separated by the reorientation of the CRBCs in the inhomogeneous linear DEP and circular ROT fields. This behavior can be qualitatively described by a single-shell ellipsoidal model. Whereas in linear fields, the maximum of the Clausius-Mossotti factor along the three axes determines the orientated axis, in circular fields, the minimum of the factor determines the axis perpendicularly orientated to the field plane. Quantitatively, it has not been possible to find a consistent parameter set for fitting the DEP and ROT spectra, as well as the reorientation frequencies. Our ellipsoidal CRBC standard model had semiaxes of a = 7.7 μm, b = 4.0 μm, and c = 1.85 μm, a relative permittivity of 35 to 45 and conductivity of 0.36 to 0.04 S/m for the cytoplasm, combined with a specific capacitance of 10 to 14 mF/m and a conductivity of 3500 S/m for the cell membrane. The fits in different external conductivity ranges between external conductivities of 0.015 and 1.0 S/m were improved when the membrane capacitance was changed between 4 to 25 mF/m depending on the method used. A similar transition was reflected in the effective properties of a three-shell spherical model containing an internal membranous sphere with the geometry of the CRBC nucleus. Our findings suggest that the simultaneous interpretation of various AC-electrokinetic spectra is a step toward the dielectric fingerprinting of biological cells.

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Optical high-resolution analysis of rotational movement: testing circular spatial filter velocimetry (CSFV) with rotating biological cells

Cited by 6 publications

References 41 publications

On‐chip technology for single‐cell arraying, electrorotation‐based analysis and selective release

On‐chip technology for single‐cell arraying, electrorotation‐based analysis and selective release

Combined AC‐electrokinetic effects: Theoretical considerations on a three‐axial ellipsoidal model

Combined detection of AC‐electrokinetic effects: Experiments with three‐axial chicken red blood cells

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