Optical imaging of bacterial infection models

Leevy, W. Matthew; Serazin, Nathan; Smith, Bradley D.

doi:10.1016/j.ddmod.2007.07.001

Cited by 16 publications

(15 citation statements)

References 39 publications

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“…However, other infection sites, like the kidney, which is an avenue for probe clearance, would be more difficult to image at this lower probe concentration since there is a higher background signal due to some nonselective accumulation of probe. 1 The values of T/NT and T/L reported here compare favorably with an imaging study that utilized radiolabeled antimicrobial peptides and reported T/NT ratios as high as 4.1 ± 0.7 (30). A value for T/L could not be determined since most of the signal originated from the body of the mouse and not the target site.…”

Section: In Vivo Imaging With Lower Probe Concentrationsupporting

confidence: 76%

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Noninvasive Optical Imaging of Staphylococcus aureus Bacterial Infection in Living Mice Using a Bis-Dipicolylamine-Zinc(II) Affinity Group Conjugated to a Near-Infrared Fluorophore

et al. 2008

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Optical imaging of bacterial infection in living animals is usually conducted with genetic reporters such as light emitting enzymes or fluorescent proteins. However, there are many circumstances where genetic reporters are not applicable, and there is a need for exogenous synthetic probes that can selectively target bacteria. The focus of this study is a fluorescent imaging probe that is composed of a bacterial affinity group conjugated to a near infrared dye. The affinity group is a synthetic zinc (II) coordination complex that targets the anionic surfaces of bacterial cells. The probe allows detection of Staphylococcus aureus infection (5 × 10 7 cells) in a mouse leg infection model using whole animal near infrared fluorescence imaging. Region of interest analysis showed that the signal ratio for infected leg to uninfected leg reaches 3.9 ± 0.5 at 21 h post-injection of the probe. Ex vivo imaging of the organs produced a signal ratio of 8 for infected to uninfected leg. Immunohistochemical analysis confirmed that the probe targeted the bacterial cells in the infected tissue. Optimization of the imaging filter set lowered the background signal due to autofluorescence and substantially improved imaging contrast. The study shows that near infrared molecular probes are amenable to non-invasive optical imaging of localized S. aureus infection.

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Section: In Vivo Imaging With Lower Probe Concentrationsupporting

confidence: 76%

“…In most cases, genetic reporter systems such as light emitting luciferase enzymes, or fluorescent proteins like GFP, have been employed with some notable success (1). However, pathogenic bacteria in their native environments do not express endogenous optical reporters.…”

Section: Introductionmentioning

confidence: 99%

Noninvasive Optical Imaging of Staphylococcus aureus Bacterial Infection in Living Mice Using a Bis-Dipicolylamine-Zinc(II) Affinity Group Conjugated to a Near-Infrared Fluorophore

et al. 2008

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“…Optical molecular imaging [13], [14] can detect light emitted from chemiluminescent or fluorescent probes and is relatively safe, cheap and easy to handle compared with MRI or nuclear imaging. Fluorescent chemosensors selectively bind to ions [15] or metals [16], changing their emission or excitation/emission profiles.…”

Section: Introductionmentioning

confidence: 99%

In Vivo Fluorescence Imaging of Bacteriogenic Cyanide in the Lungs of Live Mice Infected with Cystic Fibrosis Pathogens

et al. 2011

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Background Pseudomonas aeruginosa (PA) and Burkholderia cepacia complex (Bcc), commonly found in the lungs of cystic fibrosis (CF) patients, often produce cyanide (CN), which inhibits cellular respiration. CN in sputa is a potential biomarker for lung infection by CF pathogens. However, its actual concentration in the infected lungs is unknown.Methods and FindingsThis work reports observation of CN in the lungs of mice infected with cyanogenic PA or Bcc strains using a CN fluorescent chemosensor (4′,5′-fluorescein dicarboxaldehyde) with a whole animal imaging system. When the CN chemosensor was injected into the lungs of mice intratracheally infected with either PA or B. cepacia strains embedded in agar beads, CN was detected in the millimolar range (1.8 to 4 mM) in the infected lungs. CN concentration in PA-infected lungs rapidly increased within 24 hours but gradually decreased over the following days, while CN concentration in B. cepacia-infected lungs slowly increased, reaching a maximum at 5 days. CN concentrations correlated with the bacterial loads in the lungs. In vivo efficacy of antimicrobial treatments was tested in live mice by monitoring bacteriogenic CN in the lungs.ConclusionsThe in vivo imaging method was also found suitable for minimally invasive testing the efficacy of antibiotic compounds as well as for aiding the understanding of bacterial cyanogenesis in CF lungs.

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“…22 In this paper, hydrophilic ICG derivative dye ICG-Der-02(excitation/emission 783/ 810 nm) with deeper tissue penetration and faster clearance rate 23 was chosen to conjugate with UBI29-41 peptide to form a NIR probe. We now describe a detailed optical imaging investigation of S. aureus infection from the whole animal.…”

Section: à10mentioning

confidence: 99%

NONINVASIVE OPTICAL IMAGING OF STAPHYLOCOCCUS AUREUS INFECTION IN VIVO USING AN ANTIMICROBIAL PEPTIDE FRAGMENT BASED NEAR-INFRARED FLUORESCENT PROBES

Liu

2013

J. Innov. Opt. Health Sci.

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The diagnosis of bacterial infections remains a major challenge in medicine. Optical imaging of bacterial infection in living animals is usually conducted with genetic reporters such as lightemitting enzymes or°uorescent proteins. However, there are many circumstances where genetic reporters are not applicable, and there is an urgent need for exogenous synthetic probes that can selectively target bacteria. Optical imaging of bacteria in vivo is much less developed than methods such as radioimaging and MRI. Furthermore near-infrared (NIR) dyes with emission wavelengths in the region of 650À900 nm can propagate through two or more centimeters of tissue and may enable deeper tissue imaging if sensitive detection techniques are employed. Here we constructed an antimicrobial peptide fragment UBI29-41-based near-infrared°uorescent imaging probe. The probe is composed of UBI29-41 conjugated to a near infrared dye ICG-Der-02. UBI29-41 is a cationic antimicrobial peptide that targets the anionic surfaces of bacterial cells. The probe allows detection of Staphylococcus aureus infection (5Â10 7 cells) in a mouse local infection model using whole animal near-infrared°uorescence imaging. Furthermore, we demonstrate that the UBI29-41-based imaging probe can selectively accumulate within bacteria. The signi¯cantly higher accumulation in bacterial infection suggests that UBI29-41-based imaging probe may be a promising imaging agent to detect bacterial infections.

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Optical imaging of bacterial infection models

Cited by 16 publications

References 39 publications

Noninvasive Optical Imaging of Staphylococcus aureus Bacterial Infection in Living Mice Using a Bis-Dipicolylamine-Zinc(II) Affinity Group Conjugated to a Near-Infrared Fluorophore

Noninvasive Optical Imaging of Staphylococcus aureus Bacterial Infection in Living Mice Using a Bis-Dipicolylamine-Zinc(II) Affinity Group Conjugated to a Near-Infrared Fluorophore

In Vivo Fluorescence Imaging of Bacteriogenic Cyanide in the Lungs of Live Mice Infected with Cystic Fibrosis Pathogens

NONINVASIVE OPTICAL IMAGING OF STAPHYLOCOCCUS AUREUS INFECTION IN VIVO USING AN ANTIMICROBIAL PEPTIDE FRAGMENT BASED NEAR-INFRARED FLUORESCENT PROBES

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