2014
DOI: 10.1038/nprot.2014.028
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Optical measurements to determine the thickness of calcite crystals and the mass of thin carbonate particles such as coccoliths

Abstract: We describe a procedure for measuring the thickness and mass of calcite particles that works for most calcite particles<4.5-μm thick. The calcite particles are observed in cross-polarized light, which enables the light transmitted through the calcite particles to be correlated with their thickness. Three polarizing planes are used to minimize the darkening of crystals at some orientations (black cross). This allows direct measurement of the thickness without recourse to a transfer function. This procedure has … Show more

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Cited by 92 publications
(131 citation statements)
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“…It therefore seems that within one strain, mean coccolith volume is not a plastic trait. Using a conversion factor for mean grey level (MGL) to coccolith thickness (Beaufort et al, 2014), we estimated mean coccolith calcite weight for the two subspecies. Subspecies braarudii produced coccoliths with an average weight of 117 ± 34 pg calcite whereas subsp.…”
Section: Effect Of Phosphorus Limitation and Temperature On Coccolithmentioning
confidence: 99%
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“…It therefore seems that within one strain, mean coccolith volume is not a plastic trait. Using a conversion factor for mean grey level (MGL) to coccolith thickness (Beaufort et al, 2014), we estimated mean coccolith calcite weight for the two subspecies. Subspecies braarudii produced coccoliths with an average weight of 117 ± 34 pg calcite whereas subsp.…”
Section: Effect Of Phosphorus Limitation and Temperature On Coccolithmentioning
confidence: 99%
“…Filters were dried at 60 • C in a drying oven, mounted with Canada Balsam (Merck, USA) on microscope slides and viewed under crossed polarizers with a DM6000B Leica microscope, which had a modified turret accommodating polarizing filters at different angles (Beaufort et al, 2014). Individual coccoliths were randomly selected and captured at 1000× magnification with a SPOT Flex colour digital camera (Diagnostic Instruments Inc., USA) and subsequently processed to a compound 8 bit greyscale image (Beaufort et al, 2014). A custom-made macro in ImageJ freeware measured coccolith length through an ellipse approximation, as well as mean Table 2.…”
Section: Coccosphere and Coccolith Morphology 241 Coccolith Dimensionsmentioning
confidence: 99%
“…2014) permit such calculations with a single image rather than composite at several orientations as was done in previous approaches (Beaufort et al, 2014).…”
Section: Introductionmentioning
confidence: 99%
“…This calcite is believed to be key to ocean biogeochemical cycles, as it increases deep export of organic carbon by ballasting of aggregates (Abrantes et al, 2002;Armstrong et al, 2002;Armstrong et al, 2009;Bárcena et al, 2004;Berger et al, 1989;Engel et al, 2009;Iversen and Ploug, 2010;Milliman, 1993;Ridgwell and Zeebe, 2005;Ziveri et al, 2007). 25…”
Section: Introductionmentioning
confidence: 99%
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