Optical Method for Estimating the Chlorophyll Contents in Plant Leaves

Pérez-Patricio, Madaín; Camas-Anzueto, J.L.; Sanchez-Alegría, Avisaí; Aguilar-González, Abiel; Gutiérrez-Miceli, Federico Antonio; Escobar-Gómez, Elías N.; Voisin, Yvon; Rios-Rojas, Carlos; Grajales-Coutiño, R.

doi:10.3390/s18020650

Cited by 92 publications

(50 citation statements)

References 34 publications

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“…Protein concentration was determined based on a bovine serum albumin standard curve. Total chlorophyll concentration was measured according to Pérez-Patricio et al (2018) . An aliquot of 100 mg of fresh leaf tissue was ground in 5 ml of acetone (80%, v/v).…”

Section: Determination Of Plant and Rhizosphere Parametersmentioning

confidence: 99%

Phosphate Solubilizing Rhizobacteria Could Have a Stronger Influence on Wheat Root Traits and Aboveground Physiology Than Rhizosphere P Solubilization

et al. 2020

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Limited P availability in several agricultural areas is one of the key challenges facing current agriculture. Exploiting P-solubilizing bacteria (PSB) has been an emerging bio-solution for a higher rhizosphere P-availability, meanwhile the above-and below-ground interactions that PSB would trigger remain unclear over plant growing stages. We hypothesized that PSB effects on plant growth may be greater on root traits that positively links with aboveground physiology more than the commonly believed rhizosphere P bio-solubilization. In this study, five contrasting PSB (Pseudomonas spp.) isolates (low "PSB 1 ", moderate "PSB 2 and PSB 4 " and high "PSB 3 and PSB 5 " P-solubilizing capacity "PSC") were used to investigate aboveand below-ground responses in wheat fertilized with rock P (RP) under controlled conditions. Our findings show that all PSB isolates increased wheat root traits, particularly PSB 5 which increased root biomass and PSB 3 that had greater effect on root diameter in 7-, 15-and 42day old plants. The length, surface and volume of roots significantly increased along with higher rhizosphere available P in 15-and 42-day old plants inoculated with PSB 4 and PSB 2. Shoot biomass significantly increased with both PSB 2 and PSB 5. Root and shoot physiology significantly improved with PSB 1 (lowest PSC) and PSB 4 (moderate PSC), notably shoot total P (78.38%) and root phosphatase activity (390%). Moreover, nutrients acquisition and chlorophyll content increased in inoculated plants and was stimulated (PSB 2 , PSB 4) more than rhizosphere P-solubilization, which was also revealed by the significant above-and below-ground inter-correlations, mainly chlorophyll and both total (R = 0.75, p = 0.001**) and intracellular (R = 0.7, p = 0.000114*) P contents. These findings demonstrate the necessity to timely monitor the plant-rhizosphere continuum responses, which may be a relevant approach to accurately evaluate PSB through considering below-and above-ground relationships; thus enabling unbiased interpretations prior to field applications.

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Section: Determination Of Plant and Rhizosphere Parametersmentioning

confidence: 99%

Phosphate Solubilizing Rhizobacteria Could Have a Stronger Influence on Wheat Root Traits and Aboveground Physiology Than Rhizosphere P Solubilization

et al. 2020

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“…Approximately 0.05 g of fresh leaf tissue was extracted with 95% ethanol to determine the content of photosynthetic pigments (chlorophyll a and chlorophyll b). All samples were analyzed using a spectrophotometer (UV-1280, Shimadzu, Kyoto, Japan) at wavelengths of 663 and 645 nm [76]. The whole process was accomplished in a low-radiance environment.…”

Section: Determination Of Photosynthetic Pigmentsmentioning

confidence: 99%

Nitrogen Enhances Salt Tolerance by Modulating the Antioxidant Defense System and Osmoregulation Substance Content in Gossypium hirsutum

Sikder

Wang

Zhang

et al. 2020

Plants

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Increasing soil salinity suppresses both productivity and fiber quality of cotton, thus, an appropriate management approach needs to be developed to lessen the detrimental effect of salinity stress. This study assessed two cotton genotypes with different salt sensitivities to investigate the possible role of nitrogen supplementation at the seedling stage. Salt stress induced by sodium chloride (NaCl, 200 mmol·L −1 ) decreased the growth traits and dry mass production of both genotypes. Nitrogen supplementation increased the plant water status, photosynthetic pigment synthesis, and gas exchange attributes. Addition of nitrogen to the saline media significantly decreased the generation of lethal oxidative stress biomarkers such as hydrogen peroxide, lipid peroxidation, and electrolyte leakage ratio. The activity of the antioxidant defense system was upregulated in both saline and non-saline growth media as a result of nitrogen application. Furthermore, nitrogen supplementation enhanced the accumulation of osmolytes, such as soluble sugars, soluble proteins, and free amino acids. This established the beneficial role of nitrogen by retaining additional osmolality to uphold the relative water content and protect the photosynthetic apparatus, particularly in the salt-sensitive genotype. In summary, nitrogen application may represent a potential strategy to overcome the salinity-mediated impairment of cotton to some extent.Plants 2020, 9, 450 2 of 20 most sensitivity to salinity during the seedling growth stage, and remained sensitive at the reproductive stage [9]. Excess salinity inhibits plant growth and development by inducing osmotic stress, particular ion toxicity (Na + and Cl − ), oxidative damage, and/or nutritional disorders in plant tissues, which subsequently lead to weakened plant growth and endurance [10][11][12]. Salt stress has been shown to significantly decrease the uptake and metabolism of numerous mineral nutrients (such as nitrogen, potassium, phosphorus, and calcium), and accelerate the damage of the photosynthetic machinery as well as the whole salt tolerance mechanisms of plants. Salt stress-induced plant metabolism alterations are the secondary consequence of carbon and nitrogen metabolism [13]. Moreover, a high salt concentration perturbs electron transport systems in both chloroplasts and mitochondria [14,15]. This accelerates electron leakage from electron transportation chains and induces the generation of reactive oxygen species (ROS), such as superoxide radicals, hydroxyl radicals, and hydrogen peroxide [16,17]. The over-accumulation of ROS not only damages cellular and biological activities [18][19][20], but also degrades chlorophyll pigments and obstructs the synthesis of proteins, amino acids, lipids, deoxyribonucleic acid, as well as enzymatic and non-enzymatic activities of plants [17]. To avoid ROS-induced oxidative damage, plant-assured endogenous tolerance approaches, such as the antioxidant defense system as well as the accumulation of organic solutes and secondary metabolites [21]....

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“…1b). SPAD and CHL indices are similar because each instrument indirectly estimates the chlorophyll content in the leaf (Padilla et al 2018;Parry et al 2014;Perez-Patricio et al 2018), and there is a positive relationship between SPAD and the CHL index (Padilla et al 2018).…”

Section: Yield Prognostic Indexmentioning

confidence: 99%

Application of Proximal Optical Sensors to Assess Nitrogen Status and Yield of Bell Pepper Grown in Slab

Silva

Fontes

Milagres

et al. 2020

J Soil Sci Plant Nutr

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Nitrogen (N) is an essential element frequently associated with environmental issues, and the utilization of leaf clip sensors to adjust N fertilization is a technology of interest. This study aimed to evaluate the indices measured with SPAD and Dualex through the bell pepper crop cycle cultivated under different N doses, to identify the ability of the SPAD and Dualex optical sensors to assess N status and establishing the accuracy of the chlorophyll indices as yield estimators. Bell pepper plants were cultivated for 150 days after transplanting (DAT) in slabs fertigated with 6 different N doses divided into 10 biweekly applications. The indices SPAD, chlorophyll (CHL), flavonoids (FLV), and nitrogen balance index (NBI) were measured over the cycle on the uppermost mature leaves of each treatment. We determined the N content of these leaves and the final commercial yield. An interaction effect was found between N dose and growth stage. The SPAD and CHL indices were not significantly affected by N dose up to 60 DAT. The FLV and NBI indices show an opposite pattern in response to N dose. We found a strong correlation between the SPAD, CHL, and NBI and the N content in the leaves and between these indices and commercial yield in several evaluated dates. However, we did not observe a significant correlation between commercial yield and FLV. Both leaf clip sensors could predict the N status of the leaves and the commercial yield, improving the correlation as the crop development progressed.

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Optical Method for Estimating the Chlorophyll Contents in Plant Leaves

Cited by 92 publications

References 34 publications

Phosphate Solubilizing Rhizobacteria Could Have a Stronger Influence on Wheat Root Traits and Aboveground Physiology Than Rhizosphere P Solubilization

Phosphate Solubilizing Rhizobacteria Could Have a Stronger Influence on Wheat Root Traits and Aboveground Physiology Than Rhizosphere P Solubilization

Nitrogen Enhances Salt Tolerance by Modulating the Antioxidant Defense System and Osmoregulation Substance Content in Gossypium hirsutum

Application of Proximal Optical Sensors to Assess Nitrogen Status and Yield of Bell Pepper Grown in Slab

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