Optical method for the determination of the oxygen‐transfer capacity of small bioreactors based on sulfite oxidation

Hermann, Róbert; Walther, Nico; Maier, Ulrike; Büchs, Jochen

doi:10.1002/bit.1126

Cited by 88 publications

(109 citation statements)

References 28 publications

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“…For the 24-well plate system, k L a's were determined by the sulfite oxidation method as adapted for microtiter plates by Hermann et al (2001Hermann et al ( , 2002. The reactors were filled with 1 mL of a solution of 0.5 M sodium sulfite, 0.012 M sodium phosphate, <1 mM cobalt sulfate, and the pH indicator dye HPTS, adjusted to pH 8.…”

Section: -Well Plate Systemmentioning

confidence: 99%

Design and performance of a 24‐station high throughput microbioreactor

Harms

Kostov

French

et al. 2005

Biotech & Bioengineering

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Two prototype 24-unit microbioreactors are presented and reviewed for their relative merits. The first used a standard 24-well plate as the template, while the second consisted of 24-discrete units. Both systems used non-invasive optical sensors to monitor pH and dissolved oxygen. The systems were used to cultivate Escherichia coli. Both designs had their merits and the results obtained are presented. In addition, dissolved oxygen control was demonstrated at the milliliter scale and 24 simultaneously monitored fermentations were successfully carried out. These results demonstrated high quality high throughput bioprocessing and provide important insights into operational parameters at small scale. ß 2005 Wiley Periodicals, Inc.

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Section: -Well Plate Systemmentioning

confidence: 99%

Design and performance of a 24‐station high throughput microbioreactor

Harms

Kostov

French

et al. 2005

Biotech & Bioengineering

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“…Two types of specially designed orbital shakers were used: 1) A modified table shaker (ES-W, Kühner, Basel, Switzerland) allowing different shaking diameters and shaking frequencies of up to 1,000 L/min (Hermann et al, 2001(Hermann et al, , 2003, was used for experiments with a shaking diameter of 3, 6, 12.5, 25, and 50 mm. 2) Three separate, new, custommade variants of an MTP shaker (Teleshake, H+P Labortechnik, Oberschleissheim, Germany) were used.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, a new optical method has been developed for the characterization of small-scale reactors where no DOelectrodes or exhaust gas analysis can be applied (Hermann et al, 2001). The sulfite becomes oxidized to the more acidic sulfate, which leads to a drop of the pH at the end of the reaction.…”

Section: Theory Mass Transfer With Chemical Reactionmentioning

confidence: 99%

Oxygen transfer phenomena in 48‐well microtiter plates: Determination by optical monitoring of sulfite oxidation and verification by real‐time measurement during microbial growth

Kensy

Zimmermann²,

Knabben³

et al. 2005

Biotech & Bioengineering

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Oxygen limitation is one of the most frequent problems associated with the application of shaking bioreactors. The gas-liquid oxygen transfer properties of shaken 48-well microtiter plates (MTPs) were analyzed at different filling volumes, shaking diameters, and shaking frequencies. On the one hand, an optical method based on sulfite oxidation was used as a chemical model system to determine the maximum oxygen transfer capacity (OTR(max)). On the other hand, the Respiration Activity Monitoring System (RAMOS) was applied for online measurement of the oxygen transfer rate (OTR) during growth of the methylotropic yeast Hansenula polymorpha. A proportionality constant between the OTR(max) of the biological system and the OTR(max) of the chemical system were indicated from these data, offering the possibility to transform the whole set of chemical data to biologically relevant conditions. The results exposed "out of phase" shaking conditions at a shaking diameter of 1 mm, which were confirmed by theoretical consideration with the phase number (Ph). At larger shaking diameters (2-50 mm) the oxygen transfer rate in MTPs shaken at high frequencies reached values of up to 0.28 mol/L/h, corresponding to a volumetric mass transfer coefficient (k(L)a) of 1,600 1/h. The specific mass transfer area (a) increases exponentially with the shaking frequency up to values of 2,400 1/m. On the contrary, the mass transfer coefficient (k(L)) is constant at a level of about 0.15 m/h over a wide range of shaking frequencies and shaking diameters. However, at high shaking frequencies, when the complete liquid volume forms a thin film on the cylindric wall of the well, the mass transfer coefficient (k(L)) increases linearly to values of up to 0.76 m/h. Essentially, the present investigation demonstrates that the 48-well plate outperforms the 96-well MTP and shake flasks at widely used operating conditions with respect to oxygen supply. The 48-well plates emerge, therefore, as an excellent alternative for microbial cultivation and expression studies combining the advantages of both the high-throughput 96-well MTP and the classical shaken Erlenmeyer flask.

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“…Several works are found in literature about the role of oxygen scavengers emulating bacterial respiration [7], [8] [9], [10]. However, most of these contributions are focused on catalyzed reactions.…”

Section: Oxygen Scavengermentioning

confidence: 99%

Comparison of empirical correlations for the estimation of the oxygen transfer coefficient in an aerobic bioprocess

et al. 2016

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Abstract. This paper shows a comparison of three different empirical correlations found in the literature for the estimation of the oxygen transfer coefficient in an aeration pilot plant using a phenomenological-based semi-physical model (PBSM). The relationship between each empirical correlation and the oxygen transfer phenomenon from the gas phase to the liquid phase was assessed. The results showed that empirical correlations of the oxygen transfer coefficient found in the literature are not based on the knowledge of the physical phenomena, and hence are not suitable to generalize the transference mechanism in similar processes. Keywords

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Optical method for the determination of the oxygen‐transfer capacity of small bioreactors based on sulfite oxidation

Cited by 88 publications

References 28 publications

Design and performance of a 24‐station high throughput microbioreactor

Design and performance of a 24‐station high throughput microbioreactor

Oxygen transfer phenomena in 48‐well microtiter plates: Determination by optical monitoring of sulfite oxidation and verification by real‐time measurement during microbial growth

Comparison of empirical correlations for the estimation of the oxygen transfer coefficient in an aerobic bioprocess

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