Optical methods of studying the effect of laser radiation on human erythrocytes

Korolevich, Alexander N.; Oleinik, T. V.; Khairullina, A. Ya.

doi:10.1007/bf00667665

Cited by 7 publications

(2 citation statements)

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“…The solution of this problem will aid in the further development of practical medicine. Despite the fact that the response of blood to the action of a low-intensity laser radiation gives important information on the mechanism of interaction of laser radiation with a living organism [8,9], only a small number of works have been devoted to such investigations in living organisms. In the present work, we carried out spectral investigations of the response of the blood of experimental animals (rabbits) to the intravenous irradiation by laser radiation.…”

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confidence: 99%

Interaction of Low-Intensity Laser Radiation with Blood and Its Components

Zalesskaya

Sambor

2005

J Appl Spectrosc

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The response of the blood of rabbits to the intravenous irradiation by a He-Ne laser radiation (λ = 632.8 nm) has been investigated by the UV-visible and IR absorption spectra of the whole blood, plasma, and erythrocyte mass. It has been established that hemoglobin is a primary photoacceptor absorbing low-intensity HeNe-laser radiation. The exposure of blood to this radiation causes clearly defined changes in the IR and visible absorption spectra of the blood and erythrocytes. These spectral changes arise as a result of partial photodissociation of hemoglobin-ligand complexes in the process of absorption of laser radiation. It is suggested that photodissociation is a primary reaction that arises in blood exposed to a low-intensity laser radiation.Keywords: low-intensity laser radiation, intravenous irradiation of blood, hemoglobin, UV-visible and IR spectra of blood, hemoglobin photodissociation.Introduction. At present, low-intensity laser radiation is widely used in clinical practice. The intravenous irradiation of blood is considered as the most efficient method of biostimulation of an organism for therapeutic purposes. The effects, including the therapeutic effect, arising in living organisms exposed to laser radiation for the purpose of treatment of various diseases have been described in detail in the literature [1,2]. The ability of living biosystems to transform their metabolism under the action of low-intensity laser radiation was revealed for the first time from the spectral dependences of the photosensitivity of various bioprocesses to laser radiation [3][4][5]. However, up to now there has been no unique theory developed to explain the mechanism of action of a low-intensity laser radiation on even the simplest bioobjects, such as cells and biocultures, and the effect arising in this case [4,6,7]. Therefore, the study of the mechanism of interaction of a low-intensity laser radiation with living organisms by various methods for the purpose of widening the field of its medical application is undoubtedly a pressing problems. The solution of this problem will aid in the further development of practical medicine. Despite the fact that the response of blood to the action of a low-intensity laser radiation gives important information on the mechanism of interaction of laser radiation with a living organism [8,9], only a small number of works have been devoted to such investigations in living organisms. In the present work, we carried out spectral investigations of the response of the blood of experimental animals (rabbits) to the intravenous irradiation by laser radiation. The changes in the spectral characteristics of the blood exposed to a low-intensity laser radiation were analyzed using the IR (range 4-15 µm) and UV-visible (range 200-800 nm) absorption spectra of the whole blood, plasma, and erythrocyte mass.Experimental Procedure. Samples of the whole venous blood of experimental animals (rabbits) taken before and after the intravenous irradiation of them by a low-intensity He-Ne laser (HNL) radiatio...

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confidence: 99%

Interaction of Low-Intensity Laser Radiation with Blood and Its Components

Zalesskaya

Sambor

2005

J Appl Spectrosc

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“…Blood properties, of patients with different pathologies, are modified with LLL irradiation. 11 Laser irradiation improves microcirculation, and modulates rheological properties of blood pathology. 12 Laser irradiation induces conformational transitions of the red blood cell membrane, which is related to changes in the structural states of both erythrocyte membrane proteins and lipid bilayer, resulting in changes in the activity of membrane ion pumps.…”

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In VitroMean Red Blood Cell Volume Change Induced by Diode Pump Solid State Low-Level Laser of 405 nm

Musawi

Jafar

Al-Gailani

et al. 2016

Photomedicine and Laser Surgery

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Objective: This study was conducted to investigate the effects of low-level laser (LLL) doses on human red blood cell volume. The effects of exposure to a diode pump solid state (DPSS) (λ = 405 nm) laser were observed. Background data: The response of human blood to LLL irradiation gives important information about the mechanism of interaction of laser light with living organisms. Materials and methods Blood samples were collected into ethylenediaminetetraacetic acid (EDTA)-containing tubes, and each sample was divided into two equal aliquots, one to serve as control and the other for irradiation. The aliquot was subjected to laser irradiation for 20, 30, 40, or 50 min at a fixed power density of 0.03 W/cm2. Mean cell volume (MCV) and red blood cell (RBC) counts were measured immediately after irradiation using a computerized hemtoanalyzer. Results: Significant decrease in RBC volume (p < 0.05, p < 0.0001, p < 0.0001, and p < 0.05, respectively) was induced with variation in laser doses.The highest response was observed with an exposure time of 40 min. This result was reproduced in RBCs suspended in a buffered NaCl solution. In contrast to this finding, laser-induced RBC volume change was completely abolished by suspending RBCs in a solution containing a higher concentration of EDTA. Conclusions: It was suggested that LLL can reduce RBC volume possibly because of the increased free intracellular Ca+2 concentrations, which activate Ca+2-dependent K+ channels with consequent K+ ion efflux and cell shrinkage.

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