Optical Nanoprobes for Ultrasensitive Immunoassay

Abstract

2006, first as a BK21 researcher and then as a research professor. In 2009, he joined the Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai as a professor. His research interests include the study of novel properties of materials such as functionalized nanoparticles for developing nanoscale biochemical analysis methods and molecular imprinting-based sample pretreatment technology.

Rapid signal enhancement method for nanoprobe-based biosensing

“…Colorimetric biosensing typically relies on the correlation between the intensity of the signal and the concentration detected to enable a quantitative analysis 28 . In this work, we show that the signal intensity for each spot concentration was observed to increase while maintaining a linear correlation to the printed analyte, and consequently, quantitative analyte estimation can be performed post-enhancement (see supporting information Table S1).…”

Machine Learning Approach to Enhance the Performance of MNP-Labeled Lateral Flow Immunoassay

“…POCT concept provides a short assay time and simple operation and is costeffective. Thus, POCT has been widely developed for early diagnosis over the past decade [15][16][17][18][19][20].…”

Interfacial Assembly of Signal Amplified Multienzymes and Biorecognized Antibody into Proteinosome for an Ultrasensitive Immunoassay

“…Biosensors as a kind of significant device combine specific recognition elements with physical or chemical transducers, which have attracted ever‐growing attention for broad fascinating applications, ranging from clinical diagnosis to environmental monitoring . The most popular biosensor type, enzyme‐linked immunosorbent assay (ELISA) relies on enzyme‐antibody conjugates to capture model analytes and then amplifies the output signal of biorecognition . Although ELISA is featured with acceptable sensitivity and specificity, its deficiencies are also conspicuous .…”

“…What's more, the sensitivity is difficult to reach picogram mL −1 level and unable to meet the detection demand, which has become a bottleneck for the monitoring of low‐abundant targets. Accordingly, obtaining new output signal amplification protocols consider as the key tasks for improving the sensitivity . To this regard, the core strategy for enhancing the sensitivity is to not only carry numerous of enzymes, but also guarantee their bioactivity.…”