Optical properties of human colon tissues in the 350 – 2500 nm spectral range

Bashkatov, Alexey N.; Genina, Elina A.; Kochubey, Vyacheslav I.; Rubtsov, Vladimir; Kolesnikova, E. A.; Tuchin, Valery V.

doi:10.1070/qe2014v044n08abeh015613

Cited by 62 publications

(37 citation statements)

References 34 publications

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“…[24][25][26][27] The absorption and scattering coefficients of other mucosal tissues, such as cervical tissue, colon tissue, and maxillary mucous membrane, have spectrum shapes similar to those of the gastric mucosa. [28][29][30] The absorption peaks of the gastric mucosa are wavelengths of around 420 and 550 nm, whereas those of other mucosal tissues are wavelengths of around 410, 550, and 575 nm unlike the absorption spectrum shape of the gastric mucosa. Therefore, the excitation light with the wavelength of 505 nm may be effective for deep intramucosal cancer in other mucosal tissues because the absorption peak of mucosal tissues almost coincides with that of PpIX except for the wavelength of 505 nm.…”

Section: Discussionmentioning

confidence: 99%

Fluorescence detection of deep intramucosal cancer excited by green light for photodynamic diagnosis using protoporphyrin IX induced by 5-aminolevulinic acid: an ex vivo study

et al. 2020

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Fluorescence detection of deep intramucosal cancer excited by green light for photodynamic diagnosis using protoporphyrin IX induced by 5-aminolevulinic acid: an ex vivo study," J. AbstractSignificance: The diagnostic depth of photodynamic diagnosis (PDD) for gastric cancer with protoporphyrin IX (PpIX) is limited, which leads to missing intramucosal cancers in screening and surgery.Aim: The reason is that the excitation light, whose wavelength is determined by the highest absorption peak of PpIX (∼405 nm), is strongly attenuated by mucosal tissues. We investigated an excitation wavelength that can extend the diagnostic depth of PpIX fluorescence at the mucosal subsurface.Approach: By calculating the depth-dependent intensity of the excitation light in porcine gastric mucosa for each wavelength, relationships among the wavelength, fluorophore depth, and fluorescence intensity were assessed and fluorescence images of PpIX pellets located at different fluorophore depths were compared experimentally by changing the excitation wavelength.Results: The numerical calculation showed that a 505-nm excitation light provided the highest fluorescence intensities at a fluorophore depth deeper than 1.1 mm. In the fluorescence observation, the fluorescence intensities at fluorophore depths of 0 and 1.0 mm at 405 nm were 5.4 × 10 3 and 1.0 × 10 3 arb: units, whereas those at 505 nm were 5.3 × 10 1 and 1.9 × 10 2 arb: units, respectively. Conclusion:The experimental results suggest that the diagnosis depth of PDD with PpIX for intramucosal cancer can be extended by 505-nm excitation light.

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Section: Discussionmentioning

confidence: 99%

Fluorescence detection of deep intramucosal cancer excited by green light for photodynamic diagnosis using protoporphyrin IX induced by 5-aminolevulinic acid: an ex vivo study

et al. 2020

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“…Such composition for the muscle indicates that a significant RI mismatch exists between the proteins (RI ~ 1.53) and the muscle fluids (RI ~ 1.35). Such mismatch is responsible for strong light scattering inside the muscle in the UV‐near infrared (NIR) range , which will originate significant power loss in a light beam or low contrast images obtained from small tissue depths .…”

Section: Methodsmentioning

confidence: 99%

Skeletal muscle dispersion (400‐1000 nm) and kinetics at optical clearing

Oliveira

Carvalho

Nogueira

et al. 2017

Journal of Biophotonics

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Skeletal muscle dispersion and optical clearing (OC) kinetics were studied experimentally to prove the existence of the refractive index (RI) matching mechanism of OC. Sample thickness and collimated transmittance spectra were measured during treatments with glucose (40%) and ethylene glycol (EG; 99%) solutions and used to obtain the time dependence of the RI of tissue fluids based on the proposed theoretical model. Calculated results demonstrated an increase of RI of tissue fluids and consequently proved the occurrence of the RI matching mechanism. The RI increase was observed for the wavelength range between 400 and 1000 nm and for the 2 probing molecules explored. We found that for 30 min treatment with 40% glucose and 99% EG, RI of sarcoplasm plus interstitial fluid was increased at 800 nm from 1.328 to 1.348 and from 1.328 to 1.369, respectively.

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“…To create synthetic test data we simulated multispectral image data which was subsequently filtered to generate RGB camera responses. The optical characteristics of blood and colonic submucosa (soft tissue) were compiled from [3] and [2] respectively. The synthetic phantom model comprised a homogeneous block of soft tissue with three superficial vessels containing either oxygenated or deoxygenated blood.…”

Section: Synthetic Evaluationmentioning

confidence: 99%

Inference of Tissue Haemoglobin Concentration from Stereo RGB

Jones

Clancy

Arridge

et al. 2016

Lecture Notes in Computer Science

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Multispectral imaging (MSI) can provide information about tissue oxygenation, perfusion and potentially function during surgery. In this paper we present a novel, near real-time technique for intrinsic measurements of total haemoglobin (THb) and blood oxygenation (SO2) in tissue using only RGB images from a stereo laparoscope. The high degree of spectral overlap between channels makes inference of haemoglobin concentration challenging, non-linear and under constrained. We decompose the problem into two constrained linear sub-problems and show that with Tikhonov regularisation the estimation significantly improves, giving robust estimation of the THb. We demonstrate by using the co-registered stereo image data from two cameras it is possible to get robust SO2 estimation as well. Our method is closed from, providing computational efficiency even with multiple cameras. The method we present requires only spectral response calibration of each camera, without modification of existing laparoscopic imaging hardware. We validate our technique on synthetic data from Monte Carlo simulation and further, in vivo, on a multispectral porcine data set.

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Optical properties of human colon tissues in the 350 – 2500 nm spectral range

Cited by 62 publications

References 34 publications

Fluorescence detection of deep intramucosal cancer excited by green light for photodynamic diagnosis using protoporphyrin IX induced by 5-aminolevulinic acid: an ex vivo study

Fluorescence detection of deep intramucosal cancer excited by green light for photodynamic diagnosis using protoporphyrin IX induced by 5-aminolevulinic acid: an ex vivo study

Skeletal muscle dispersion (400‐1000 nm) and kinetics at optical clearing

Inference of Tissue Haemoglobin Concentration from Stereo RGB

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