Optical rotatory dispersion and circular dichroism of rice dwarf virus ribonucleic acid

Samejima, Tatsuya; Hashizume, Hiroshi; Imahori, Kazutomo; Fujii, Itsuhiro; Miura, Kiyonori

doi:10.1016/0022-2836(68)90233-7

Cited by 62 publications

(17 citation statements)

References 19 publications

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“…Over the range 220-300nm the specific rotations of fractions 1 and 2 are very similar, as are the values of ellipticity. The circular dichroism of fractions 1 and 2 is similar to that found for rice dwarf virus RNA (Samejima, Hashizume, Imahori, Fujii & Miura, 1968), which is known to have a double-helical structure.…”

Section: Fractionsupporting

confidence: 66%

Double-helical character of ribonucleic acid from virus-like particles found in Penicillium chrysogenum

Cox

Kanagalingam

Sutherland

1970

Biochemical Journal

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1. RNA was isolated from virus-like particles found in Penicillium chrysogenum and resolved into two fractions by gel filtration through agarose columns. 2. Fraction 1 was excluded and had the following properties: 50.9% G+C [AMP 0.246, UMP 0.246, CMP 0.252, GMP 0.255 (mole fraction)]; mol.wt. about 1.2x10(6) daltons; s(20,w) 12.3S and ;melting' temperature about 100 degrees C (solvent 0.15m-sodium chloride-0.015m-sodium citrate pH7.2); optical rotation [alpha](max.) 6000 degrees at 278nm; circular dichroism (epsilon(L)-epsilon(R))(max.)=8.181mol(-1) cm(-1) at 260nm. 3. Properties of fraction 2 include 37.8% G+C [AMP 0.313, UMP 0.312, CMP 0.186, GMP 0.189 (mole fraction)]; mol.wt. about 140000 daltons; s(20,w) 7.3S, T(m) about 85 degrees C (solvent 0.15m-sodium chloride-0.015m-sodium citrate, pH7.2); optical rotation [alpha](max.) 6000 degrees at 278nm; circular dichroism (epsilon(L)-epsilon(R))(max.)=8.241mol(-1) cm(-1) at 260nm. 4. The properties of both fractions were consistent with a double-helical conformation.

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Section: Fractionsupporting

confidence: 66%

Double-helical character of ribonucleic acid from virus-like particles found in Penicillium chrysogenum

Cox

Kanagalingam

Sutherland

1970

Biochemical Journal

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“…The temperature corresponding to the midpoint of the absorbance rise, the melting temperature (T,), was 80 C for CPV RNA. This is quite similar to the TCn of rice dwarf virus RNA (29,34). The appearance of the absorption rise is similar to that of natural double-stranded DNA.…”

Section: Resultssupporting

confidence: 72%

“…7) showed an intense positive band at 260 nm; close to the crossover point of the Cotton effect the intensity of the band was more prominent than that of a single-stranded RNA. These features are quite similar to those of the doublestranded rice dwarf virus RNA (34), but are different from normal single-stranded RNA or double-stranded DNA (3,35). After CPV RNA was denatured by heating at 00 C for 10 min in 0.01 X SSC (and quickly cooled in ice), the magnitude of the Cotton effect and the intensity of the circular dichroism band decreased to the value obtained in a normal singlestranded RNA (Fig.…”

Section: Resultsmentioning

confidence: 70%

Double-stranded Ribonucleic Acid from Cytoplasmic Polyhedrosis Virus of the Silkworm

Miura

Fujii

Sakaki

et al. 1968

J Virol

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Ribonucleic acid (RNA) was extracted by phenol treatment from cytoplasmic polyhedrosis virus isolated from the midgut of infected silkworms. This RNA appears as threads when precipitated in alcohol. Two components having different sedimentation constants were observed. The molecular weight of the RNA preparation obtained by sedimentation coefficient (weight-averaged) and intrinsic viscosity was about 2 X 106 to 3 X 106. It was one-half to one-third the size of the calculated molecular weight for an entire RNA molecule in a virion. Electron micrographs of this RNA preparation showed two peaks in the distribution of contour length, at 0.4 and 1.3 Am, which would correspond to molecular weights of 106 and 3 X 106, respectively. The extracted RNA seemed to split into segments at a preferential breaking point. This RNA was soluble in concentrated salt solution, differing from single stranded high-molecular-weight RNA. The base composition of this RNA was complementary in the ratios of adenosine to uridine and guanosine to cytosine. It contained 43 %guanosine plus cytosine. Based on its filamentous appearance by electron microscopy, typical pattern of optical rotatory dispersion and circular dichroism, sharp transition of the optical properties on heating, great hyperchromicity on degradation, nonreactivity with formaldehyde, and resistance to ribonucleases, it is concluded that this RNA is double-stranded and has regular base pairings of guanosine-cytosine and adenosine-uridine.Cytoplasmic polyhedrosis disease in insects results in inclusion bodies, polyhedra, which contain many virus particles. When the nucleic acid extracted from the cytoplasmic polyhedra formed in the midgut epithelium of the infected silkworm was precipitated in ethyl alcohol, fibrous precipitates were obtained in addition to flocculent precipitates (14), both of which were proven to be ribonucleic acid (RNA) by color reactions for sugar components and by digestion with nucleases. The fibrous precipitate fraction, however, was different from the ordinary cellular RNA species in its elution profile on a methylated albumin column and in its complementary base composition (15).Recently, virus particles were liberated from cytoplasmic polyhedra with the use of a carbonate buffer (pH 10.8) and were purified by ultracentrifugation (16). The nucleic acid extracted by phenol treatment from this virus preparation produced only a fibrous precipitate in alcohol, and no flocculent precipitate. The properties of this fibrous nucleic acid have now been studied, and it has been concluded that this nucleic acid is a double-stranded RNA like the RNA from reovirus (11, 22), from wound tumor virus (2, 43), and from rice dwarf virus (29, 36). MATERIALS AND METHODSPurification of virus. On the 1st day of 5th instar, silkworms, Bombyx moni (L.), were injected in the posterior region with virus suspension, which was obtained by dissolving cytoplasmic polyhedra in 0.05 M Na2CO3-0.05 M NaCl (S. Kawase and S. Miyajima, J. Invert. Pathol., in press). A few days later, midgu...

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“…6 it appears that heat induces a significant red shift of the peak at the longer wavelengths. This was attributed by Hashizume & Imahori (1967), Samejima et al (1968) and Adams, Lindahl & Fresco (1967) to the separation of paired bases through the breaking of hydrogen bonds, whereas the unstacking of the bases markedly reduces the intensity of the CD bands. The red shift of the band on melting for both virus and RNA is illustrated in Fig.…”

Section: Spectra Of Virus and Its Componentsmentioning

confidence: 92%

Circular Dichroism Studies of Chicory Yellow Mottle Virus

Piazzolla¹,

Guantieri

Vovlas³

et al. 1977

Journal of General Virology

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SUMMARYA spectroscopic characterization is given of the isometric RNA-containing chicory yellow mottle virus. The circular dichroism of the virus is close to the sum of that of the native RNA and protein components. The nucleic acid structure is formed by regions of single-chain stacked-base helices similar to that of poly(A) at neutral pH, and short double-helical loops, while the conformation of the capsid is largely accounted for by regions of fl-form and random-chain structures.The heat-induced dissociation of virus nucleoprotein occurs concomitantly with destabilization of the orderly structures of the protein moiety, indicating that only minor interactions between RNA and protein occur. It is suggested that interactions between RNA and protein could involve some aromatic residues of the capsid and the heterocyclic bases of the nucleic acid.

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Optical rotatory dispersion and circular dichroism of rice dwarf virus ribonucleic acid

Cited by 62 publications

References 19 publications

Double-helical character of ribonucleic acid from virus-like particles found in Penicillium chrysogenum

Double-helical character of ribonucleic acid from virus-like particles found in Penicillium chrysogenum

Double-stranded Ribonucleic Acid from Cytoplasmic Polyhedrosis Virus of the Silkworm

Circular Dichroism Studies of Chicory Yellow Mottle Virus

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