Optical sensor for hydrogen peroxide

Posch, Hermann E.; Wolfbeis, Otto S.

doi:10.1007/bf01197282

Cited by 43 publications

(23 citation statements)

References 21 publications

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“…There are various mechanisms converting H 2 O 2 in oxygen, which then results in an increase of measured O 2 . This can be achieved e.g., by covering a planar O 2 sensitive layer based on luminescence quenching with a second layer containing an inorganic catalyst (such as MnO 2 [143], or finely dispersed Ag-powder), by co-absorbing catalase in silica gel/the same phase as the indicator layer, or alternatively, by adsorption of enzyme and dye on silica gel particles, which are then immobilized in a polymer layer [144] (Figure 7). This method has one clear advantage against all the other methods described above; the sensing system it relies on is truly reversible.…”

Section: Fully Reversible Optical Chemical Sensorsmentioning

confidence: 99%

Possibilities and Challenges for Quantitative Optical Sensing of Hydrogen Peroxide

2017

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Abstract:Hydrogen peroxide (H 2 O 2 ) plays a key role in many biological processes spanning from coral bleaching, over cell signaling to aging. However, exact quantitative assessments of concentrations and dynamics of H 2 O 2 remain challenging due to methodological limitationsespecially at very low (sub µM) concentrations. Most published optical detection schemes for H 2 O 2 suffer from irreversibility, cross sensitivity to other analytes such as other reactive oxygen species (ROS) or pH, instability, temperature dependency or limitation to a specific medium. We review optical detection schemes for H 2 O 2 , compare their specific advantages and disadvantages, and discuss current challenges and new approaches for quantitative optical H 2 O 2 detection, with a special focus on luminescence-based measurements. We also review published concentration ranges for H 2 O 2 in natural habitats, and physiological concentrations in different biological samples to provide guidelines for future experiments and sensor development in biomedical and environmental science.

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Section: Fully Reversible Optical Chemical Sensorsmentioning

confidence: 99%

Possibilities and Challenges for Quantitative Optical Sensing of Hydrogen Peroxide

2017

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“…Such operations can be automated and are designed to identify any effect of KCN addition upon the region of the oxygen sensing layer in the vicinity of the cell being analyzed. In both cases, the luminescence intensity increased following the addition of KCN, which is consistent with a decrease in the local oxygen tension (i.e., cellular oxygen consumption) and/or an increase in the local concentration of reactive oxygen species (e.g., hydrogen peroxide, which is also known to quench the luminescence of ruthenium-based dyes (71)). Such behavior is also consistent with electrochemical observations of oxidative stress events where the release of peroxides from a single cell coincided with cellular oxygen consumption on a millisecond timescale (33).…”

Section: Imaging Live Cellsmentioning

confidence: 76%

Combined Imaging and Chemical Sensing with a Disposable Microscope Objective Chemical Sensor

Hartzell¹,

Danowski²,

Pantano³

2007

Applied Spectroscopy Reviews

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This manuscript provides a concise review of the combined imaging and chemical sensing (CICS) technique performed with imaging fiber chemical sensors (IFCSs) and represents the first demonstration of CICS using a disposable microscope objective chemical sensor (dMOCS). The dMOCS assembly comprises two pieces that clamp around an imaging system's objective and a third adjoining piece that positions a sensing layer onto a sample's surface and within the objective's field of view. The prototype layer was oxygen-sensitive and was fabricated using a luminescent ruthenium metal complex and a gas permeable polymeric membrane. O 2 -sensitive dMOCSs were characterized with respect to their imaging capabilities, sensitivity, and temporal response, and the feasibility of performing a high-content screening assay was demonstrated with preliminary measurements of oxygen dynamics from living cells. While IFCSs are a requisite for analyzing remote samples that cannot be brought to a microscope stage, for those samples that can, CICS with a dMOCS possesses advantages with respect to spatial resolution, ease of fabrication, cost, and viewing area.

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“…Optical sensors have been widely used as gas sensors in many applications due to their response which could be measured precisely [16][17][18]. These sensors are based on a light source that excites the volatile molecules, and the signal can be measured in the resulting absorbance, reflectance, fluorescence, or chemiluminescence.…”

Section: Optical Sensorsmentioning

confidence: 99%