Optical Sensor for Real‐Time pH Monitoring in Human Tissue

Wencel, Dorota; Kaworek, Alicja; Abel, Tobias; Efremov, Vitaly; Bradford, Aidan; Carthy, Denise; Coady, Garret; McMorrow, Roger; McDonagh, Colette

doi:10.1002/smll.201803627

Cited by 55 publications

(45 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fig. 8 and 9 show the ∆P and s P values obtained by analyzing the N s = 6 sensors according to (13) and (14). According to the results obtained by analyzing the absorbance spectra and previously shown in Fig.…”

Section: B Substrate Fluorescence and Calibration Curvementioning

confidence: 63%

See 1 more Smart Citation

Performances and Robustness of a Fluorescent Sensor for Nearly Neutral pH Measurements in Healthcare

Cattini

Truzzi

Accorsi

et al. 2020

IEEE Trans. Instrum. Meas.

View full text Add to dashboard Cite

The capability to measure pH is fundamental in many fields ranging from healthcare and agri-food, to chemistry and industrial applications. In medicine, the possibility of in-line and real-time monitoring critical care analytes such as pH is recognized to provide relevant information for the diagnosis and treatment of a variety of disorders and would be of considerable support for the management of extracorporeal (blood) circulation (ECC). In this paper, we theoretically and experimentally investigate the performances and the robustness of a fluorescent sensor we recently proposed for the in-line and real-time monitoring of blood pH in extracorporeal circulation (ECC). Thanks to the low-cost and biocompatibility, the proposed sensor can be used for the in-line and real-time monitoring of the pH of fluids especially in applications such as healthcare where safe and low-cost disposable are required for all parts in contact with the patient. The reported results demonstrate that the proposed sensor allows achieving significant robustness to unevennesses in the production process of the sensing element.

show abstract

Section: B Substrate Fluorescence and Calibration Curvementioning

confidence: 63%

“…∆P values obtained from the Ns = 6 sensors (PBS solution 1X, at 27 ○ C). The reported values have been obtained according to (13) and analyzing the two peaks in Fig. 7.…”

Section: B Substrate Fluorescence and Calibration Curvementioning

confidence: 99%

Performances and Robustness of a Fluorescent Sensor for Nearly Neutral pH Measurements in Healthcare

Cattini

Truzzi

Accorsi

et al. 2020

IEEE Trans. Instrum. Meas.

View full text Add to dashboard Cite

show abstract

“…In recent years, optical sensors have become a convenient alternative in several application areas of research, including biological systems and organisms. Different principles and working mechanisms are used depending on the analyte to be measured [26] and for optical pH and CO 2 measurements, a combination of different uorescent dyes which detect intensity changes in the time domain are used. Although optical based probes were used to assess in vivo oxygen levels within the female reproductive tract of humans [27] and pigs [28], to our knowledge, this technology has never been used in reproductive organs for pH and CO 2 measurement.…”

Section: Speciesmentioning

confidence: 99%

In vivo measurement of pH, CO2 and HCO3- levels in the uterus of non-anesthetized sows through the estrous cycle and after insemination

López-Albors¹,

Llamas-López²,

Ortuño³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background The pH-CO2-HCO3− system is a ubiquitous biological regulator with important functional implications for reproduction. Knowledge of the physiological values of its components is relevant for reproductive biology and the optimization of Assistant Reproductive Techniques (ARTs). In vivo pH of the oviduct and uterus has been estimated by direct in situ measurements in a few species. However, regarding the levels of CO2 and HCO3−, information is very scarce and, when available, it comes from fluid samples instead of in vivo estimations. This study describes a non-invasive method to measure pH and % of CO2 in the uterus of sows with cutting-edge technology and no medication. Sows were at three different reproductive conditions, estrous with no insemination E(-)AI and after insemination E(+)AI, and diestrous (non-estrous, NE). From pH and CO2 data, HCO3− concentration was estimated. Results The designed methodology allowed for in situ time-lapse recording of pH and % of CO2 within the uterus of non-anesthetized sows. Variable oscillatory patterns of pH, CO2 and HCO3− were found independently of the estrous condition. Insemination did not changed the levels of uterine pH, % of CO2 and HCO3− concentration, -E(-)AI = E(+)AI-, but all the values were affected by the estrous cycle in a way that decreased significantly at diestrous condition - E(-)AI and E(+)AI > NE-. Conclusions A non-invasive approach to the porcine uterus with novel optical probes allowed the obtaining of in situ physiological values of pH, CO2, and HCO3− at different reproductive conditions. While the short-time presence of sperm in the uterus did not change the physiological milieu, the whole pH-CO2-HCO3− system was affected by the estrous cycle. This study contributes to a better understanding of the in vivo regulation of the pH/CO2/HCO3− system in the uterus and may help to optimize the protocols of sperm treatment for in vitro fertilization.

show abstract

“…In order to illustrate the performances of the sensor, the characteristics of the LC-based sensors were compared to other reported methods. As mentioned before, the pH monitoring sensors mainly include the iodometric method, spectrophotometry, the mercury amount method, and electrochemical methods [25][26][27][28][29][30][31][32][33][34] . Compared to these methods, the sensors we develop are simple, economical, fast, efficient, and can be used for in real-time monitoring for a long time, while and no other aids are needed.…”

Section: Introductionmentioning

confidence: 99%

Liquid-crystal-droplet-based Monitoring System for Water-soluble Inorganic Acidic Gases from the Atmosphere

Jang

2020

BioChip J

View full text Add to dashboard Cite

A liquid crystal (LC)-based pH sensor for real-time monitoring of variations in localized pH values near the LC droplets was developed, and its applicability for the detection of acidic gases was explored. It was found that 4-cyano-4′-pentylbiphenyl (5CB), when doped with hexanoic acid (HA), shows a bright-fan-shaped (planar orientation of LCs) to dark-cross (homeotropic orientation of LCs) optical response to a minimal change of the pH value (from 6.5 to 6.6). The fast and intensive pH-driven optical response can be explained mainly with the orientational transitions of 5CB, induced by the protonation and deprotonation of HA at the interface of aqueous/LC droplet. Because of its high pH sensitivity, the LC-based sensor was further exploited for monitoring the local pH changes, which were originated from concentration changes of acidic gases. The results suggested that at an industrial scale/production environment, the sensor shows excellent performance for long-term monitoring and sensing of acidic gases. This type of LC-based sensor may find to fulfill its applicability potential in the trace measurement of acidic gases.

show abstract

Optical Sensor for Real‐Time pH Monitoring in Human Tissue

Cited by 55 publications

References 36 publications

Performances and Robustness of a Fluorescent Sensor for Nearly Neutral pH Measurements in Healthcare

Performances and Robustness of a Fluorescent Sensor for Nearly Neutral pH Measurements in Healthcare

In vivo measurement of pH, CO2 and HCO3- levels in the uterus of non-anesthetized sows through the estrous cycle and after insemination

Liquid-crystal-droplet-based Monitoring System for Water-soluble Inorganic Acidic Gases from the Atmosphere

Contact Info

Product

Resources

About