Optical sensor systems for bioprocess monitoring

Marose, Stefan

doi:10.1016/s0167-7799(98)01247-5

Cited by 100 publications

(70 citation statements)

References 30 publications

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“…[63][64][65][66][67] Both methods have been successfully applied for determination of biomass concentration online; [68][69][70][71] however, with multiwavelength fluorescence it is also possible to monitor other compounds such as products [72][73][74][75] under the condition that they are fluorescent.…”

Section: Discussionmentioning

confidence: 99%

Application of near‐infrared spectroscopy for monitoring and control of cell culture and fermentation

Cervera

Petersen

Lantz

et al. 2009

Biotechnology Progress

145

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Near-infrared (NIR) spectroscopy can potentially provide on-line information on substrate, biomass, product, and metabolite concentrations in fermentation processes, which could be useful for improved monitoring or control. However, several factors can negatively influence the quality of chemometric models built for interpretation of the spectra, thus impairing the analyte concentration predictions. The aim of this review was to provide an overview of necessary conditions and challenges that one has to face when developing a NIR application for monitoring of cell culture or fermentation processes. Important practical aspects are introduced, such as sampling, modeling of biomass concentration, influence of microorganism morphology on the spectra, effects of the hydrodynamic conditions in the fermenter, temperature influence, instrument settings, and signal optimization. Several examples from the literature are provided, which will hopefully guide the reader interested in the topic. Furthermore, the general procedure used for the development of calibration models is presented, and the influence of microorganism metabolism-potential source of correlation between analytes-is commented. Other important issues such as wavelength selection and evaluation of robustness are shortly introduced. Finally, some examples of potential applications of NIR monitoring are provided, including the implementation of control strategies, the combination with other monitoring tools (the so-called sensor fusion), and the description of process trajectories. On the basis of the review, we conclude that acceptance of NIR spectroscopy as a standard monitoring tool by the fermentation industry will necessitate considerably more on-line studies using industrially relevant-and highly challenging-fermentation conditions (high aeration intensity, high biomass concentration and viscosity, and filamentous production strain).

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Section: Discussionmentioning

confidence: 99%

Application of near‐infrared spectroscopy for monitoring and control of cell culture and fermentation

Cervera

Petersen

Lantz

et al. 2009

Biotechnology Progress

145

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“…Fiber-optic sensors, also known as optodes, are based on the change in the optical properties of a particular indicator, such as a dye. Biological compounds can be coupled to the optode for the detection of targets, like glucose and hydrogen peroxide [16]. Near-infrared spectroscopy can quantify the concentration of certain organic species by the analysis of the adsorption bands in the spectrum [17].…”

Section: Transduction Mechanismmentioning

confidence: 99%

A Self-Contained System With CNTs-Based Biosensors for Cell Culture Monitoring

Boero

Olivo

Carrara

et al. 2012

IEEE J. Emerg. Sel. Topics Circuits Syst.

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Abstract-Biosensors have been applied to disparate fields, especially for endogenous compounds such as glucose and lactate. The main areas of application are certainly related to medical and diagnostic purposes. However, metabolic monitoring can be also of interest in cell analysis. Cells can be cultivated for several purposes, such as understanding and modeling some biological mechanisms, the development of new drugs and therapies, or in the field of regenerative medicine. All the aforementioned applications require a thorough knowledge of the biological system under study. In this paper, we propose the development of a self-contained system based on electrochemical biosensors for cell culture monitoring. The detection is based on oxidases immobilized onto carbon nanotubes. We also develop an architecture to record the signal generated by the biosensor and transmit it to a remote station by means of a Bluetooth module. We calibrate the system for glucose and lactate detection in phosphate buffer solution. We achieve a sensitivity of 55.5 and a detection limit of 2 for glucose, as well as a sensitivity of 25.0 and a detection limit of 11 for lactate. We finally validate the two biosensors for metabolic monitoring in culture medium and we detect lactate production in neuroblastoma cells after 72 h of cultivation. The integrated system proposed in the present work opens new opportunities towards the development of novel tools for cell analysis.

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“…In-situ-Fluoreszenzsensoren haben sich sowohl in der Forschung und Entwicklung als auch in der industriellen Anwendung bewährt [75,76]. Einige kommerziell erhältliche Fluorosensoren (z.…”

Section: Fluorimetrieunclassified